Antioxidant compounds interfere with the 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide cytotoxicity assay

Cancer Detection and Prevention

Volumn 24, Issue 5, 2000, Pages 405-414

  Natarajan, M ^a   Mohan, S ^a   Martinez, B R ^a   Meltz, M L ^a   Herman, T S ^a  

^a UNIVERSITY OF TEXAS HEALTH SCIENCE CENTER AT SAN ANTONIO   (United States)

Author keywords

Antioxidants; Cytotoxicity; Drugs; In vitro; MTT assay

Indexed keywords

2 OXO 4 THIAZOLIDINECARBOXYLIC ACID; 2-OXOTHIAZOLIDINE-4-CARBOXYLIC ACID; ACETYLCYSTEINE; ALPHA TOCOPHEROL; ANTIOXIDANT; ASCORBIC ACID; COLORING AGENT; DITHIOTHREITOL; MELATONIN; MERCAPTOETHANOL; PYROGLUTAMIC ACID; PYRROLIDINE DERIVATIVE; PYRROLIDINE DITHIOCARBAMIC ACID; TETRAZOLIUM; THIAZOLE DERIVATIVE; THIAZOLIDINE DERIVATIVE; THIAZOLYL BLUE; THIOCARBAMIC ACID DERIVATIVE; THIOL DERIVATIVE;

ANALYTICAL EQUIPMENT; ARTICLE; CHEMISTRY; COLORIMETRY; CULTURE MEDIUM; DRUG EFFECT; KINETICS; METHODOLOGY; OXIDATION REDUCTION REACTION;

ACETYLCYSTEINE; ANTIOXIDANTS; ASCORBIC ACID; COLORIMETRY; COLORING AGENTS; CULTURE MEDIA; DITHIOTHREITOL; KINETICS; MELATONIN; MERCAPTOETHANOL; OXIDATION-REDUCTION; PYRROLIDINES; PYRROLIDONECARBOXYLIC ACID; REAGENT KITS, DIAGNOSTIC; SULFHYDRYL COMPOUNDS; TETRAZOLIUM SALTS; THIAZOLES; THIAZOLIDINES; THIOCARBAMATES; VITAMIN E;

EID: 0033670268     PISSN: 0361090X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (47)

1. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays
2. A new approach for measurement of cytotoxicity using colorimetric assay
3. Studies on succinate-tetrazolium reductase system. Points of coupling of four different tetrazolium salts
4. Characterization of oxidative injury to an intestinal cell line (HT-29) by hydrogen peroxide
5. Tetrazolium-based assays for cellular viability: A critical examination of selected parameters affecting formazan production
6. Feasibility of drug screening with panels of human tumor cell lines using a micro-culture tetrazolium assay
7. A rapid and simple colorimetric test for the study of anti-HIV agents
8. Measurement of cell numbers by means of the endogenous enzyme hexosaminidase. Application to detection of lymphokines and cell surface antigens
9. Rapid colorimetric assay for cell growth and survival. Modification to this tetrazolium dye procedure giving improved sensitivity and reliability
10. Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill
11. An improved colorimetric assay for interleukin 2
12. Characterization of the cellular reduction of 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT): Subcellular localization, substrate dependence, and involvement of mitochondrial electron transport in MTT reduction
13. Tetrazolium salts and formazans
14. Reduction of tetrazolium salt and superoxide generation in human tumor cells (HeLa)
15. Mechanism of cellular 3-(4, 5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction
16. A simplified in vitro model of oxidant injury using vascular endothelial cells
17. Evaluation of a tetrazolium-based semiautomated colorimetric assay: Assessment of chemosensitivity testing
18. Oxidized low-density lipoprotein caused CNS neuron cell death
19. Studies of the neurotoxicity of 6,7-dihydroxy-1-methyl-1, 2,3,4-tetrahydroisoquinoline (salsolinol) in SH-SY5Y cells
20. Role of oxidative stress in the manganese and 1-methyl-4-(2'-ethylphenyl)-1,2,3,6-tetrahydropyridine-induced apoptosis in PC12 cells
21. Mitochondrial function impairment induced by amyloid beta-peptide on PC12 cells
22. Pycnogenol protects vascular endothelial cells from t-butyl hydroperoxide induced oxidant injury
23. Intracellular cysteine and glutathione delivery system
24. Evaluation of the antioxidant activity of melatonin in vitro
25. Nutritional antioxidants and the modulation of inflammation: Theory and practice
26. Dithiocarb sodium (diethyldithiocarbamate) therapy in patients with symptomatic HIV infection and AIDS
27. Inhibition of activation-induced death in T cell hybridomas by thiol antioxidant: Oxidative stress as a mediator of apoptosis
28. Effects of beta mercaptoethanol on the proliferation and differentiation of human osteoprogenitor cells
29. Changes in intracellular glutathione levels in stimulated and unstimulated lymphocytes in the presence of 2-mercaptoethanol or cysteine
30. Promotion of development of bovine embryos produced in vitro by addition of cysteine and beta-mercaptoethanol to a chemically defined culture system
31. In vitro antioxidant treatment recovers proliferative responses of anergic CD4+ lymphocytes from human immunodeficiency virus-infected individuals
32. Suppression of human immunodeficiency virus expression in chronically infected monocytic cells by glutathione, glutathione ester, and N-acetylcysteine
33. Clinical applications of N-acetylcysteine
34. Chemopreventive properties and mechanism of N-acetylcysteine. The experimental background
35. Antioxidants reduce cyclooxygenase-2 expression, prostaglandin production, and proliferation in colorectal cancer cells
36. Activity and distribution of the cysteine prodrug activating enzyme, 5-oxo-L-prolinase, in human normal and tumor tissues
37. Ascorbic acid in the prevention and treatment of cancer
38. Growth-inhibitory effects of vitamin E succinate on retrovirus-transformed tumor cells in vitro
39. Induction of NF-kB after low dose ionizing radiation involves ROI signaling pathway
40. Neuronal hormone melatonin inhibits TNF-a, PMA and ionizing radiation induced NF-kB
41. Thiols and selenium: Protective effect on human skin fibroblasts exposed to UVA radiation
42. Protection by vitamin C of loss of vitamin E in cultured rat hepatocytes
43. Differential regulation of low shear stress induced NF-kB activation, VCAM-1 expression and monocyte adhesion in human aortic endothelial cells by selected antioxidants
44. Manganese and 1-methyl-4-(2'-ethylphenyl)-1, 2, 3, 6-tetrahydropyridine induce apoptosis in PC12 cells
45. Chromium-and nickel-induced cytotoxicity in normal and transformed human keratinocytes: An investigation of pharmacological approaches to the prevention of Cr (VI)-induced cytotoxicity
46. Effect of ascorbic acid on DNA damage, cytotoxicity, glutathione reductase, and formation of paramagnetic chromium in Chinese hamster V-79 cells treated with sodium chromate (VI)
47. Reduction of MTT by glutathione S-transferase