Epidermal growth factor receptor subunit locations determined in hydrated cells with environmental scanning electron microscopy

Scientific Reports

Volumn 3, Issue , 2013, Pages

  Peckys, Diana B ^a   Baudoin, Jean Pierre ^b,d   Eder, Magdalena ^a,c,d   Werner, Ulf ^a   De Jonge, Niels ^a,b  

^a INM LEIBNIZ INSTITUTE FOR NEW MATERIALS   (Germany)

^b VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c CARL ZEISS SMT AG   (Germany)

^d AIX MARSEILLE UNIVERSITY   (France)

Author keywords

[No Author keywords available]

Indexed keywords

EPIDERMAL GROWTH FACTOR; EPIDERMAL GROWTH FACTOR RECEPTOR; METAL NANOPARTICLE;

ANIMAL; ARTICLE; CELL LINE; CHEMICAL STRUCTURE; CHEMISTRY; CHLOROCEBUS AETHIOPS; COS 1 CELL LINE; HUMAN; METABOLISM; PROTEIN CONFORMATION; PROTEIN MULTIMERIZATION; PROTEIN SUBUNIT; PROTEIN TRANSPORT; SCANNING ELECTRON MICROSCOPY;

ANIMALS; CELL LINE; CERCOPITHECUS AETHIOPS; COS CELLS; EPIDERMAL GROWTH FACTOR; HUMANS; METAL NANOPARTICLES; MICROSCOPY, ELECTRON, SCANNING; MODELS, MOLECULAR; PROTEIN CONFORMATION; PROTEIN MULTIMERIZATION; PROTEIN SUBUNITS; PROTEIN TRANSPORT; RECEPTOR, EPIDERMAL GROWTH FACTOR;

EID: 84885942826     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep02626     Document Type: Article

References (38)

