Continuous processing of recombinant proteins: Integration of inclusion body solubilization and refolding using simulated moving bed size exclusion chromatography with buffer recycling

Journal of Chromatography A

Volumn 1319, Issue , 2013, Pages 107-117

  Wellhoefer, Martin ^a   Sprinzl, Wolfgang ^a   Hahn, Rainer ^a,b   Jungbauer, Alois ^a,b  

^a AUSTRIAN CENTRE OF INDUSTRIAL BIOTECHNOLOGY   (Austria)

^b UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

Author keywords

Autoprotease fusion protein; Buffer recycling; Continuous refolding; Inclusion body; Simulated moving bed

Indexed keywords

CONTINUOUS PROCESSING; FUSION PROTEINS; HIGH CELL DENSITY FERMENTATIONS; INCLUSION BODIES; REFOLDING; SIMULATED MOVING BED; SIMULATED MOVING BED SYSTEMS; TANGENTIAL FLOW FILTRATIONS;

CHROMATOGRAPHY; DISSOLUTION; PRODUCTIVITY; PROTEINS; UREA;

RECYCLING;

RECOMBINANT PROTEIN; SODIUM HYDROXIDE;

ANALYTIC METHOD; ARTICLE; BUFFER RECYCLING; CELL DENSITY; CONCENTRATION (PARAMETERS); CONTINUOUS PROCESS; DILUTION; DISSOLUTION; FERMENTATION TECHNIQUE; FILTRATION; LIQUID CHROMATOGRAPHY; PRIORITY JOURNAL; PROCESS DEVELOPMENT; PROTEIN DEGRADATION; PROTEIN INTERACTION; PROTEIN REFOLDING; SEPARATION TECHNIQUE; SIMULATED MOVING BED SIZE EXCLUSION CHROMATOGRAPHY;

AUTOPROTEASE FUSION PROTEIN; BUFFER RECYCLING; CONTINUOUS REFOLDING; INCLUSION BODY; SIMULATED MOVING BED;

BUFFERS; CHROMATOGRAPHY, GEL; PROTEIN FOLDING; RECOMBINANT PROTEINS; SOLUBILITY;

EID: 84887612059     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2013.10.039     Document Type: Article

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