Comparison of three-colour flow cytometry and slide-based microscopy for the scoring of micronucleated reticulocytes in rat bone-marrow and peripheral blood

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 758, Issue 1-2, 2013, Pages 12-17

  Zhou, Changhui ^a   Wang, Qingli ^b   Wang, Zheng ^a   Chang, Yan ^a  

^a National Shanghai Center for New Drug Safety Evaluation and Research   (China)

^b CENTER FOR DRUG EVALUATION   (China)

Author keywords

Aneugen; Clastogen; Cyclophosphamide; Genotoxicity; Micronucleus; Polychromatic erythrocytes

Indexed keywords

ACRIDINE ORANGE; CELLULOSE; COLCHICINE; CYCLOPHOSPHAMIDE; FLUOROURACIL; MESYLIC ACID METHYL ESTER; METHANOL; MITOMYCIN; TIOGUANINE; URETHAN; VINCRISTINE SULFATE;

ANIMAL EXPERIMENT; ARTICLE; BLOOD ANALYSIS EQUIPMENT; BLOOD CELL; BLOOD SAMPLING; BONE MARROW CELL; CELL SUSPENSION; CONTROLLED STUDY; ERYTHROCYTE; FLOW CYTOMETRY; GENOTOXICITY; INTERMETHOD COMPARISON; MALE; MICRONUCLEUS; MICRONUCLEUS TEST; MICROSCOPY; NONHUMAN; PERFORMANCE; PRIORITY JOURNAL; RAT; RETICULOCYTE; SCORING SYSTEM; SLIDE BASED MICROSCOPY; STAINING; THREE COLOR FLOW CYTOMETRY; VALIDATION STUDY;

RATTUS;

EID: 84887996575     PISSN: 13835718     EISSN: 18793592     Source Type: Journal    
DOI: 10.1016/j.mrgentox.2013.07.010     Document Type: Article

References (34)

