Characterization of monoclonal antibodies using polymeric cation exchange monoliths in combination with salt and pH gradients

Journal of Separation Science

Volumn 32, Issue 15-16, 2009, Pages 2668-2673

  Nordborg, Anna ^a   Zhang, Bo ^b   He, Xiaoping Z ^b   Hilder, Emily F ^a   Haddad, Paul R ^a  

^a UNIVERSITY OF TASMANIA   (Australia)

^b PFIZER GLOBAL RESEARCH AND DEVELOPMENT   (United States)

Author keywords

Antibodies; Liquid chromatography; Monoclonal; Monolith; Protein

Indexed keywords

BEST CHOICE; BUFFER PH; CATION EXCHANGERS; CATION EXCHANGES; CHARGE HETEROGENEITY; ELUTION PROFILES; MONOCLONAL; MONOLITHIC COLUMNS; PH GRADIENTS; POLYMERIC CATION; POLYMETHACRYLATES; SALT GRADIENT;

CHROMATOGRAPHIC ANALYSIS; ION EXCHANGERS; LIQUIDS; MONOCLONAL ANTIBODIES; MONOLITHIC INTEGRATED CIRCUITS; PH EFFECTS; POSITIVE IONS; PROTEINS;

LIQUID CHROMATOGRAPHY;

MONOCLONAL ANTIBODY; POLYMETHACRYLIC ACID DERIVATIVE; SODIUM CHLORIDE; INORGANIC SALT; ION EXCHANGE RESIN;

ARTICLE; CAPILLARY CHROMATOGRAPHY; CATION EXCHANGE; CHEMICAL INTERACTION; ELUTION; HYDROPHOBICITY; ION EXCHANGE CHROMATOGRAPHY; PH; POLYMERIZATION; PRIORITY JOURNAL; PURIFICATION; SEPARATION TECHNIQUE; ANIMAL; CHEMISTRY; EVALUATION; HUMAN; INSTRUMENTATION; ISOLATION AND PURIFICATION; METHODOLOGY;

ANIMALS; ANTIBODIES, MONOCLONAL; CHROMATOGRAPHY, ION EXCHANGE; HUMANS; HYDROGEN-ION CONCENTRATION; ION EXCHANGE RESINS; SALTS;

EID: 70350488740     PISSN: 16159306     EISSN: 16159314     Source Type: Journal    
DOI: 10.1002/jssc.200900283     Document Type: Article

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