Fourier transform infrared spectroscopy analysis of the conformational quality of recombinant proteins within inclusionbodies

Biotechnology Journal

Volumn 3, Issue 2, 2008, Pages 193-201

  Doglia, Silvia Maria ^a   Ami, Diletta ^a   Natalello, Antonino ^a   Gatti Lafranconi, Pietro ^a   Lotti, Marina ^a  

^a UNIVERSITY OF MILANO BICOCCA   (Italy)

Author keywords

Fourier transform infrared spectroscopy; Inclusion bodies; Recombinant proteins; Solubility

Indexed keywords

INCLUSION BODY; POLYPETIDES; RECOMBINANT PROTEIN;

AGGLOMERATION; FOURIER TRANSFORM INFRARED SPECTROSCOPY; GENETIC ENGINEERING; MOLECULAR STRUCTURE; SOLUBILITY;

PEPTIDES;

BETA GALACTOSIDASE; CHAPERONE; CHAPERONIN; ENDOPEPTIDASE CLP; ENDOPEPTIDASE LA; POLYPEPTIDE; PROTEIN CLPB; PROTEIN DNAK; RECOMBINANT PROTEIN;

ANALYTIC METHOD; BACTERIAL CELL; BIOTECHNOLOGY; CELL INCLUSION; CONTROLLED STUDY; ESCHERICHIA COLI; GENE OVEREXPRESSION; IN VIVO STUDY; INFRARED SPECTROSCOPY; MOLECULAR MECHANICS; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN ANALYSIS; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; PURIFICATION; REVIEW; STRUCTURE ANALYSIS; TEMPERATURE; CHEMISTRY; METABOLISM; METHODOLOGY; SOLUBILITY;

BACTERIA (MICROORGANISMS);

ELECTROPHORESIS, POLYACRYLAMIDE GEL; INCLUSION BODIES; PROTEIN CONFORMATION; PROTEIN FOLDING; RECOMBINANT PROTEINS; SOLUBILITY; SPECTROSCOPY, FOURIER TRANSFORM INFRARED;

EID: 41049088527     PISSN: 18606768     EISSN: None     Source Type: Journal    
DOI: 10.1002/biot.200700238     Document Type: Review

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