N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes

Redox Report

Volumn 18, Issue 6, 2013, Pages 210-218

  Calzadilla, P ^a   Gomez Serrano M ^b   Garcia Santos E ^b   Schiappacasse, A ^a   Abalde, Y ^a   Calvo, J C ^c   Peral, B ^b   Guerra, L N ^a  

^a UNIVERSIDAD DE BUENOS AIRES   (Argentina)

^b INSTITUTO DE INVESTIGACIONES BIOMÉDICAS ALBERTO SOLS   (Spain)

^c NONE   (Argentina)

Author keywords

Adipose differentiation; N acetylcysteine; Obesity proteins

Indexed keywords

ACETYLCYSTEINE; AMINE OXIDASE (FLAVIN CONTAINING) ISOENZYME A; AMINOACYLASE; FATTY ACID BINDING PROTEIN 4; HEAT SHOCK PROTEIN 70; TRANSKETOLASE; TRIACYLGLYCEROL;

ADIPOGENESIS; AMINO ACID METABOLISM; ANIMAL CELL; ARTICLE; CARBOHYDRATE METABOLISM; CELL DIFFERENTIATION; CELL MATURATION; CELL PROLIFERATION; CELL STRAIN 3T3; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG EFFECT; DRUG MECHANISM; LIPID STORAGE; LIPOGENESIS; MOUSE; NONHUMAN; OBESITY; OXIDATIVE STRESS; PRIORITY JOURNAL; PROADIPOCYTE; PROTEIN EXPRESSION;

3T3-L1 CELLS; ACETYLCYSTEINE; ADIPOCYTES; ADIPOGENESIS; AMIDOHYDROLASES; ANIMALS; CELL DIFFERENTIATION; FATTY ACID-BINDING PROTEINS; HSP72 HEAT-SHOCK PROTEINS; MICE; MONOAMINE OXIDASE; OXIDATIVE STRESS; TRANSKETOLASE; TRIGLYCERIDES;

EID: 84885087762     PISSN: 13510002     EISSN: 17432928     Source Type: Journal    
DOI: 10.1179/1351000213Y.0000000066     Document Type: Article

References (44)