1. Hayashi M., MacGregor J.T., Gatehouse D.G., Adler I.-D., Blakey D.H., Dertinger S.D., Krishna G., Morita T., Russo A., Sutou S. In vivo rodent erythrocyte micronucleus assay: aspects of protocol design including repeated treatments, integration with toxicity testing, and automated scoring - a report from the International Workshop on Genotoxicity Test Procedures (IWGTP). Environ. Mol. Mutagen. 2000, 35:234-252.
2. Heddle J.A. A rapid in vivo test for chromosome damage. Mutat. Res. 1973, 18:187-190.
3. International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use, Topic S2B Genotoxicity: a standard for genotoxicity testing of pharmaceuticals. ICH, Step 4 Guideline, July 16 1997.
4. International Conference on Harmonization Guidance on genotoxicity testing and data interpretation for pharmaceuticals intended for human use S2 (R1) 2011, http://www.ich.org/fileadmin/Public_Web_Site/ICH_Products/Guidelines/Safety/S2R1/Step4/S2R1_Step4.pdf (dated 30.11.11).
5. OECD Guideline for the testing of chemicals. Mammalian Erythrocyte Micronucleus Test, Guideline 474, July 1997, http://www.oecd.org/dataoecd/18/34/1948442.pdf.
6. Krishna G., Urda G., Theiss J. Principles and practices of integrating genotoxicity evaluation into routine toxicology studies: a pharmaceutical industry perspective. Environ. Mol. Mutagen. 1998, 32:115-120.
7. Hamada S., Sutou S., Morita T., Wakata A., Asanami S., Hosoya S., Ozawa S., Kondo K., Nakajima M., Shimada H., Osawa K., Kondo Y., Asano N., Sato S.-I., Tamura H., Yajima N., Marshall R., Moore C., Blakey D.H., Schechtman L.M., Weaver J.L., Torous D.K., Proudlock R., Ito S., Namiki C., Hayashi M. Evaluation of the rodent micronucleus assay by a 28-day treatment protocol: summary of the 13th Collaborative Study by the Collaborative Study Group for the Micronucleus Test (CSGMT)/Environmental Mutagen Society of Japan (JEMS)-Mammalian Mutagenicity Study Group (MMS). Environ. Mol. Mutagen. 2001, 37:93-110.
8. Schlegel R., MacGregor J.T. The persistence of micronuclei erythrocytes in the peripheral circulation of normal and splenectomized Fisher 344 rats: implications for cytogenetic screening. Mutat. Res. 1984, 127:169-174.
9. Hayashi M., Kodama Y., Awogi T., Suzuki T., Asita A.O., Sofuni T. The micronucleus assay using peripheral blood reticulocytes from mitomycin C- and cyclophosphamide-treated rats. Mutat. Res. 1992, 278:209-213.
10. Hayashi M., MacGregor J.T., Gatehouse D.G., Blakey D.H., Dertinger S.D., Abramsson-Zetterberg L., Krishna G., Morita T., Russo A., Asano N., Suzuki H., Ohyama W., Gibson D. In vivo erythrocyte micronucleus assay: III. Validation and regulatory acceptance of automated scoring and the use of rat peripheral blood reticulocytes, with discussion of non-hematopoietic target cells and a single dose-level limit test. Mutat. Res. 2007, 627:10-30.
11. Wakata A., Miyamae Y., Sato S., Suzuki T., Morita T., Asano N., Awogi T., Kondo K., Hayashi M. Evaluation of the rat micronucleus test with bone marrow and peripheral blood: summary of the 9th collaborative study by CSGMT/JEMS.MMS. Environ. Mol. Mutagen. 1998, 32:84-100.
12. Vander J.B., Ellis S.R., Harris C.A. Reticulocyte counts by means of fluorescence microscopy. J. Lab. Clin. Med. 1963, 62:132-140.
13. Dertinger S.D., Camphausen K., MacGregor J.T., Bishop M.E., Torous D.K., Avlasevich S., et al. Three-color labeling method for flow cytometric measurement of cytogenetic damage in rodent and human blood. Environ. Mol. Mutagen. 2004, 44:427-435.
14. Dertinger S.D., Torous D.K., Hall N.E., Tometsko C.R., Gasiewicz T.A. Malaria-infected erythrocytes serve as biological standards to ensure reliable and consistent scoring of micronucleated erythrocytes by flow cytometry. Mutat. Res. 2000, 464:195-200.
15. Hayashi M., Sofuni T., Ishidate M. An application of acridine orange fluorescent staining to the micronucleus test. Mutat. Res. 1983, 120:241-247.
16. Krishna G. Comparative micronucleus quantitation in pre- and post-column fractionated mouse bone marrow by manual and flow analysis. Mutat. Res. 1993, 302:119-127.
17. LeBaron M.J., Schisler M.R., Torous D.K., Dertinger S.D., Gollapudi B.B. Influence of counting methodology on erythrocyte ratios in the mouse micronucleus test. Environ. Mol. Mutagen. 2013, 54:222-228.
