Bacterial expression and purification of a heterodimeric adrenomedullin receptor extracellular domain complex using DsbC-assisted disulfide shuffling

Protein Expression and Purification

Volumn 88, Issue 1, 2013, Pages 107-113

  Hill, Heather E ^a   Pioszak, Augen A ^a  

^a UNIVERSITY OF OKLAHOMA COLLEGE OF MEDICINE   (United States)

Author keywords

Co receptor; G protein coupled receptor; Peptide hormone; Vasodilator

Indexed keywords

ADRENOMEDULLIN RECEPTOR; CALCITONIN RECEPTOR LIKE RECEPTOR; DISULFIDE; MULTIPROTEIN COMPLEX; RECEPTOR ACTIVITY MODIFYING PROTEIN 1; RECEPTOR ACTIVITY MODIFYING PROTEIN 2; RECEPTOR ACTIVITY MODIFYING PROTEIN 3; VASODILATOR AGENT;

AMINO ACID SEQUENCE; ARTICLE; BINDING SITE; BIOSYNTHESIS; CHEMISTRY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; PROTEIN BINDING; PROTEIN TERTIARY STRUCTURE; SIGNAL TRANSDUCTION;

AMINO ACID SEQUENCE; BINDING SITES; CALCITONIN RECEPTOR-LIKE PROTEIN; DISULFIDES; HUMANS; MULTIPROTEIN COMPLEXES; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RECEPTOR ACTIVITY-MODIFYING PROTEIN 1; RECEPTOR ACTIVITY-MODIFYING PROTEIN 2; RECEPTOR ACTIVITY-MODIFYING PROTEIN 3; RECEPTORS, ADRENOMEDULLIN; SIGNAL TRANSDUCTION; VASODILATOR AGENTS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 84872115959     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2012.11.019     Document Type: Article

References (31)