1. Spiegeman BM, Fliers JS. Obesity and regulation of energy balance. Cell 2001;104:531-43.
2. Calvo JC, Chernick S, Rodbard D. Mouse mammary epithelium produces a soluble heat-sensitive macromolecule that inhibits the differentiation of 3T3-L1 preadipocytes. Proc Soc Exp Biol Med 1992;201:174-9.
3. Green H, Kehinde O. Sublines of mouse 3T3 cells that accumulate lipid. Cell 1974;4:113-6.
4. Green H, Kehinde O. An established preadipose cell line and its differentiation in culture: factors affecting the adipose conversion. Cell 1975;5:19-27.
5. Mac Dougald OA, Lane MD. Transcriptional regulation of gene expression during adipocyte differentiation. Annu Rev Biochem 1995;64:345-58.
6. Darlington GJ, Ross SE, Mac Douglas OA. The role of C/EBP genes in adipocyte differentiation. J Biol Chem 1996;273: 30057-60.
7. Hemati N, Ross SE, Erickson RL, Groblewski GE, Mac Douglas OA. Signaling pathways through which insulin regulates CCAAT/Enhancer Binding Protein alpha phosphorylation and gene expression in 3T3-L1 adipocytes. J Biol Chem 1997; 272:25913-19.
8. Tontonoz P, Hu E, Spiegelman BM. Stimulation of adipogenesis in fibroblast by PPARg, a lipid-activated transcription factor. Cell 1994;79:1147-65.
9. Keany JF, Larson MG, Vasan RS. Obesity and systemic oxidative stress: clinical correlates of oxidative stress in Framingham Study. Arterioscler Thromb Vasc Biol 2003;23: 434-9.
10. McClung J, Roneker C, Mu W, Lisk D, Langlais P, Liu F, Lei XG. Development of insulin resistant and obesity in mice overexpressing cellular glutathione peroxidase. Proc Natl Acad Sci USA 2004;101:8852-7.
11. Sapochnick D, Julianelli V, Garate H, Calvo JC, Guerra LN. Oxidative metabolism during preadipocytes differentiation. Free Radicals in Montevideo Book of Abstracts. Montevideo: SFRBM Press; 2007. p. 60.
12. Kim JR, Ryu H, Chung HJ, Lee JH, Kim SW, Kwun WH, et al. Association of anti-obesity activity of N-acetylcysteine with mettallothionein-II down-regulation. Exp Mol Med 2006;30: 162-72.
13. Araki S, Dobashi K, Kugo K, Yamamoto Y, Asayama K, Shirahata A. N-acetylcysteine attenuates TNF-alpha induced changes in secretion of interleukin-6, plasminogen activator inhibitor-1 and adiponectin from 3T3-L1 adipocytes. Life Sci 2006;79:2405-12.
14. Wang T, Si Y, Shirihai OS, Si H, Schultz V, Corkey RF, et al. Respiration in adipocytes is inhibited by reactive oxygen species. Obesity 2010;18:1493-502.
15. Guo W, Li Y, Liang W, Wong S, Apovian C, Kirkland B, et al. Beta-mercaptoethanol suppresses inflammation and induces adipogenic differentiation in 3T3-F442 murine preadipocytes. PLoS One 2012;7:e40958.
16. Miler EA, Rios MC, Dominguez G, Guerra LN. Thyroid hormone effect in human hepatocytes. Red Rep 2008;13:185-91.
17. Miler E, Burdman JA, Ríos de Molina MC, Bechara Y, Guerra LN. Thyroid hormone and oxidative metabolism. Free Radical Research Internacional. Bologna: Monduzzi Editors; 2004. p. 329-33.
18. Guerra LN, Rios de Molina MC, Miler EA, Moiguer S, Karner M, Burdman JA. Antioxidants and methimazole in the treatments of Graves' disease: effect on urinary malondialdehyde levels. Clin Chim Acta 2005;352:115-20.
19. Guerra LN, Moiguer S, Karner M, Rios de Molina MC, Sreider C, Burdman JA. Antioxidants in the treatment of Graves Disease. IUBMB Life 2001;51:105-9.
20. Calzadilla P, Sapochnick D, Cosentino S, Diz V, Dicelio L, Calvo JC, et al. N-acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes. Int J Mol Sci 2011;12:6936-51.
21. Perez-Perez R, Garcia-Santos E, Ortega-Delgado FJ, Lopez JA, Camafeita E, Ricart W, et al. Attenuated metabolism is a hallmark of obesity as revealed by comparative proteomic analysis of human omental adipose tissue. J Proteomics 2012;75: 783-95.
