Purification and characterization of the alcohol dehydrogenase from a novel strain of Bacillus stearothermophilus growing at 70°C

International Journal of Biochemistry and Cell Biology

Volumn 28, Issue 2, 1996, Pages 239-246

  Guagliardi, Annamaria ^a   Martino, Matilde ^a   Iaccarino, Ingram ^a   De Rosa, Mario ^a   Rossi, Mosè ^a,b   Bartolucci, Simonetta ^a  

^a UNIVERSITY OF NAPLES FEDERICO II   (Italy)

^b CNR   (Italy)

Author keywords

Alcohol dehydrogenase; Bacillus stearothermophilis; Thermal resistance

Indexed keywords

ALCOHOL DEHYDROGENASE;

ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CONTROLLED STUDY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SPECIFICITY; GEOBACILLUS STEAROTHERMOPHILUS; HEAT TOLERANCE; NONHUMAN;

EID: 0030052553     PISSN: 13572725     EISSN: None     Source Type: Journal    
DOI: 10.1016/1357-2725(95)00138-7     Document Type: Article

References (29)

1. Adolph H. W., Maurer P., Schneider-Bernlohr H., Sartorius C. and Zeppezauer M. (1991) Substrate specificity and stereoselectivity of horse liver alcohol dehydrogenase. Eur. J. Biochem. 201, 615-625.
2. Ammendola S., Raia C. A., Caruso C., Camardella L., D'Auria S., De Rosa M. and Rossi M. (1992) Thermostable NAD-dependent alcohol dehydrogenase from Sulfolobus solfataricus: gene and protein sequence determination and relationship to other alcohol dehydrogenases. Biochemistry 31, 12514-12523.
3. Bradford M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
4. Bränden C.-I., Jörnvall H., Eklund H. and Furugren B. (1975) In The Enzymes (Edited by Boyer P. D.), Vol. 11A, pp. 103-190. Academic Press, New York.
5. Bryant F. O., Weigel J. and Ljungdahl L. G. (1988) Purification and properties of primary and secondary alcohol dehydrogenases from Thermoanaerobacter ethanolicus. Appl. Environ. Microbiol. 54, 460-465.
6. Cannio R., Rossi M. and Bartolucci S. (1994) A few amino acid substitutions are responsible for the higher thermostability of a novel NAD-dependent bacillar alcohol dehydrogenase. Eur. J. Biochem. 222, 345-352.
7. Cederlund E., Peralba J. M., Pares X. and Jörnvall H. (1991) Amphibian alcohol dehydrogenase, the major frog liver enzyme. Relationships to other forms and assessment of an early gene duplication separating vertebrate class I and class II alcohol dehydrogenases. Biochemistry 30, 2811-2816.
8. Coolbear T., Daniel R. M. and Morgan H. W. (1992) The enzymes from extreme thermophiles: bacterial sources, thermostabilities and industrial relevance. Adv. Biochem. Eng. Biotechnol. 45, 57-98.
9. Cowan D. A. (1992) In The Archaebacteria: Biochemistry and Biotechnology (Edited by Danson M. J., Hough D. W. and Lunt G. G.), Biochemical Society Symposium No. 58, pp. 149-169. Portland Press, London.
10. Davis B. J. (1964) Disc electrophoresis II method and application to human pheromone proteins. Ann. N.Y. Acad. Sci. 121, 404-427.
11. Donk P. J. (1920) A highly resistant thermophilic organism. J. Bacteriol. 5, 373.
12. Guagliardi A., Manco G., Rossi M. and Bartolucci S. (1989) Stability and activity of a thermostable malic enzyme in denaturants and water miscible organic solvents. Eur. J. Biochem. 183, 25-30.
13. Guagliardi A., Raia C. A., Relia R., Bückmann A. F., D'Auria S., Rossi M. and Bartolucci S. (1991) Coenzy-matic properties of macromolecular derivatives of NAD and NADP with two thermostable dehydrogenases from the archaebacterium Sulfolobus solfataricus. Biotechnol. Appl. Biochem. 13, 25-35.
14. Hartley B. S. (1988) Int. Patent Appl. PCT/GB/8800411.
15. Hummel W. and Kula M. R. (1989) Dehydrogenases for the synthesis of chiral compounds. Eur. J. Biochem. 184, 1-13.
16. Keinan E., Hafeli E. K., Seth K. K. and Lamed R. (1986) Thermostable enzymes in organic synthesis. 2. Asymmetric production of ketones with alcohol dehydrogenase from Thermoanaerobium brockii. J. Am. Chem. Soc. 108, 162-169.
17. Keinan E., Seth K. K. and Lamed R. (1987) Synthetic applications of alcohol dehydrogenases from Thermoanaerobium brockii. Ann. N. Y. Acad. Sci. 501, 130-150.
18. Laemmli U. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
19. Lamed R. J. and Zeikus J. G. (1981) Novel NADP-linked alcohol aldehyde/ketone oxidoreductase in thermophilic ethanologenic bacteria. Biochem. J. 105, 183-190.
20. Pham V. T., Phillips R. S. and Ljungdahl L. G. (1989) Temperature-dependent enantiospecificity of secondary alcohol dehydrogenase from Thermoanaerobacter ethanolicus. J. Am. Chem. Soc. 11, 1935-1936.
21. Pham V. T. and Phillips R. S. (1990) Effects of substrate structure and temperature on the stereospecificity of secondary alcohol dehydrogenase from Thermoanaerobacter ethanolicus. J. Am. Chem. Soc. 112, 3629-3632.
22. Rella R., Raia C. A., Trincone A., Gambacorta A., De Rosa M. and Rossi M. (1987a) In Biocatalysis in Organic Media (Edited by Laane C., Tramper J. and Lilly M. D.), Vol. 29, pp. 273-278. Elsevier, Amsterdam.
23. Rella R., Raia C. A., Pensa M., Pisani F. M., Gambacorta A., De Rosa M. and Rossi M. (1987b) A novel archaebacterial NAD-depenent alcohol dehydrogenase. Eur. J. Biochem. 167, 475-479.
24. Righetti P. E. and Drysdale J. W. (1976) In Laboratory Techniques in Biochemistry and Molecular Biology (Edited by Work T. S. and Work E.), Vol. 5, part 2. Elsevier, Amsterdam.
25. Runswick M. J. and Harris J. I. (1978) Purification of alchol dehydrogenase from Bacillus stearothermophilus by affinity chromatography. FEBS Lett. 92, 365-367.
26. Simpson H. D. and Cowan D. A. (1992) Secondary alcohol dehydrogenases from extremely thermophilic bacteria. Ann. N.Y. Acad. Sci. 672, 145-151.
27. Sund H. and Theorell H. (1963) In The Enzymes (Edited by Boyer P. D., Lardy H. and Myrback K.), Vol. 7, pp. 25-83. Academic Press, New York.
28. Trincone A., Lama L., Lanzotti V., Nicolaus B., De Rosa M., Rossi M. and Gambacorta A. (1990) Asymmetric reduction of ketones with resting cells of Sulfolobus solfataricus. Biotechnol. Bioengng. 35, 559-564.
29. Wrba A., Schweiger A., Schultes V. and Jaenicke R. (1990) Extremely thermostable D-glyceraldehyde-3-phosphate dehydrogenase from the eubacterium Thermotoga maritima. Biochemistry 29, 7584-7592.