Charge heterogeneity profiling of monoclonal antibodies using low ionic strength ion-exchange chromatography and well-controlled pH gradients on monolithic columns

Journal of Chromatography A

Volumn 1317, Issue , 2013, Pages 148-154

  Talebi, Mohammad ^a   Nordborg, Anna ^a   Gaspar, Andras ^a   Lacher, Nathan A ^b   Wang, Qian ^b   He, Xiaoping Z ^b   Haddad, Paul R ^a   Hilder, Emily F ^a  

^a UNIVERSITY OF TASMANIA   (Australia)

^b PFIZER INC   (United States)

Author keywords

Imaged capillary isoelectric focussing; Ion exchange; Liquid chromatography mass spectrometry; Monoclonal antibodies; PH gradient; Polymer monolith

Indexed keywords

CAPILLARY ISOELECTRIC FOCUSING; ELECTROSPRAY IONISATION MASS SPECTROMETRIES; HIGH-RESOLUTION SEPARATIONS; ISOELECTRIC FOCUSSING; LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY; MONOCLONAL ANTIBODIES (MAB); PH GRADIENTS; POLYMER MONOLITHS;

CHROMATOGRAPHY; ELECTROPHORESIS; ION EXCHANGE; IONIC STRENGTH; MASS SPECTROMETRY; PH; PROTEINS; SEPARATION; SURFACE PLASMON RESONANCE;

MONOCLONAL ANTIBODIES;

BUFFER; MONOCLONAL ANTIBODY; POLYMER; RECOMBINANT PROTEIN;

ARTICLE; CAPILLARY ISOELECTRIC FOCUSING; CHEMICAL PARAMETERS; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DONNAN POTENTIAL EFFECT; DRUG MANUFACTURE; DRUG STORAGE; ELECTROPHORETIC MOBILITY; ELECTROSPRAY IONISATION MASS SPECTROMETRY; IMAGED CAPILLARY ISOELECTRIC FOCUSING; ION EXCHANGE CHROMATOGRAPHY; IONIC STRENGTH; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PH; PRIORITY JOURNAL; PROTEIN ANALYSIS; STATIC ELECTRICITY; SURFACE PROPERTY; CHEMISTRY; DEVICES; ISOELECTRIC FOCUSING; ISOLATION AND PURIFICATION; OSMOLARITY; PROCEDURES;

ANTIBODIES, MONOCLONAL; BUFFERS; CHROMATOGRAPHY, ION EXCHANGE; HYDROGEN-ION CONCENTRATION; ISOELECTRIC FOCUSING; MASS SPECTROMETRY; OSMOLAR CONCENTRATION; POLYMERS; RECOMBINANT PROTEINS;

EID: 84887210834     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2013.08.061     Document Type: Article

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