Visualization of TCR Nanoclusters via Immunogold Labeling, Freeze-Etching, and Surface Replication

Methods in Cell Biology

Volumn 117, Issue , 2013, Pages 391-410

  Fiala, Gina J ^a,b   Rejas, María Teresa ^c   Schamel, Wolfgang W ^a,b   van Santen, Hisse M ^c  

^a UNIVERSITY OF FREIBURG   (Germany)

^b UNIVERSITY OF FREIBURG   (Germany)

^c UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

Author keywords

Cell surface proteins; Cell surface replicas; Immunogold labeling; Receptor clustering; T cell receptor; Transmission electron microscopy

Indexed keywords

ALUMINUM SILICATE; COLLOIDAL GOLD; LYMPHOCYTE ANTIGEN RECEPTOR; MICA; T LYMPHOCYTE RECEPTOR;

ARTICLE; B LYMPHOCYTE; CELL ADHESION; CELL LINE; CELL MEMBRANE; CELL SURFACE PROTEINS; CELL SURFACE REPLICAS; CHEMISTRY; FREEZE ETCHING; GENE EXPRESSION; GENETICS; HUMAN; IMMUNOGOLD LABELING; IMMUNOHISTOCHEMISTRY; METABOLISM; METHODOLOGY; PROTEIN MULTIMERIZATION; RECEPTOR CLUSTERING; STAINING; SURFACE PROPERTY; T LYMPHOCYTE; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE;

CELL SURFACE PROTEINS; CELL SURFACE REPLICAS; IMMUNOGOLD LABELING; RECEPTOR CLUSTERING; T CELL RECEPTOR; TRANSMISSION ELECTRON MICROSCOPY;

ALUMINUM SILICATES; B-LYMPHOCYTES; CELL ADHESION; CELL LINE; CELL MEMBRANE; FREEZE ETCHING; GENE EXPRESSION; GOLD COLLOID; HUMANS; IMMUNOHISTOCHEMISTRY; MICROSCOPY, ELECTRON, TRANSMISSION; PROTEIN MULTIMERIZATION; RECEPTORS, ANTIGEN, T-CELL; STAINING AND LABELING; SURFACE PROPERTIES; T-LYMPHOCYTES;

EID: 84885939579     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/B978-0-12-408143-7.00021-9     Document Type: Chapter

References (25)

