Identification of Asp 549 as the catalytic nucleophile of glycogen-debranching enzyme via trapping of the glycosyl-enzyme intermediate

Biochemistry

Volumn 35, Issue 17, 1996, Pages 5458-5463

  Braun, Curtis ^a   Lindhorst, Thisbe ^a   Madsen, Neil B ^b   Withers, Stephen G ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b UNIVERSITY OF ALBERTA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

GLYCOGEN; GLYCOGEN DEBRANCHING ENZYME;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME MECHANISM; ENZYME SUBSTRATE COMPLEX; GLYCOGENOLYSIS; MUSCLE METABOLISM; NONHUMAN; PRIORITY JOURNAL; RABBIT; TANDEM MASS SPECTROMETRY;

AMINO ACID SEQUENCE; ANIMALS; ASPARTIC ACID; CARBOHYDRATE SEQUENCE; CATALYSIS; CONSENSUS SEQUENCE; GLYCOGEN; GLYCOGEN DEBRANCHING ENZYME SYSTEM; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; MUSCLES; RABBITS; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 0029865952     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi9526488     Document Type: Article

References (23)

1. Braun, C. (1995) Ph.D. Thesis, University of British Columbia, Cancouver, BC, Canada.
2. Braun, C., & Withers, S. G. (1995) Carbohydr. Res. 271, 113.
3. Brown, D. H., & Brown, B. I. (1966) Methods Enzymol. 8, 515.
4. Henderson, R. A., Michel, H., Sakaguchi, K., Shabanowitz, J., Appella, E., Hunt, D. F., & Engelhard, V. H. (1992) Science 255, 1264.
5. Hunt, D. F., Henderson, R. A., Shabanowitz, J., Sakaguchi, K., Michel, H., Sevilir, N., Cox, A. L., Appella, E., & Engelhard, V. H. (1992) Science 255, 1261.
6. Jespersen, H. M., MacGregor, E. A., Henrissat, B., Sierks, M. R., & Svensson, B. (1993) J. Protein Chem. 12, 791.
7. Lindhorst, T. K., Braun, C., & Withers, S. G. (1995) Carbohydr. Res. 268, 93.
8. Liu, L., de Castro, L., Takrama. J., Bilous, P. T., Vinayagamoorthy, T., Madsen, N. B., & Bleackley, C. R. (1993) Arch. Biochem. Biophys. 306, 232.
9. Liu, W., Madsen, N. B., Braun, C., & Withers, S. G. (1991) Biochemistry 30, 1419.
10. Matsuura, Y., Kusunoki, M., Harada, W., & Kakudo, M. (1984) J. Biochem. 95, 697.
11. McCarter, J. D., & Withers, S. G. (1994) Curr. Opin. Struct. Biol. 4, 361.
12. McCarter, J. D., Adam, M. J., Braun, C., Namchuk, M., Tull, D., & Withers, S. G. (1993) Carbohydr. Res., 249, 77.
13. Miao, S., McCarter, J. D., Grace, M., Grabowski, G., Aebersold, R., & Withers. S. G. (1994a) J. Biol. Chem. 269, 10975.
14. Miao, S., Ziser, L., Aebersold, R., & Withers, S. G. (1994b) Biochemistry 33, 7027.
15. Mooser, G., Hefta, S. A., Paxton, R. J., Shively, J. E., & Lee, T. D. (1991) J. Biol. Chem. 266, 8916.
16. Nelson, T. E., White, R. C., & Gillard, B. K. (1979) ACS Symp. Ser. 88, 131.
17. Sinnott, M. L. (1990) Chem. Rev. 90, 1171.
18. Svensson, B. (1994) Plant Mol. Biol. 25, 141.
19. Tabata, S., & Dohi, Y. (1992) Carbohydr. Res. 230, 179
20. Takrama, J., & Madsen, N. B. (1988) Biochemistry 27, 3308.
21. Tull, D., Miao, S., Withers, S. G., & Aebersold, R. (1995) Anal. Biochem. 224, 509.
22. Withers, S. G., & Aebersold, R. (1995) Protein Sci. 4, 361.
23. Withers, S. G., Rupitz., K., & Street, I. P. (1988) J. Biol. Chem. 263, 7929.