Improving maltodextrin specificity for enzymatic synthesis of 2-O-d-glucopyranosyl-l-ascorbic acid by site-saturation engineering of subsite-3 in cyclodextrin glycosyltransferase from Paenibacillus macerans

Journal of Biotechnology

Volumn 166, Issue 4, 2013, Pages 198-205

  Liu, Long ^a   Xu, Qiaoyan ^a   Han, Ruizhi ^a   Shin, Hyun dong ^b   Chen, Rachel R ^b   Li, Jianghua ^a   Du, Guocheng ^a   Chen, Jian ^a  

^a JIANGNAN UNIVERSITY   (China)

^b Georgia Institute of Technology   (United States)

Author keywords

2 O Glucopyranosyl l ascorbic acid (AA 2G); Cyclodextrin glycosyltransferase (CGTase); L Ascorbic acid (l AA); Maltodextrin; Site saturation mutagenesis

Indexed keywords

2-O-GLUCOPYRANOSYL-L-ASCORBIC ACID (AA-2G); CYCLODEXTRIN GLYCOSYLTRANSFERASE; ENZYMATIC SYNTHESIS; HYDROGEN BONDING INTERACTIONS; L-ASCORBIC ACID; MALTODEXTRINS; PAENIBACILLUS MACERANS; STRUCTURE MODELING;

AMINO ACIDS; CYCLODEXTRINS; HYDROGEN BONDS; MUTAGENESIS; REACTION KINETICS;

ASCORBIC ACID;

2 O DEXTRO GLUCOPYRANOSYL ASCORBIC ACID; ASPARAGINE; ASPARTIC ACID; CYCLOMALTODEXTRIN GLUCANOTRANSFERASE; MALTODEXTRIN; TYROSINE; UNCLASSIFIED DRUG;

ARTICLE; CHEMICAL REACTION KINETICS; ENZYME SYNTHESIS; HYDROGEN BOND; MUTAGENESIS; MUTANT; MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PAENIBACILLUS; PAENIBACILLUS MACERANS; PH; PRIORITY JOURNAL; THERMOSTABILITY; WILD TYPE;

2-O-GLUCOPYRANOSYL-L-ASCORBIC ACID (AA-2G); CYCLODEXTRIN GLYCOSYLTRANSFERASE (CGTASE); L-ASCORBIC ACID (L-AA); MALTODEXTRIN; SITE-SATURATION MUTAGENESIS;

AMINO ACID SUBSTITUTION; ASCORBIC ACID; BASE SEQUENCE; ENZYME STABILITY; GLUCOSYLTRANSFERASES; HYDROGEN-ION CONCENTRATION; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; PAENIBACILLUS; POLYSACCHARIDES; PROTEIN ENGINEERING; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 84880368694     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2013.05.005     Document Type: Article

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