Reconstitution of R-spondin:LGR4:ZNRF3 adult stem cell growth factor signaling complexes with recombinant proteins produced in escherichia coli

Biochemistry

Volumn 52, Issue 41, 2013, Pages 7295-7304

  Moad, Heather E ^a   Pioszak, Augen A ^a  

^a UNIVERSITY OF OKLAHOMA COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

E3 UBIQUITIN LIGASES; ELECTROPHORETIC MOBILITY SHIFT ASSAY; EXTRACELLULAR DOMAINS; LEUCINE-RICH REPEATS; PROLIFERATIVE EFFECTS; PROTEIN PRODUCTION; REGENERATIVE MEDICINE; TIME-RESOLVED FLUORESCENCE;

AMINO ACIDS; ELECTROPHORETIC MOBILITY; ENERGY TRANSFER; ESCHERICHIA COLI; RECOMBINANT PROTEINS; SIGNALING; STEM CELLS;

CELL SIGNALING;

CYSTEINE; GLYCAN; GLYCOPROTEIN; GLYCOPROTEIN RSPO1; MEMBRANE RECEPTOR; MEMBRANE RECEPTOR LGR4; RECOMBINANT PROTEIN; UBIQUITIN PROTEIN LIGASE E3; UBIQUITIN PROTEIN LIGASE E3 ZNRF3; UNCLASSIFIED DRUG; WNT3A PROTEIN;

ADULT STEM CELL; ANIMAL CELL; ARTICLE; BINDING AFFINITY; COMPLEX FORMATION; CONTROLLED STUDY; ESCHERICHIA COLI; EXPRESSION VECTOR; FLUORESCENCE RESONANCE ENERGY TRANSFER; GEL MOBILITY SHIFT ASSAY; HUMAN; HUMAN CELL; MAMMAL CELL; MEMBRANE RECONSTITUTION; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN QUATERNARY STRUCTURE; RECEPTOR BINDING; SIGNAL TRANSDUCTION;

ADULT STEM CELLS; ESCHERICHIA COLI; GENE EXPRESSION; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, G-PROTEIN-COUPLED; RECOMBINANT PROTEINS; SIGNAL TRANSDUCTION; UBIQUITIN-PROTEIN LIGASES; WNT3 PROTEIN;

EID: 84885991667     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi401090h     Document Type: Article

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