1. Normanno,N. et al. Epidermal growth factor receptor (EGFR) signaling in cancer. Gene 366, 2-16 (2006).
2. Arkhipov, A. et al. Architecture and membrane interactions of the EGF receptor. Cell 152, 557-569 (2013).
3. Robinson, C. V., Sali, A. & Baumeister, W. The molecular sociology of the cell. Nature 450, 973-982 (2007).
4. Pierson, J., Sani, M., Tomova, C., Godsave, S. & Peters, P. J. Toward visualization of nanomachines in their native cellular environment. Histochem. Cell Biol. 132, 253-262 (2009).
5. Hoenger, A. & Bouchet-Marquis, C. Cellular tomography. Adv. Protein Chem. Struct. Biol. 82, 67-90 (2011).
6. Lippincott-Schwartz, J. & Manley, S. Putting super-resolution fluorescence microscopy to work. Nat. Meth. 6, 21-23 (2009).
7. Hell, S. W. Far-field optical nanoscopy. Science 316, 1153-1158 (2007).
8. Bader, A. N., Hofman, E. G., Voortman, J., en Henegouwen, P. M. & Gerritsen, H. C. Homo-FRET imaging enables quantification of protein cluster sizes with subcellular resolution. Biophys. J. 97, 2613-2622 (2009).
9. Bogner, A., Thollet, G., Basset, D., Jouneau, P. H. & Gauthier, C. Wet STEM: A new development in environmental SEM for imaging nano-objects included in a liquid phase. Ultramicroscopy 104, 290-301 (2005).
10. de Jonge, N., Peckys, D. B., Kremers, G. J. & Piston, D. W. Electron microscopy of whole cells in liquid with nanometer resolution. Proc. Natl. Acad. Sci. 106, 2159-2164 (2009).
11. Ring, E. A., Peckys, D. B., Dukes, M. J., Baudoin, J. P. & de Jonge, N. Silicon nitride windows for electron microscopy of whole cells. J. Microsc. 243, 273-283 (2011).
12. Hermann, R.,Walther, P.&Muller, M. Immunogold labeling in scanning electron microscopy. Histochem. Cell Biol. 106, 31-39 (1996).
13. Lidke, D. S. et al. Quantum dot ligands provide new insights into erB/HER receptor-mediated signal transduction. Nat. Biotechnol. 22, 198-203 (2004).
14. Hayat, M. A. Principles and Techniques of Electron Microscopy: Biological Applications. 4th edn, 564 (Cambridge University Press, 2000).
15. Ester, M., Kriegel, H.-P., Sander, J. & Xu, X. in Second International Conference on Knowledge Discovery and Data Mining. 226-231 (AAAI Press).
16. Ariotti, N. et al. Epidermal growth factor receptor activation remodels the plasma membrane lipid environment to induce nanocluster formation. Mol. Cell Biol. 30, 3795-3804 (2010).
17. Yang, S. et al. Mapping ErbB receptors on breast cancer cell membranes during signal transduction. J. Cell Sci. 120, 2763-2773 (2007).
18. van Belzen, N. et al. Direct visualization and quantitative analysis of epidermal growth factor-induced receptor clustering. J. Cell Physiol. 134, 413-420 (1988).
19. Wiegant, F. A. et al. Epidermal growth factor receptors associated to cytoskeletal elements of epidermoid carcinoma (A431) cells. J. Cell Biol. 103, 87-94 (1986).
20. Hermann, R.,Walther, P.&Muller, M. Immunogold labeling in scanning electron microscopy. Histochem. Cell Biol. 106, 31-39 (1996).
21. Ogiso, H. et al. Crystal Structure of the Complex of Human Epidermal Growth Factor and Receptor Extracellular Domains. Cell 110, 775-787 (2002).
22. Lillemeier, B. F., Pfeiffer, J. R., Surviladze, Z.,Wilson, B. S. & Davis, M. M. Plasma membrane-associated proteins are clustered into islands attached to the cytoskeleton. Proc. Natl. Acad. Sci. 103, 18992-18997 (2006).
23. van Belzen, N. et al. Direct visualization and quantitative analysis of epidermal growth factor-induced receptor clustering. J. Cell. Physiol. 134, 413-420 (1988).
24. Yang, S. et al. Mapping ErbB receptors on breast cancer cell membranes during signal transduction. J. Cell Sci. 120, 2763-2773 (2007).
25. Cambi, A. & Lidke, D. S. Nanoscale membrane organization: where biochemistry meets advanced microscopy. ACS Chem. Biol. 7, 139-149 (2012).
26. Endres, N. F. et al. Conformational Coupling across the Plasma Membrane in Activation of the EGF Receptor. Cell 152, 543-556 (2013).
27. Keating, E., Nohe, A. & Petersen, N. O. Studies of distribution, location and dynamic properties of EGFR on the cell surface measured by image correlation spectroscopy. Eur. Biophys. J. 37, 469-481 (2008).
28. Shu, X. et al. A genetically encoded tag for correlated light and electron microscopy of intact cells, tissues, and organisms. PLoS Biol. 9, e1001041 (2011).
29. Elsaesser, A. et al. Quantification of nanoparticle uptake by cells using microscopical and analytical techniques. Nanomedicine 5, 1447-1457 (2010).
30. Tantra, R. & Shard, A. We need answers. Nat. Nanotechnol. 8, 71 (2013).
31. Tantra, R. & Knight, A. Cellular uptake and intracellular fate of engineered nanoparticles: a review on the application of imaging techniques. Nanotoxicol. 5, 381-392 (2011).
32. Hahn, M. A., Singh, A. K., Sharma, P., Brown, S. C. & Moudgil, B. M. Nanoparticles as contrast agents for in-vivo bioimaging: current status and future perspectives. Anal. Bioanal. Chem. 399, 3-27 (2011).
33. Davis, M. E., Chen, Z. G. & Shin, D. M. Nanoparticle therapeutics: an emerging treatment modality for cancer. Nat. Rev. Drug. Discov. 7, 771-782 (2008).
34. Simons, K. Engineering the future with cell biology. Mol. Biol. Cell. 21, 3822 (2010).
35. Kirk, S., Skepper, J. & Donald, A. M. Application of environmental scanning electron microscopy to determine biological surface structure. J. Microsc. 233, 205-224 (2009).
36. Stoyan, D. & Stoyan, H. Estimating pair correlation functions of planar cluster processes. Biom. J. 38, 259-271 (1996).
37. Ripley, B. D. The Second-Order Analysis of Stationary Point Processes. J. Appl. Probability 13, 255-266 (1976).
38. Yamada, I. &Rogerson, P. A. An Empirical Comparison of Edge Effect Correction Methods Applied to K-function Analysis. Geographical Analysis 35, 97-109 (2003).