18. Hayashi M., MacGregor J.T., Gatehouse D.G., Adler I., Blakey D.H., Dertinger S.D., Krishna G., Morita T., Russo A., Sutou S. In vivo rodent erythrocyte micronucleus assay: Part II. Some aspects of protocol design including repeated treatments, integration with toxicity testing, and automated scoring. Environ. Mol. Mutagen. 2000, 35:234-252.
19. Dertinger S., Torous D., Hall N., Tometsko C. Measurement of cytogenetic damage in rodent blood with a single-laser flow cytometer. Curr. Protoc. Cytom. 2003, 21. Unit 7.21.
20. De Boeck M., van der Leede B., Van Goethem F., De Smedt A., Steemans M., Lampo A., Vanparys P. Flow cytometric analysis of micronucleated reticulocytes: time- and dose-dependent response of known mutagens in mice, using multiple blood sampling. Environ. Mol. Mutagen. 2005, 46:30-42.
21. Witt K.L., Livanos E., Kissling G.E., Torous D.K., Caspary W., Tice R.R., Recio L. Comparison of flow cytometry- and microscopy-based methods for measuring micronucleated reticulocyte frequencies in rodents treated with nongenotoxic and genotoxic chemicals. Mutat. Res. 2008, 649:101-113.
22. Torous D.K., Hall N.E., Murante F.G., Gleason S.E., Tometsko C.R., Dertinger S.D. Comparative scoring of micronucleated reticulocytes in rat peripheral blood by flow cytometry and microscopy. Toxicol. Sci. 2003, 74:309-314.
23. Dertinger S.D., Bishop M.E., McNamee J.P., Hayashi M., Suzuki T., Asano N., Nakajima M., Saito J., Moore M., Torous D.K., MacGregor J.T. Flow cytometric analysis of micronuclei in peripheral blood reticulocytes: I. Intra- and interlaboratory comparison with microscopic scoring. Toxicol. Sci. 2006, 94:83-91.
24. MacGregor J.T., Bishop M.E., McNamee J., Hayashi M., Asano N., Wakata A., Nakajima M., Aidoo A., Moore M.M., Dertinger S.D. Flow cytometric analysis of micronuclei in peripheral blood reticulocytes: II. An efficient method of monitoring chromosomal damage in the rat. Toxicol. Sci. 2006, 94:92-107.
25. Dertinger S.D., Miller R.K., Brewer K., Smudzin T., Torous D.K., Roberts D.J., Avlasevich S.L., Bryce S.M., Sugunan S., Chen Y. Automated human blood micronucleated reticulocyte measurements for rapid assessment of chromosomal damage. Mutat. Res. 2007, 626:111-119.
26. Harper S.B., Dertinger S.D., Bishop M.E., Lynch A.M., Lorenzo M., Saylor M., MacGregor J.T. Flow cytometric analysis of micronuclei in peripheral blood reticulocytes: III. An efficient method of monitoring chromosomal damage in the beagle dog. Toxicol. Sci. 2007, 100:406-414.
27. Hotchkiss C.E., Bishop M.E., Dertinger S.D., Slikker W., Moore M.M., MacGregor J.T. Flow cytometric analysis of micronuclei in peripheral blood reticulocytes: IV. An index of chromosomal damage in the rhesus monkey (Macaca mulatta). Toxicol. Sci. 2008, 102:352-358.
28. Dertinger S.D., Torous D.K., Hayashi M., MacGregor J.T. Flow cytometric scoring of micronucleated erythrocytes: an efficient platform for assessing in vivo cytogenetic damage. Mutagenesis 2011, 26:139-145.
29. Torous D.K., Hall N.E., Dertinger S.D., Diehl M.S., IlliLove A.H., Cederbrant K., Sandelin K., Bolcsfoldi G., Ferguson L.R., Pearson A., Majeska J.B., Tarca J.P., Hewish D.R., Doughty L., Fenech M., Weaver J.L., Broud D.D., Gatehouse D.G., Hynes G.M., Kwanyuen P., McLean J., McNamee J.P., Parenteau M., Van Hoof V., Vanparys P., Lenarczyk M., Siennicka J., Litwinska B., Slowikowska M.G., Harbach P.R., Johnson C.W., Zhao S., Aaron C.S., Lynch A.M., Marshall I.C., Rodgers B., Tometsko C.R. Flow cytometric enumeration of micronucleated reticulocytes: high transferability among 14 laboratories. Environ. Mol. Mutagen. 2001, 38:59-68.
30. ®) for the scoring of micronucleated reticulocytes in mouse peripheral blood. Environ. Mol. Mutagen. 2005, 45:44-55.
31. Hynes G.M., Torous D.K., Tometsko C.R. The single laser flow cytometric micronucleus test: a time course study using colchicine and urethane in rat and mouse peripheral blood and acetaldehyde in rat peripheral blood. Mutagenesis 2002, 17:15-23.
32. ® method for the flow cytometric analysis of micronuclei in rat bone marrow erythrocytes. Mutat. Res. 2010, 703:122-129.
33. Cammerer Z., Elhajouji A., Kirsch-Volders M., Suter W. Comparison of the peripheral blood micronucleus test using flow cytometry in rat and mouse exposed to aneugens after single-dose applications. Mutagenesis 2007, 22:129-134.
34. Kirsch-Volders M., Aardema M., Elhajouji A. Concepts of thresholds in mutagenesis and carcinogenesis. Mutat. Res. 2000, 464:3-11.