Three-color labeling method for flow cytometric measurement of cytogenetic damage in rodent and human blood

Environmental and Molecular Mutagenesis

Volumn 44, Issue 5, 2004, Pages 427-435

  Dertinger, Stephen D ^a   Camphausen, Kevin ^b   MacGregor, James T ^c   Bishop, Michelle E ^d   Torous, Dorothea K ^a   Avlasevich, Svetlana ^a   Cairns, Siân ^a   Tometsko, Carol R ^a   Menard, Cynthia ^b   Muanza, Thierry ^b   Chen, Yuhchyau ^e   Miller, Richard K ^e   Cederbrant, Karin ^f   Sandelin, Kerstin ^f   Pontén, Ingrid ^f   Bolcsfoldi, Georqe ^f  

^a LITRON LABORATORIES   (United States)

^b NATIONAL CANCER INSTITUTE   (United States)

^c CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

^d NATIONAL CENTER FOR TOXICOLOGICAL RESEARCH   (United States)

^e UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

^f ASTRAZENECA R AND D   (Sweden)

Author keywords

CD71 antigen; Cytogenetic damage; DNA damage; Micronuclei; Reticulocytes

Indexed keywords

ACRIDINE ORANGE; CD71 ANTIGEN; FLUORESCENT DYE; FLUOROURACIL; MONOCLONAL ANTIBODY; PROPIDIUM IODIDE; SODIUM CHLORIDE;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BLOOD ANALYSIS; BLOOD SAMPLING; CELL DENSITY; CELL LABELING; COLOR; CONTROLLED STUDY; CYTOGENETICS; DNA DAMAGE; DRUG CYTOTOXICITY; DRUG EFFECT; EVALUATION; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; GENOTOXICITY; HUMAN; HUMAN CELL; MAJOR CLINICAL STUDY; MALE; MICRONUCLEUS; MICRONUCLEUS TEST; MOUSE; MOUSE STRAIN; NONHUMAN; RAT; RAT STRAIN; REPRODUCIBILITY; RETICULOCYTE; SCORING SYSTEM; SENSITIVITY AND SPECIFICITY; TECHNIQUE; THROMBOCYTE AGGREGATION; VEIN PUNCTURE;

ADULT; ANIMALS; ANTIGENS, CD; ANTIGENS, DIFFERENTIATION, B-LYMPHOCYTE; ANTIMETABOLITES, ANTINEOPLASTIC; CHROMOSOME ABERRATIONS; CYTOGENETIC ANALYSIS; DNA DAMAGE; FEMALE; FLOW CYTOMETRY; FLUOROURACIL; HUMANS; MALE; MICE; MICRONUCLEUS TESTS; MIDDLE AGED; NEOPLASMS; PROPIDIUM; RATS; RATS, SPRAGUE-DAWLEY; RECEPTORS, TRANSFERRIN; RETICULOCYTES;

RODENTIA;

EID: 19944434262     PISSN: 08936692     EISSN: None     Source Type: Journal    
DOI: 10.1002/em.20075     Document Type: Article

References (21)