22. Julianelli VL, Guerra LN, Calvo JC. Cell-cell communication between mouse mammary epithelial cells and 3T3 T-L1 preadipocytes: Effect on triglyceride accumulations and cell proliferation. Biocell 2007;31:237-45.
23. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248-54.
24. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.
25. Analytical Software, Statistix 8, Tallahassee, FL, USA: McGraw-Hill/Irwin, 2003.
26. Natarajan M, Mohan S, Martinez BR, Meltz ML, Herman TS. Antioxidant compounds interfere with the 3. Cancer Detect Prev 2000;24:405-14.
27. Gummersbach C, Hemmrich K, Klaus-Dietrich K, Suschek CV, Fehsel K, Pallua N. New aspect of adipogenesis: radicals and oxidative stress. Differentiation 2009;77:115-20.
28. Lee H, Jeong Y, Choi H, Ko E, Kim J. Reactive oxygen species facilitates adipocyte differentiation by accelerating mitotic clonal expansion. J Biol Chem 2009;284:10601-9.
29. Kanda Y, Hinata T, Kang SW, Watanabe Y. Reactive oxygen species mediate adipocyte differentiation in mesenchymall stem cells. Life Sci 2011;89:250-8.
30. Lin Y, Berg AH, Iyengar P, Lam T, Giacca A, Combs T, et al. The hyperglycemia-induced inflammatory response in adipocytes. The role of reactive oxygen species. J Biol Chem 2005; 280:4617-26.
31. Shao HY, Hsu HY, Wu KS, Hee SW, Chuang LM, Yeh JI. Prolonged induction activates C/EBP a independent adipogenesis in NID/3T3-L1 cells. PLoS One 2013;8:e51459.
32. Chung J, Nguyen AK, Henstridge DC, Holmes AG, Chan MH, Mesa JL. HSP72 protects against obesity-induced insulin resistance. Proc Natl Acad Sci. U S A 2008;105:1739-44.
33. Uchida Y, Takeshita K, Yamamoto K, Kikuchi R, Nakayama T, Nomura M, et al. Stress augments insulin resistance and prothrombotic state: role of visceral adipose-derived monocyte chemoattractant protein-1. Diabetes 2012;61:1552-61.
34. Imagawa M, Tsuchiya T, Nishihara T. Identification of inducible genes at the early stage of adipocyte differentiation of 3T3-L1 cells. Biochem Biophys Res Commun 1999;254: 299-305.
35. Gallo L, Lagadari M, Piwien-Pilipuk G, Galigniana MD. The 90-kDa heat-shock protein (Hsp90)-binding immunophilin FKBP51 is a mitochondrial protein that translocates to the nucleus to protect cells against oxidative stress. J Biol Chem 2011;286:30152-160.
36. Fuemmeler BF, Agurs-Collins T, McClernon FJ, Kollins SH. Genes implicated in serotonergic and dopaminergic functioning predict BMI categories. Obesity 2008;16:348-55.
37. Pizzinat N, Marti L, Remaury A, Leger F, Langin D, Lafontan M. High expression of monoamine oxidase in human white tissue: evidence for their involvement in noradrenaline clearance. Biochemical Pharmacol 1999;58:1735-42.
38. Mangge H, Summers K, Almer G, Prass R, Weghuber D, Schndel W, et al. Antioxidant food supplements and obesityrelated inflammation. Curr Med Chem 2013;20:2330-7.
39. Ciardi C, Jenny M, Tschoner A, Ueberall F, Patsch J, Pedrini M, et al. Food additives such as sodium sulphite, sodium benzoate and curcumin inhibit leptin release in lipopolysaccharidetreated murine adipocytes in vitro. Br J Nutr 2012;107:826-33.
40. Xie XY, Kong PR, Wu JF, Li Y, Li YX. Curcumin attenuates lipolysis stimulated by tumor necrosis factor-α or isoproterenol in 3T3-L1 adipocytes. Phytomedicine 2012;20:3-8.
41. Zingg JM, Hasan ST, Meydani M. Molecular mechanisms of hypolipidemic effects of curcumin. Biofactors 2013;39:101-21.
42. Liu C, Kuei C, Zhu J, Yu J, Zhang L, Shih A, et al. 3,5-Dihydroxybenzoic acid a specific agonist for hydroxycarboxylic acid 1, inhibits lipolysis in adipocytes. J Phar Exp Ther 2012;341:794-801.
43. Uto-Kondo H, Ohmori R, Kiyose C, Kishimoto Y, Saito H, Igarashi O, et al. Tocotrienol suppresses adipocyte differentiation and AKT phosphorylation in 3T3-L1 preadipocytes. J Nutr 2009;139:51-7.
44. Hsu CL, Yen GC. Effect of flavonoids and phenolic acid on the inhibition of adipogenesis in 3T3-L1 adipocytes. J Agric Food Chem 2007;17:8404-10.