Rapid detection of enterotoxigenic clostridium perfringens in meat samples using immunomagnetic separation polymerase chain reaction (IMS-PCR)

Journal of Agricultural and Food Chemistry

Volumn 58, Issue 12, 2010, Pages 7135-7140

  Yang, Zheng You ^a,b   Shim, Won Bo ^b   Kim, Kyeong Yeol ^b   Chung, Duck Hwa ^b  

^a SHANDONG AGRICULTURAL UNIVERSITY   (China)

^b Gyeongsang National University   (South Korea)

Author keywords

Enterotoxigenic Clostridium perfringens; Foodborne pathogen; Immunomagnetic separation PCR; Monoclonal antibody

Indexed keywords

ANIMAL; ARTICLE; CATTLE; CHICKEN; CLOSTRIDIUM PERFRINGENS; EVALUATION; GENETICS; IMMUNOMAGNETIC SEPARATION; ISOLATION AND PURIFICATION; MEAT; METHODOLOGY; POLYMERASE CHAIN REACTION; SWINE; ANALYSIS; BOVINAE; EVALUATION STUDY; PROCEDURES;

ANIMALS; CATTLE; CHICKENS; CLOSTRIDIUM PERFRINGENS; IMMUNOMAGNETIC SEPARATION; MEAT; POLYMERASE CHAIN REACTION; SWINE;

BACTERIA (MICROORGANISMS); CLOSTRIDIA; CLOSTRIDIUM PERFRINGENS; STAPHYLOCOCCUS AUREUS;

EID: 77953636864     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/jf1009654     Document Type: Article

References (37)

