Trapping and characterization of the reaction intermediate in cyclodextrin glycosyltransferase by use of activated substrates and a mutant enzyme

Biochemistry

Volumn 36, Issue 32, 1997, Pages 9927-9934

  Mosi, Renee ^a   He, Shouming ^a   Uitdehaag, Joost ^b   Dijkstra, Bauke W ^b   Withers, Stephen G ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ACID; ASPARTIC ACID; BASE; CYCLOMALTODEXTRIN GLUCANOTRANSFERASE; ENZYME; FLUORIDE; HYDROXYL GROUP; OLIGOSACCHARIDE; STARCH;

ARTICLE; BACILLUS CIRCULANS; DEGRADATION; ENZYME SUBSTRATE; GLYCOSYLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; MUTANT; NONHUMAN; PRIORITY JOURNAL; TANDEM MASS SPECTROMETRY;

AMINO ACID SEQUENCE; BACILLUS; BINDING SITES; ENZYME ACTIVATION; FLUORIDES; GLUCOSYLTRANSFERASES; GLUTAMIC ACID; GLUTAMINE; KINETICS; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PEPTIDES; SUBSTRATE SPECIFICITY;

BACILLUS CIRCULANS; FELIS CATUS;

EID: 0030843867     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi970618u     Document Type: Article

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