Trypanosoma cruzi trans-sialidase operates through a covalent sialyl-enzyme intermediate: Tyrosine is the catalytic nucleophile

Journal of the American Chemical Society

Volumn 125, Issue 25, 2003, Pages 7532-7533

  Watts, Andrew G ^a   Damager, Iben ^a   Amaya, Maria L ^b   Buschiazzo, Alejandro ^b   Alzari, Pedro ^b   Frasch, Alberto C ^c   Withers, Stephen G ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b INSTITUT PASTEUR   (France)

^c UNIVERSIDAD NACIONAL DE SAN MARTÍN   (Argentina)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME; GLUTAMIC ACID; GLYCOSIDASE; GLYCOSYLPHOSPHATIDYLINOSITOL; MEMBRANE PROTEIN; NEURAMINIC ACID; SIALIC ACID DERIVATIVE; SIALIDASE; TYROSINE;

ARTICLE; BIOSYNTHESIS; CATALYSIS; CHAGAS DISEASE; CHEMICAL REACTION; MASS SPECTROMETRY; PARASITISM; TRYPANOSOMA CRUZI;

ANIMALS; CATALYSIS; ENZYME ACTIVATION; GLYCOPROTEINS; KINETICS; NEURAMINIDASE; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TRYPANOSOMA CRUZI; TYROSINE;

EID: 0038546856     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja0344967     Document Type: Article

References (16)

1. Parodi, A. J.; Pollevick, G. D.; Mautner, M.; Buschiazzo, A.; Sanchez, D.; Frasch, A. C. C. EMBO J. 1992, 11, 1705.
2. Trans-sialidase does possess hydrolytic activity in the absence of a lactose acceptor. Montagna, G.; Cremona, M. L.; Paris, G.; Amaya, M. F.; Buschiazzo, A.; Alzari, P. M.; Frasch, A. C. C. Eur. J. Biochem. 2002, 269, 2941.
3. Frasch, A. C. C. Parasitol. Today 2000, 16, 282.
4. Amaya, M. F.; Buschiazzo, A.; Nguyen, T.; Alzari, P. M. J. Mol. Biol. 2003, 325, 773.
5. Buschiazzo, A.; Amaya, M. F.; Cremona, M. L.; Frasch, A. C.; Alzari, P. M. Mol. Cell 2002, 10, 757.
6. Horenstein, B. A.; Yang, J.; Bruner, M. Nukleonika 2002, 47, S25.
7. Ashwell, M.; Guo, X.; Sinnott, M. L. J. Am. Chem. Soc. 1992, 114, 10158.
8. Scudder, P.; Doom, J. P.; Chuenkova, M.; Manger, I. D.; Pereira, M. E. A. J. Biol. Chem. 1993, 268, 9886.
9. Wicki, J.; Rose, D. R.; Withers, S. G. Methods Enzymol. 2002, 354, 84.
10. McCarter, J. D.; Withers, S. G. J. Am. Chem. Soc. 1996, 118, 241.
11. Zechel, D. L.; Withers, S. G. Acc. Chem. Res. 2000, 33, 11.
12. Synthesis to be reported elsewhere.
13. Paris, G.; Cremona, M. L.; Amaya, M. F.; Buschiazzo, A.; Giambiagi, S.; Frasch, A. C. C.; Alzari, P. M. Glycobiology 2001, 11, 305.
14. react, E-I accumulates and inactivation is observed.
15. T. cruzi contains over 100 genes encoding for trans-sialidase, of which about half are catalytically inactive and contain the mutation Y342H (ref 1).
16. Interestingly, the Glu to Asp mutant of Agrobacterium β-glucosidase underwent time-dependent inactivation in the presence of substrate via formation of a stable tyrosyl-glycoside. Gebler, J. C.; Trimbur, D. E.; Warren, R. A. J.; Aebersold, R.; Namchuck, M.; Withers, S. G. Biochemistry 1995, 34, 14547.