Biochemical and structural characterization of recombinant short-chain NAD(H)-dependent dehydrogenase/reductase from Sulfolobus acidocaldarius highly enantioselective on diaryl diketone benzil

Applied Microbiology and Biotechnology

Volumn 97, Issue 9, 2013, Pages 3949-3964

  Pennacchio, Angela ^a   Sannino, Vincenzo ^a   Sorrentino, Giosuè ^b   Rossi, Mosè ^a   Raia, Carlo A ^a   Esposito, Luciana ^b  

^a INSTITUTE OF PROTEIN BIOCHEMISTRY   (Italy)

^b CNR   (Italy)

Author keywords

Archaea; Benzil; Bioreduction; Crystal structure; Short chain dehydrogenases reductases; Sulfolobus acidocaldarius

Indexed keywords

AROMATIC COMPOUNDS; COENZYMES; DEHYDROGENATION; ENANTIOSELECTIVITY; ESCHERICHIA COLI; GENE ENCODING; KETONES;

ARCHAEA; BENZIL; BIO REDUCTIONS; SHORT-CHAIN DEHYDROGENASES/REDUCTASES; SULFOLOBUS ACIDOCALDARIUS;

CRYSTAL STRUCTURE;

ACETIC ACID ETHYL ESTER; ACETONITRILE; ALCOHOL DERIVATIVE; ALDEHYDE DERIVATIVE; BACTERIAL ENZYME; DIMETHYL SULFOXIDE; DIOXANE; KETONE DERIVATIVE; RECOMBINANT ENZYME; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEHYDROGENASE; SAADH2 ENZYME; UNCLASSIFIED DRUG; ARCHAEAL PROTEIN; BENZIL; BENZOIN; DRUG DERIVATIVE; FATTY ACID SYNTHASE; OXIDOREDUCTASE; PHENYLGLYOXAL; SHORT CHAIN TRANS 2 ENOYL COA REDUCTASE; SHORT CHAIN TRANS-2-ENOYL-COA REDUCTASE;

BACTERIUM; BIOCHEMICAL COMPOSITION; CRYSTAL STRUCTURE; ENZYME ACTIVITY; RECOMBINATION; SOLVENT;

ARTICLE; BACTERIAL GENOME; BINDING SITE; BIOTRANSFORMATION; COENZYME; CRYSTAL STRUCTURE; ELECTROSPRAY MASS SPECTROMETRY; ENANTIOMER; ENANTIOSELECTIVITY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SPECIFICITY; HETEROLOGOUS EXPRESSION; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; PH; PRECIPITATION; PROTEIN QUATERNARY STRUCTURE; SOLUBILITY; SUCROSE DENSITY GRADIENT CENTRIFUGATION; SULFOLOBUS ACIDOCALDARIUS; TEMPERATURE; THERMOSTABILITY; AMINO ACID SEQUENCE; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; GENETICS; METABOLISM; MOLECULAR GENETICS; SEQUENCE HOMOLOGY; STEREOISOMERISM;

CRENARCHAEOTA; ESCHERICHIA COLI; SULFOLOBUS ACIDOCALDARIUS;

AMINO ACID SEQUENCE; ARCHAEAL PROTEINS; BENZOIN; ENZYME STABILITY; FATTY ACID SYNTHETASE COMPLEX; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; NADH, NADPH OXIDOREDUCTASES; PHENYLGLYOXAL; SEQUENCE HOMOLOGY, AMINO ACID; STEREOISOMERISM; SUBSTRATE SPECIFICITY; SULFOLOBUS ACIDOCALDARIUS;

EID: 84885903482     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4273-z     Document Type: Article

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