1. K. Kitamura, K. Kangawa, M. Kawamoto, Y. Ichiki, S. Nakamura, H. Matsuo, and T. Eto Adrenomedullin: a novel hypotensive peptide isolated from human pheochromocytoma Biochem. Biophys. Res. Commun. 192 1993 553 560 (Pubitemid 23227359)
2. S.D. Brain, and A.D. Grant Vascular actions of calcitonin gene-related peptide and adrenomedullin Physiol. Rev. 84 2004 903 934 (Pubitemid 38823770)
3. C. Gibbons, R. Dackor, W. Dunworth, K. Fritz-Six, and K.M. Caron Receptor activity-modifying proteins: RAMPing up adrenomedullin signaling Mol. Endocrinol. 21 2007 783 796 (Pubitemid 46984723)
4. K.M. Caron, and O. Smithies Extreme hydrops fetalis and cardiovascular abnormalities in mice lacking a functional adrenomedullin gene Proc. Natl. Acad. Sci. USA 98 2001 615 619 (Pubitemid 32105091)
5. S.A. Hamid, and G.F. Baxter A critical cytoprotective role of endogenous adrenomedullin in acute myocardial infarction J. Mol. Cell. Cardiol. 41 2006 360 363 (Pubitemid 44080224)
6. L.L. Nikitenko, S.B. Fox, S. Kehoe, M.C. Rees, and R. Bicknell Adrenomedullin and tumour angiogenesis Br. J. Cancer 94 2006 1 7 (Pubitemid 43088532)
7. L.M. McLatchie, N.J. Fraser, M.J. Main, A. Wise, J. Brown, N. Thompson, R. Solari, M.G. Lee, and S.M. Foord RAMPs regulate the transport and ligand specificity of the calcitonin-receptor-like receptor Nature 393 1998 333 339 (Pubitemid 28269699)
8. D.R. Poyner, P.M. Sexton, I. Marshall, D.M. Smith, R. Quirion, W. Born, R. Muff, J.A. Fischer, and S.M. Foord International Union of Pharmacology. XXXII. The mammalian calcitonin gene-related peptides, adrenomedullin, amylin, and calcitonin receptors Pharmacol. Rev. 54 2002 233 246 (Pubitemid 34575714)
9. B.W. Purdue, N. Tilakaratne, and P.M. Sexton Molecular pharmacology of the calcitonin receptor Recept. Channels 8 2002 243 255
10. G. Christopoulos, K.J. Perry, M. Morfis, N. Tilakaratne, Y. Gao, N.J. Fraser, M.J. Main, S.M. Foord, and P.M. Sexton Multiple amylin receptors arise from receptor activity-modifying protein interaction with the calcitonin receptor gene product Mol. Pharmacol. 56 1999 235 242 (Pubitemid 29309833)
11. R. Muff, N. Buhlmann, J.A. Fischer, and W. Born An amylin receptor is revealed following co-transfection of a calcitonin receptor with receptor activity modifying proteins-1 or -3 Endocrinology 140 1999 2924 2927 (Pubitemid 32245175)
12. N. Tilakaratne, G. Christopoulos, E.T. Zumpe, S.M. Foord, and P.M. Sexton Amylin receptor phenotypes derived from human calcitonin receptor/RAMP coexpression exhibit pharmacological differences dependent on receptor isoform and host cell environment J. Pharmacol. Exp. Ther. 294 2000 61 72 (Pubitemid 30434671)
13. C. Parthier, M. Kleinschmidt, P. Neumann, R. Rudolph, S. Manhart, D. Schlenzig, J. Fanghanel, J.U. Rahfeld, H.U. Demuth, and M.T. Stubbs Crystal structure of the incretin-bound extracellular domain of a G protein-coupled receptor Proc. Natl. Acad. Sci. USA 104 2007 13942 13947 (Pubitemid 350003317)
14. A.A. Pioszak, and H.E. Xu Molecular recognition of parathyroid hormone by its G protein-coupled receptor Proc. Natl. Acad. Sci. USA 105 2008 5034 5039 (Pubitemid 351738505)
15. S.R. Hoare Mechanisms of peptide and nonpeptide ligand binding to Class B G-protein-coupled receptors Drug Discov. Today 10 2005 417 427 (Pubitemid 40450272)
16. S. Kusano, M. Kukimoto-Niino, R. Akasaka, M. Toyama, T. Terada, M. Shirouzu, T. Shindo, and S. Yokoyama Crystal structure of the human receptor activity-modifying protein 1 extracellular domain Protein Sci. 17 2008 1907 1914
17. S. Kusano, M. Kukimoto-Niino, N. Hino, N. Ohsawa, K. Okuda, K. Sakamoto, M. Shirouzu, T. Shindo, and S. Yokoyama Structural basis for extracellular interactions between calcitonin receptor-like receptor and receptor activity-modifying protein 2 for adrenomedullin-specific binding Protein Sci. 21 2012 199 210
18. T.J. Fitzsimmons, X. Zhao, and S.A. Wank The extracellular domain of receptor activity-modifying protein 1 is sufficient for calcitonin receptor-like receptor function J. Biol. Chem. 278 2003 14313 14320 (Pubitemid 36799981)
19. L.M. Ittner, D. Koller, R. Muff, J.A. Fischer, and W. Born The N-terminal extracellular domain 23-60 of the calcitonin receptor-like receptor in chimeras with the parathyroid hormone receptor mediates association with receptor activity-modifying protein 1 Biochemistry 44 2005 5749 5754 (Pubitemid 40525111)
20. D. Koller, W. Born, K. Leuthauser, B. Fluhmann, R.A. McKinney, J.A. Fischer, and R. Muff The extreme N-terminus of the calcitonin-like receptor contributes to the selective interaction with adrenomedullin or calcitonin gene-related peptide FEBS Lett. 531 2002 464 468 (Pubitemid 35336070)
21. K. Kuwasako, K. Kitamura, K. Ito, T. Uemura, Y. Yanagita, J. Kato, T. Sakata, and T. Eto The seven amino acids of human RAMP2 (86) and RAMP3 (59) are critical for agonist binding to human adrenomedullin receptors J. Biol. Chem. 276 2001 49459 49465
22. T. Qi, G. Christopoulos, R.J. Bailey, A. Christopoulos, P.M. Sexton, and D.L. Hay Identification of N-terminal receptor activity-modifying protein residues important for calcitonin gene-related peptide, adrenomedullin, and amylin receptor function Mol. Pharmacol. 74 2008 1059 1071
23. M. Chauhan, K. Rajarathnam, and C. Yallampalli Role of the N-terminal domain of the calcitonin receptor-like receptor in ligand binding Biochemistry 44 2005 782 789 (Pubitemid 40105510)
24. C.M. Koth, N. Abdul-Manan, C.A. Lepre, P.J. Connolly, S. Yoo, A.K. Mohanty, J.A. Lippke, J. Zwahlen, J.T. Coll, J.D. Doran, M. Garcia-Guzman, and J.M. Moore Refolding and characterization of a soluble ectodomain complex of the calcitonin gene-related peptide receptor Biochemistry 49 2010 1862 1872
25. E. ter Haar, C.M. Koth, N. Abdul-Manan, L. Swenson, J.T. Coll, J.A. Lippke, C.A. Lepre, M. Garcia-Guzman, and J.M. Moore Crystal structure of the ectodomain complex of the CGRP receptor, a class-B GPCR, reveals the site of drug antagonism Structure 18 2010 1083 1093
26. S. Kumar, A. Pioszak, C. Zhang, K. Swaminathan, and H.E. Xu Crystal structure of the PAC1R extracellular domain unifies a consensus fold for hormone recognition by class B G-protein coupled receptors PloS one 6 2011 e19682
27. K. Pal, K. Swaminathan, H.E. Xu, and A.A. Pioszak Structural basis for hormone recognition by the Human CRFR2{alpha} G protein-coupled receptor J. Biol. Chem. 285 2010 40351 40361
28. A.A. Pioszak, K.G. Harikumar, N.R. Parker, L.J. Miller, and H.E. Xu Dimeric arrangement of the parathyroid hormone receptor and a structural mechanism for ligand-induced dissociation J. Biol. Chem. 285 2010 12435 12444
29. A.A. Pioszak, N.R. Parker, T.J. Gardella, and H.E. Xu Structural basis for parathyroid hormone-related protein binding to the parathyroid hormone receptor and design of conformation-selective peptides J. Biol. Chem. 284 2009 28382 28391
30. A.A. Pioszak, N.R. Parker, K. Suino-Powell, and H.E. Xu Molecular recognition of corticotropin-releasing factor by its G-protein-coupled receptor CRFR1 J. Biol. Chem. 283 2008 32900 32912
31. P.H. Bessette, F. Aslund, J. Beckwith, and G. Georgiou Efficient folding of proteins with multiple disulfide bonds in the Escherichia coli cytoplasm Proc. Natl. Acad. Sci. USA 96 1999 13703 13708