Hydrophobic Amino Acid Residues in the Acceptor Binding Site Are Main Determinants for Reaction Mechanism and Specificity of Cyclodextrin-glycosyltransferase

Journal of Biological Chemistry

Volumn 276, Issue 48, 2001, Pages 44557-44562

  Van der Veen, Bart A ^a,b   Leemhuis, Hans ^a   Kralj, Slavko ^a,b   Uitdehaag, Joost C M ^a   Dijkstra, Bauke W ^a   Dijkhuizen, Lubbert ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b TNORUG   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; ENZYMES; HYDROLYSIS; HYDROPHOBICITY; MUTAGENESIS; STABILIZATION;

TRANSGLYCOSYLATION;

AMINO ACIDS;

AMINO ACID; AMYLASE; CYCLOMALTODEXTRIN GLUCANOTRANSFERASE; WATER; ASPARTIC ACID; BETA CYCLODEXTRIN; CYCLODEXTRIN; GLUCOSYLTRANSFERASE; GLUTAMIC ACID; HISTIDINE; LYSINE; PHENYLALANINE;

ARTICLE; BACILLUS CIRCULANS; BACTERIAL STRAIN; BINDING SITE; CATALYSIS; CYCLIZATION; ENZYME SPECIFICITY; GLYCOSYLATION; HYDROLYSIS; HYDROPHOBICITY; MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; REACTION ANALYSIS; CHEMICAL STRUCTURE; CHEMISTRY; DOSE RESPONSE; ENZYMOLOGY; ESCHERICHIA COLI; KINETICS; METABOLISM; PLASMID; PROTEIN BINDING; SITE DIRECTED MUTAGENESIS;

BACILLUS CIRCULANS; BACTERIA (MICROORGANISMS);

AMINO ACIDS; ASPARTIC ACID; BINDING SITES; CYCLODEXTRINS; DOSE-RESPONSE RELATIONSHIP, DRUG; ESCHERICHIA COLI; GLUCOSYLTRANSFERASES; GLUTAMIC ACID; HISTIDINE; HYDROLYSIS; KINETICS; LYSINE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; PHENYLALANINE; PLASMIDS; PROTEIN BINDING; PROTEIN CONFORMATION; SUBSTRATE SPECIFICITY; SUPPORT, NON-U.S. GOV'T; WATER;

EID: 0035976993     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M107533200     Document Type: Article

References (26)

1. Henrissat, B. (1991) Biochem. J. 280, 309-316
2. Uitdehaag, J. C. M., Mosi, R., Kalk, K. H., van der Veen, B. A., Dijkhuizen, L., Withers, S. G., and Dijkstra, B. W. (1999) Nat. Struct. Biol. 6, 432-436
3. van der Veen, B. A., van Alebeek, G. J. W. M., Uitdehaag, J. C. M., Dijkstra, B. W., and Dijkhuizen, L. (2000) Eur. J. Biochem. 267, 658-665
4. Strokopytov, B., Knegtel, R. M. A., Penninga, D., Rozeboom, H. J., Kalk, K. H., Dijkhuizen, L., and Dijkstra, B. W. (1996) Biochemistry 35, 4241-4249
5. Allegre, M., and Deratani, A. (1994) Agro Fd. Ind. 5, 9-17
6. Brzozowski, A. M., and Davies, G. J. (1997) Biochemistry 36, 10837-10845
7. Nakamura, A., Haga, K., and Yamane, K. (1994) Biochemistry 33, 9929-9936
8. Wind, R. D., Uitdehaag, J. C. M., Buitelaar, R. M., Dijkstra, B. W., and Dijkhuizen, L. (1998) J. Biol. Chem. 273, 5771-5779
9. Uitdehaag, J. C. M., Kalk, K. H., van der Veen, B. A., Dijkhuizen, L., and Dijkstra, B. W. (1999) J. Biol. Chem. 274, 34868-34876
10. Meissner, P. S., Sisk, W. P., and Berman, M. L. (1987) Proc. Natl. Acad. Sci. U. S. A. 84, 4171-4175
11. Hanahan, D. (1983) J. Mol. Biol. 166, 557
12. Smith, H., de Jong, A., Bron, S., and Venema, G. (1988) Gene 70, 351-361
13. Penninga, D., van der Veen, B. A., Knegtel, R. M. A., van Hijum, S. A. F. T., Rozeboom, H. J., Kalk, K. H., Dijkstra, B. W., and Dijkhuizen, L. (1996) J. Biol. Chem. 271, 32777-32784
14. van de Vossen, J. M. B. M., Kodde, J., Haandrikman, A. J., Venema, G., and Kok, J. (1992) Appl. Environ. Microbiol. 58, 3142-3149
15. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning, a Laboratory Manual, Cold Spring Harbor Laboratory Press, New York
16. Bron, S. (1990) in Modern Microbiological Methods for Bacillus (Harwood, C. R. and Cutting, S. M., eds) pp. 146-147, John Wiley & Sons, Inc., New York
17. Voss, H., Schwager, C., Wirkner, U., Zimmermann, J., Erfle, H., Hewitt, N., Rupp, T., Stegemann, J., and Ansorge, W. (1992) Methods Mol. Cell. Biol. 3, 30-34
18. Zimmermann, J., Voss, H., Schwager, C., Stegemann, J., Erfle, H., Stucky, K., Kristensen, T., and Ansorge, W. (1990) Nucleic Acids Res. 18, 1067
19. Sundberg, L., and Porath, J. (1974) J. Chromatogr. 90, 87-98
20. Vikmon, M. (1982) in First International Symposium on Cyclodextrins (Szejtli, J., ed) pp. 69-74, D. Reidel Publishing, Dordrecht, The Netherlands
21. Penninga, D., Strokopytov, B., Rozeboom, H. J., Lawson, C. L., Dijkstra, B. W., Bergsma, J., and Dijkhuizen, L. (1995) Biochemistry 34, 3368-3376
22. Bernfeld, P. (1955) Methods Enzymol. 1, 149-158
23. Cornish-Bowden, A. (1995) Fundamentals of Enzyme Kinetics (Cornish-Bowden, A., ed) Portland Press, London
24. Nakamura, A., Haga, K., and Yamane, K. (1993) Biochemistry 32, 6624-6631
25. Uitdehaag, J. C. M., van Alebeek, G. J. W. M., van der Veen, B. A., Dijkhuizen, L., and Dijkstra, B. W. (2000) Biochemistry 39, 7772-7780
26. Uitdehaag, J. C. M., van der Veen, B. A., Dijkhuizen, L., Elber, R., and Dijkstra, B. W. (2001) Proteins 43, 327-335