1. Brynestad, S.; Granum, P. E. Clostridium perfringens and foodborne infections Int. J. Food Microbiol. 2002, 74, 195-202
2. McClane, B. A.; Uzal, F. A.; Miyakawa, M. F.; Lyerly, D.; Wilkins, T. The enterotoxic Clostridia. In The Prokaryotes; Dworkin, M.; Falkow, S.; Rosenburg, E.; Schlcifer, K. H.; Stackebrandt, E., Eds.; Springer-Verlag: New York, 2006; Vol. 4, pp 698 -752.
3. Petit, L.; Gilbert, M.; Popoff, M. R. Clostridium perfringens: Toxinotype and genotype Trends Microbiol. 1999, 7, 104-110
4. Lin, Y. T.; Labbe, R. L. Enterotoxigenicity and genetic relatedness of Clostridium perfringens isolates from retail foods in the United States Appl. Environ. Microbiol. 2003, 69, 1642-1646
5. Centers for Disease Control and Prevention. Surveillance for Foodborne-Disease Outbreaks -U.S. 1998-1992. Centers for Disease Control and Prevention, Atlanta, GA, 1996.
6. Centers for Disease Control and Prevention. Surveillance for Foodborne-Disease Outbreaks -U.S. 1993-1997. Centers for Disease Control and Prevention, Atlanta, GA, 2000.
7. Abubakar, I.; Irvine, L.; Aldus, C. F.; Wyatt, G. M.; Fordham, R.; Schelenz, S.; Shepstone, L.; Howe, A.; Peck, M. P.; Hunter, R. A systematic review of the clinical, public health and cost-effectiveness of rapid diagnostic tests for the detection and identification of bacterial intestinal pathogens in faeces and food Health Technol. Assess. 2007, 11, 1-216
8. Adak, G. K.; Long, S. M.; OBrien, S. J. Trends in indigenous foodborne disease and deaths England and Wales: 1992 to 2000 Gut 2002, 51, 832-841
9. Dalton, C. B.; Gregory, J.; Kirk, M. D; Stafford, R. J.; Givney, R.; Kraa, E.; Gould, D. Foodborne disease outbreaks in Australia, 1995 to 2000 Commun. Dis. Intell. 2004, 28, 211-224
10. McClane, B. C. Clostridium perfringens. In Food Microbiology Fundamentals and Frontiers; Doyle, M. P.; Beuchat, L. R.; Montville, T. J., Eds.; ASM Press: Washington, D.C., 1997; pp 305 -326.
11. Johnson, S.; Gerding, D. Enterotoxemic infections. In The Clostridia: Molecular Biology and Pathogenesis; Rood, J.; McClane, B. A.; Songer, J. G.; Titball, R. W., Eds.; Academic Press: London, U.K., 1997; pp 117 -140.
12. Daube, G.; Simon, P.; Limbourg, B.; Manteca, C.; Mainil, J.; Kaeckenbeeck, A. Hybridization of 2,659 Clostridium perfringens isolates with gene probes for seven toxins (α, β, e, μ and enterotoxin) and for sialidase Am. J. Vet. Res. 1996, 57, 496-501
13. Kokai-Kun, J. F.; Songer, J. G.; Czeczulin, J. R.; Chen, F.; McClane, B. A. Comparison of western immunoblots and gene detection assays for identification of potentially enterotoxigenic isolates of Clostridium perfringens J. Clin. Microbiol. 1994, 32, 2533-2539
14. Cudjoe, K. S.; Thorsen, L. I.; Sorensen, T.; Reseland, J.; Olsvik, O.; Granum, P. E. Detection of Clostridium perfringens type A enterotoxin in faecal and food samples using immunomagnetic separation (IMS)-ELISA Int. J. Food Microbiol. 1991, 12, 313-322
15. Eisgruber, H.; Wiedmann, M.; Stoole, A. Use of plasmid profiling as a typing method for epidemiologically related Clostridium perfringens isolates from food poisoning cases and outbreaks Lett. Appl. Microbiol. 1995, 20, 290-294
16. Schalch, B.; Sperner, B.; Eisgruber, H.; Stoole, A. Molecular methods for the analysis of Clostridium perfringens relevant to food hygiene FEMS Immunol. Med. Microbiol. 1999, 24, 281-286
17. Mahony, D. E.; Ahmed, R.; Jackson, S. G. Multiple typing techniques applied to a Clostridium perfringens food poisoning outbreak J. Appl. Bacteriol. 1992, 72, 309-314
18. Shimizu, S.; Ootsubo, M.; Kubosawa, Y. J.; Fuchizawa, I.; Kawai, Y.; Yamazaki, K. Fluorescent in situ hybridization combination with filter cultivation (FISHFC) method for specific detection and enumeration of viable Clostridium perfringens strains Food Microbiol. 2009, 26, 425-431
19. Wise, M. G.; Siragusa, G. R. Quantitative detection of Clostridium perfringens in the broiler fowl gastrointestinal tract by real-time PCR Appl. Environ. Microbiol. 2005, 71, 3911-3916
20. Tansuphasiri, U.; Chanyasanha, C.; Cheaochantanakij, N. An enrichment broth culture-duplex PCR combination assay for the rapid detection of enterotoxigenic Clostridium perfringens in fecal specimens Southeast Asian J. Trop. Med. Public Health 2005, 36 (5) 1229-1238
21. Augustynowicz, E.; Gzyl, A.; Slusarczyk, J. Detection of enterotoxigenic Clostridium perfringens with a duplex PCR J. Med. Microbiol. 2002, 51 (2) 169-172
22. Magliulo, M.; Simoni, P.; Guardigli, M.; Michelini, E.; Luciani, M.; Lelli, R.; Roda, A. A rapid multiplexed chemiluminescent immunoassay for the detection of Escherichia coli O157:H7, Yersinia enterocolitica, Salmonella typhimurium, and Listeria monocytogenes pathogen bacteria J. Agric. Food Chem. 2007, 55 (13) 4933-4939
23. Kim, S.; Labbe, R. G.; Ryu, S. Inhibitory effects of collagen on the PCR for detection of Clostridium perfringens Appl. Environ. Microbiol. 2000, 66, 1213-1215
24. Hudson, J. A.; Lake, R. J.; Savill, M. G.; Scholes, P.; McCormick, R. E. Rapid detection of Listeria monocytogenes in ham samples using immunomagnetic separation followed by polymerase chain reaction J. Appl. Microbiol. 2001, 90, 614-621
25. Alarcón, B.; García-Cañas, V.; Cifuentes, A.; González, R.; Aznar, R. Simultaneous and sensitive detection of three foodborne pathogens by multiplex PCR, capillary gel electrophoresis, and laser-induced fluorescence J. Agric. Food Chem. 2004, 52 (23) 7180-7186
26. Fitzmaurice, J.; Duffy, G.; Kilbride, B.; Sheridan, J. J.; Carroll, C.; Maher, M. Comparison of a membrane surface adhesion recovery method with an IMS method for use in a polymerase chain reaction method to detect Escherichia coli O157:H7 in minced beef J. Microbiol. Methods 2004, 59, 243-252
27. Yang, Z.-Y.; Kolosava, A. Y.; Shim, W.-B.; Chung, D.-H. Development of monoclonal antibodies against pirimiphos-methyl and their application to IC-ELISA J. Agric. Food Chem. 2006, 54 (13) 4551-4556
28. Kristian, M; Ahrens, P. Comparison of toxicity neutralization-, ELISA-and PCR tests for typing of Clostridium perfringens and detection of the enterotoxin gene by PCR Anaerobe 1996, 2, 103-110
29. Meer, R. R.; Songer, J. G. Multiplex polymerase chain reaction assay for genotyping Clostridium perfringens Am. J. Vet. Res. 1997, 58, 702-705
30. Rhodehamel, E. J.; Harmon, S. M. Clostridium perfringens. In Bacteriological Analytical Manual, 8th ed.; U.S. Food and Drug Administration, Association of Official Analytical Chemists (AOAC) International: Gaithersburg, MD, 2001; Chapter 16, Revision A.
31. Lin, Y. T.; Labbe, R. Enterotoxigenicity and genetic relatedness of Clostridium perfringens isolates from retail foods in the united states Appl. Environ. Microbiol. 2003, 69, 1642-1646
32. Swanimathan, B.; Feng, P. Rapid detection of food-borne pathogenic bacteria Annu. Rev. Microbiol. 1994, 58, 2292-2295
33. Kim, S.; Labbe, R. G.; Ryu, S. Inhibitory effects of collagen on the PCR for detection of Clostridium perfringens Appl. Environ. Microbiol. 2000, 66, 1213-1215
34. Ueda, S.; Maruyama, T.; Kuwabara, Y. Detection of Listeria monocytogenes from food samples by PCR after IMS-plating Biocontrol Sci. 2006, 11, 129-134
35. Bhunia, A. K.; Ball, P. H.; Fuad, A. T.; Kurz, B. W.; Emerson, J. W.; Johnson, M. G. Development and characterization of a monoclonal antibody specific for Listeria monocytogenes and Listeria innocua Appl. Environ. Microbiol. 1991, 59, 3176-3184
36. McCout, M. T.; Finlay, D. A.; Laird, C.; Smyth, J. A.; Bell, C.; Ball, H. J. Sandwich ELISA detection of Clostridium perfringens cells and α-toxin from field cases of necrotic enteritis of poultry Vet. Microbiol. 2005, 106, 259-264
37. Miwa, N.; Nishina, T.; Kubo, S.; Honda, H. Most probable numbers of enterotoxigenic C. perfringens in intestinal contents of domestic livestock detected by nested PCR J. Vet. Med. Sci. 1997, 59, 557-560