1. Arechaga I., Swamy M., Abia D., Schamel W.A., Alarcon B., Valpuesta J.M. Structural characterization of the TCR complex by electron microscopy. International Immunology 2010, 22:897-903.
2. Boonstra J., van Bergen en Henegouwen P.M., van Belzen N., Rijken P.J., Verkleij A.J. Immunogold labelling in combination with cryoultramicrotomy, freeze-etching, and label-fracture. Journal of Microscopy 1991, 161:135-147.
3. Bray D., Levin M.D., Morton-Firth C.J. Receptor clustering as a cellular mechanism to control sensitivity. Nature 1998, 393:85-88.
4. Demotz S., Grey H.M., Sette A. The minimal number of class II MHC-antigen complexes needed for T cell activation. Science 1990, 249:1028-1030.
5. Fiala, G. J., Kaschek, D., Blumenthal, B., Reth, M., Timmer, J., & Schamel, W. W. A. Pre-clustering of the B cell antigen receptor demonstrated by mathematically extended electron microscopy. Frontiers in B cell Biology. Under Revision.
6. Grakoui A., Bromley S.K., Sumen C., Davis M.M., Shaw A.S., Allen P.M. The immunological synapse: A molecular machine controlling T cell activation. Science 1999, 285:221-227.
7. Harding C.V., Unanue E.R. Quantitation of antigen-presenting cell MHC class II/peptide complexes necessary for T-cell stimulation. Nature 1990, 346:574-576.
8. Irvine D.J., Purbhoo M.A., Krogsgaard M., Davis M.M. Direct observation of ligand recognition by T cells. Nature 2002, 419:845-849.
9. James J.R., McColl J., Oliveira M.I., Dunne P.D., Huang E., Jansson A., et al. The T cell receptor triggering apparatus is composed of monovalent or monomeric proteins. Journal of Biological Chemistry 2011, 286:31993-32001.
10. Kumar R., Ferez M., Swamy M., Arechaga I., Rejas M.T., Valpuesta J.M., et al. Increased sensitivity of antigen-experienced T cells through the enrichment of oligomeric T cell receptor complexes. Immunity 2011, 35:375-387.
11. Lillemeier B.F., Mortelmaier M.A., Forstner M.B., Huppa J.B., Groves J.T., Davis M.M. TCR and Lat are expressed on separate protein islands on T cell membranes and concatenate during activation. Nature Immunology 2010, 11:90-96.
12. Molnar E., Deswal S., Schamel W.W. Pre-clustered TCR complexes. FEBS Letters 2010, 584:4832-4837.
13. Molnar E., Swamy M., Holzer M., Beck-Garcia K., Worch R., Thiele C., et al. Cholesterol and sphingomyelin drive ligand-independent T-cell antigen receptor nanoclustering. Journal of Biological Chemistry 2012, 287:42664-42674.
14. Monks C.R., Freiberg B.A., Kupfer H., Sciaky N., Kupfer A. Three-dimensional segregation of supramolecular activation clusters in T cells. Nature 1998, 395:82-86.
15. Nermut, M. V. (1995). Manipulation of cell monolayers to reveal plasma membrane surfaces for freeze-drying and surface replication. In D.M. Shotton (Series Ed.) & N. J. Severs, & D. M. Shotton (Vol. Eds.), Rapid freezing, freeze fracture and deep etching (techniques in modern biomedical microscopy) (pp. 151-172). Wiley-Liss, Inc, New York.
16. Schamel W.W., Alarcon B. Organization of the resting TCR in nanoscale oligomers. Immunological Reviews 2013, 251:13-20.
17. Schamel W.W., Arechaga I., Risueno R.M., van Santen H.M., Cabezas P., Risco C., et al. Coexistence of multivalent and monovalent TCRs explains high sensitivity and wide range of response. The Journal of Experimental Medicine 2005, 202:493-503.
18. Severs, N. J. (1995). Freeze-fracture cytochemistry: An explanatory survey of methods. In D. M. Shotton (Series Ed.) & N. J. Severs, & D. M. Shotton (Vol. Eds.), Rapid freezing, freeze fracture and deep etching (techniques in modern biomedical microscopy) (pp. 173-208). Wiley-Liss, Inc, New York.
19. Severs N.J. Freeze-fracture electron microscopy. Nature Protocols 2007, 2:547-576.
20. Severs, N. J., Newman, T. M., & Shotton, D. M. (1995). A practical introduction to rapid freezing techniques. In D. M. Shotton (Series Ed.) & N. J. Severs, & D. M. Shotton (Vol. Eds.), Rapid freezing, freeze fracture and deep etching (techniques in modern biomedical microscopy) (pp. 31-49). Wiley-Liss, Inc, New York.
21. Sherman E., Barr V., Manley S., Patterson G., Balagopalan L., Akpan I., et al. Functional nanoscale organization of signaling molecules downstream of the T cell antigen receptor. Immunity 2011, 35:705-720.
22. van Belzen N., Rijken P.J., Hage W.J., de Laat S.W., Verkleij A.J., Boonstra J. Direct visualization and quantitative analysis of epidermal growth factor-induced receptor clustering. Journal of Cell Physiology 1988, 134:413-420.
23. Varma R., Campi G., Yokosuka T., Saito T., Dustin M.L. T cell receptor-proximal signals are sustained in peripheral microclusters and terminated in the central supramolecular activation cluster. Immunity 2006, 25:117-127.
24. Yokosuka T., Sakata-Sogawa K., Kobayashi W., Hiroshima M., Hashimoto-Tane A., Tokunaga M., et al. Newly generated T cell receptor microclusters initiate and sustain T cell activation by recruitment of Zap70 and SLP-76. Nature Immunology 2005, 6:1253-1262.
25. Zhang J., Leiderman K., Pfeiffer J.R., Wilson B.S., Oliver J.M., Steinberg S.L. Characterizing the topography of membrane receptors and signaling molecules from spatial patterns obtained using nanometer-scale electron-dense probes and electron microscopy. Micron 2006, 37:14-34.