Recombinant expression and biochemical characterisation of two alanyl aminopeptidases of Trypanosoma congolense

Experimental Parasitology

Volumn 135, Issue 4, 2013, Pages 675-684

  Pillay, Davita ^a,d   Boulangé, Alain F V ^a,b,c   Coustou, Virginie ^d   Baltz, Théo ^d   Coetzer, Theresa H T ^a  

^a UNIVERSITY OF KWAZULU NATAL   (South Africa)

^b CIRAD   (France)

^c Institut de recherche pour le développement   (France)

^d UNIVERSITÉ DE BORDEAUX   (France)

Author keywords

M1 type aminopeptidase; Protozoan parasite; RNAi; Trypanosoma congolense

Indexed keywords

AMASTATIN; ANTIGEN; BESTATIN; MICROSOMAL AMINOPEPTIDASE; PARASITE ANTIGEN; PUROMYCIN; RECOMBINANT PROTEIN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CYTOPLASM; DOWN REGULATION; ENZYME ANALYSIS; ESCHERICHIA COLI; GROWTH RATE; IMMUNOPRECIPITATION; IN VITRO STUDY; MOLECULAR CLONING; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PARASITE IDENTIFICATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; RNA INTERFERENCE; SENSITIVITY ANALYSIS; TRYPANOSOMA CONGOLENSE;

4′,6-DIAMIDINO-2-PHENYLINDOLE; 4-(2-AMINOETHYL)BENZENESULFONYL FLUORIDE; AAT; ACETATE-MES-TRIS; AEBSF; AFRICAN ANIMAL TRYPANOSOMOSIS; AMC; AMINO PEPTIDASE; AMINOMETHYLCOUMARIN; AMT; AP; BENZYLOXYCARBONYL; BLOODSTREAM FORM; BSF; DAPI; DIMETHYL SULFOXIDE; DITHIOTHREITOL; DMSO; DTT; E-64; L-TRANS-EPOXYSUCCINYL-LEUCYLAMIDO(4-GUANIDINO)BUTANE; LC-MS/MS; LIQUID CHROMATOGRAPHY COUPLED TO TANDEM MASS SPECTROMETRY; M-MALEIMIDOBENZOYL-N-HYDROXYSUCCINIMIDE ESTER; M1 TYPE AMINOPEPTIDASE; MBS; PBS; PHOSPHATE BUFFERED SALINE, PH 7.2; PHOSPHATE-BUFFERED SALINE CONTAINING GLUCOSE; PROTOZOAN PARASITE; PSG; RNAI; ROOM TEMPERATURE; RT; TRYPANOSOMA CONGOLENSE; VARIABLE SURFACE GLYCOPROTEIN; VSG; Z;

AMINO ACID SEQUENCE; ANIMALS; ANTIGENS, CD13; ANTIGENS, PROTOZOAN; BLOTTING, WESTERN; CATIONS, DIVALENT; CATTLE; CHROMATOGRAPHY, LIQUID; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HYDROGEN-ION CONCENTRATION; IMMUNOPRECIPITATION; METALS; MICE; PROTEIN REFOLDING; RECOMBINANT PROTEINS; RNA, PROTOZOAN; TANDEM MASS SPECTROMETRY; TRYPANOSOMA CONGOLENSE;

EID: 84887376208     PISSN: 00144894     EISSN: 10902449     Source Type: Journal    
DOI: 10.1016/j.exppara.2013.10.005     Document Type: Article

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