Evidence for co-operativity in coenzyme binding to tetrameric Sulfolobus solfataricus alcohol dehydrogenase and its structural basis: Fluorescence, kinetic and structural studies of the wild-type enzyme and non-co-operative N249Y mutant

Biochemical Journal

Volumn 388, Issue 2, 2005, Pages 657-667

  Giordano, Antonietta ^a   Febbraio, Ferdinande ^a   Russo, Consiglia ^a   Rossi, Mosè ^a   Raia, Carlo A ^a  

^a INSTITUTE OF PROTEIN BIOCHEMISTRY   (Italy)

Author keywords

Archaeal alcohol dehydrogenase; Co operativity; Coenzyme binding; Fluorescence quenching; Hill coefficient; Intersubunit communication

Indexed keywords

FLUORESCENCE; IONIC STRENGTH; MUTAGENESIS; ORGANIC COMPOUNDS; PH EFFECTS; QUENCHING; REACTION KINETICS;

COENZYME BINDING; MUTANTS; THERMOPHILIC ENZYMES; TRYPTOPHAN;

COENZYMES;

ALCOHOL DEHYDROGENASE; NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; TRYPTOPHAN;

ARTICLE; COENZYME; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME BINDING; ENZYME STRUCTURE; FLUORESCENCE ANALYSIS; HILL REACTION; IONIC STRENGTH; MICHAELIS MENTEN KINETICS; NONHUMAN; OXIDATION; PH; PRIORITY JOURNAL; SULFOLOBUS SOLFATARICUS; TEMPERATURE; THERMOSTABILITY; WILD TYPE;

ALCOHOL DEHYDROGENASE; ALLOSTERIC REGULATION; AMINO ACID SEQUENCE; COENZYMES; ENZYME STABILITY; HEAT; HYDROGEN-ION CONCENTRATION; KINETICS; POINT MUTATION; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN SUBUNITS; SODIUM CHLORIDE; SPECTROMETRY, FLUORESCENCE; SULFOLOBUS SOLFATARICUS;

ARCHAEA; SULFOLOBUS SOLFATARICUS;

EID: 20544459615     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20041539     Document Type: Article

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