1. Abramsson-Zetterberg L, Grawe J, Zetterberg G. 1999. The micronucleus test in rat erythrocytes from bone marrow, spleen and peripheral blood: the response to low doses of ionizing radiation, cyclophosphamide and vincristine determined by flow cytometry. Mutat Res 423:113-124.
2. Abramsson-Zetterberg L, Zetterberg G, Bergqvist M, Grawe J. 2000. Human cytogenetic biomonitoring using flow-cytometric analysis of micronuclei in transferrin-positive immature peripheral blood reticulocytes. Environ Mol Mutagen 36:22-31.
3. Asanami S, Shimono K, Sawamoto O, Kurisu K, Uejima M. 1995. The suitability of rat peripheral blood in subchronic studies for the micronucleus assay. Mutat Res 347:73-78.
4. Dertinger SD, Torous DK, Tometsko K. 1996. Simple and reliable enumeration of micronucleated reticulocytes with a single-laser flow cytometer. Mutat Res 371:283-292.
5. Dertinger SD, Torous DK, Hall NE, Tometsko CR, Gasiewicz TA. 2000. Malaria-infected erythrocytes serve as biological standards to ensure reliable and consistent scoring of micronucleated erythrocytes by flow cytometry. Mutat Res 464:195-200.
6. Dertinger SD, Torous DK, Hall NE, Murante FG, Gleason SE, Miller RK, Tometsko CR. 2002. Enumeration of micronucleated CD71-positive human reticulocytes with a single-laser flow cytometer. Mutat Res 515:3-14.
7. Dertinger SD, Chen Y, Miller RK, Brewer KJ, Smudzin T, Torous DK, Hall NE, Olvany KA, Murante FG, Tometsko CR. 2003. Micronucleated CD71-positive reticulocytes: a blood-based endpoint of cytogenetic damage in humans. Mutat Res 542:77-87.
8. Gollapudi BB, Krishna G. 2000. Practical aspects of mutagenicity testing strategy: an industrial perspective. Mutat Res 455:21-28.
9. Hamada S, Yamasaki K, Nakanishi S, Omori T, Serikawa T, Hayashi M. 2001. Evaluation of the general suitability of the rat for the micronucleus assay: the effect of cyclophosphamide in 14 strains. Mutat Res 495:127-134.
10. Hayashi M, Sofuni T, Ishidate M Jr. 1983. An application of acridine orange fluorescent staining to the micronucleus test. Mutat Res 120:241-247.
11. Hayashi M, Kodama Y, Awogi T, Suzuki T, Asita AO, Sufuni T. 1992. The micronucleus assay using peripheral blood reticulocytes from mitomycin C- and cyclophosphamide-treated rats. Mutat Res 278: 209-213.
12. Hayashi M, MacGregor JT, Gatehouse DG, Adler I-D, Blakey DH, Dertinger SD, Krishna G, Morita T, Russo A, Sutou S. 2000. In vivo rodent erythrocyte micronucleus assay, aspects of protocol design including repeated treatments, integration with toxicity testing, and automated scoring - a report from the International Workshop on Genotoxicity Test Procedures (IWGTP). Environ Mol Mutagen 35:234-252.
13. Heddle J. 1973. A rapid in vivo test for chromosome damage. Mutat Res 18:187-190.
14. MacGregor J, Wehr C, Gould G. 1980. Clastogen-induced micronuclei in peripheral blood erythrocytes: the basis of an improved micronucleus test. Environ Mutagen 2:509-514.
15. Salamone MF, Mavournin KH. 1994. Bone marrow micronucleus assay: a review of the mouse stocks used and their published mean spontaneous micronucleus frequencies. Environ Mol Mutagen 23:239-273.
16. Schlegel R, MacGregor JT. 1984. The persistence of micronucleated erythrocytes in the peripheral circulation of normal and splenectomized Fischer 344 rats: implications for cytogenetic screening. Mutat Res 127:169-174.
17. Smith DF, MacGregor JT, Hiatt RA, Hooper NK, Wehr CM, Peters B, Goldman LR, Yuan LA, Smith PA, Becker CE. 1990. Micronucleated erythrocytes as an index of cytogenetic damage in humans: demographic and dietary factors associated with micronucleated erythrocytes in splenectomized subjects. Cancer Res 50:5049-5054.
18. Tometsko AM, Torous DK, Dertinger SD. 1993. Analysis of micronucleated cells by flow cytometry: I, achieving high resolution with a malaria model. Mutat Res 292:129-135.
19. Torous DK, Hall NE, Dertinger SD, Diehl MS, Illi-Love AH, Cederbrant K, Sandelin K, Bolcsfoldi G, Ferguson LR, Pearson A, Majeska JB, Tarca JP, Hewish DR, Doughty L, Fenech M, Weaver JL, Broud DD, Gatehouse DG, Hynes GM, Kwanyuen P, McLean J, McNamee JP, Parenteau M, Van Hoof V, Vanparys P, Lenarczyk M, Siennicka J, Litwinska B, Slowikowska MG, Harbach PR. Johnson CW, Zhao S, Aaron CS, Lynch AM, Marshall IC, Rodgers B, Tometsko CR. 2001. Flow cytometric enumeration of micronucleated reticulocytes: high transferability among 14 laboratories. Environ Mol Mutagen 38:59-68.
20. Torous DK, Hall NE, Murante FG, Gleason SE, Tometsko CR, Dertinger SD. 2003. Comparative scoring of micronucleated reticulocytes in rat peripheral blood by flow cytometry and microscopy. Tox Sci 74:309-314.
21. Wakata A, Miyamae Y, Sato S, Suzuki T, Morita T, Asano N, Awogi T, Kondo K, Hayashi M. 1998. Evaluation of the rat micronucleus test with bone marrow and peripheral blood: summary of the 9th collaborative study by CSGMT/JEMS.MMS. Environ Mol Mutagen 32:84-100.