Replacement of the catalytic nucleophile aspartyl residue of dextran glucosidase by cysteine sulfinate enhances transglycosylation activity

Journal of Biological Chemistry

Volumn 288, Issue 44, 2013, Pages 31670-31677

  Saburi, Wataru ^a   Kobayashi, Momoko ^a   Mori, Haruhide ^a   Okuyama, Masayuki ^a   Kimura, Atsuo ^a  

^a HOKKAIDO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYTIC NUCLEOPHILES; HYDROLYTIC ACTIVITIES; ISOMALTO-OLIGOSACCHARIDES; STREPTOCOCCUS MUTANS; SUBSTRATE SPECIFICITY; TRANS GLYCOSYLATION ACTIVITY; TRANSGLUCOSYLATION; TRANSGLYCOSYLATION;

AMINO ACIDS; DEXTRAN; GLYCOSYLATION;

NUCLEOPHILES;

ASPARTIC ACID; CYSTEINE; CYSTEINE SULFINIC ACID; DEXTRANASE; NUCLEOPHILE;

AMINO ACID ANALYSIS; AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; CHEMICAL STRUCTURE; CONTROLLED STUDY; ENZYME ASSAY; GLYCOSYLATION; HYDROLYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATION; PEPTIDE MAPPING; PRIORITY JOURNAL; RESIDUE ANALYSIS; STREPTOCOCCUS MUTANS; WILD TYPE;

CATALYTIC NUCLEOPHILE; CYSTEINE SULFINATE; DEXTRAN GLUCOSIDASE; ENZYME CATALYSIS; ENZYME KINETICS; GLYCOSIDE HYDROLASE FAMILY 13; GLYCOSIDE HYDROLASES; PROTEIN ENGINEERING; SITE-SPECIFIC MUTAGENESIS; TRANSGLYCOSYLATION;

AMINO ACID SUBSTITUTION; ASPARTIC ACID; BACTERIAL PROTEINS; CATALYSIS; CYSTEINE; GLUCOSIDASES; GLYCOSYLATION; MUTATION, MISSENSE; STREPTOCOCCUS MUTANS;

EID: 84887062130     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M113.491449     Document Type: Article

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