Prolonged Induction Activates Cebpα Independent Adipogenesis in NIH/3T3 Cells

PLoS ONE

Volumn 8, Issue 1, 2013, Pages

  Shao, Hsiao Yun ^a,d   Hsu, Hsue Yin ^a   Wu, Kuan Sju ^a   Hee, Siow Wey ^b   Chuang, Lee Ming ^b   Yeh, Jih I ^a,c  

^a TZU CHI UNIVERSITY   (Taiwan)

^b NATIONAL TAIWAN UNIVERSITY   (Taiwan)

^c BUDDHIST TZU CHI GENERAL HOSPITAL   (Taiwan)

^d NATIONAL HEALTH RESEARCH INSTITUTES   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

ANGIOTENSINOGEN; CCAAT ENHANCER BINDING PROTEIN ALPHA; DEOXYGLUCOSE; FAT DROPLET; FATTY ACID BINDING PROTEIN 4; GLUCOSE TRANSPORTER 4; INSULIN; MESSENGER RNA; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA; PHOSPHATIDYLINOSITOL 3 KINASE; PROTEIN KINASE B; ROSIGLITAZONE; SHORT HAIRPIN RNA; TRIACYLGLYCEROL;

ADIPOCYTE; ADIPOGENESIS; ADIPOSE TISSUE; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CARBOHYDRATE TRANSPORT; CELL DIFFERENTIATION; CELL STRAIN 3T3; CONTROLLED STUDY; CULTURE MEDIUM; DIABETES MELLITUS; FABP4 GENE; GENE EXPRESSION; GENE SILENCING; GENETIC ENGINEERING; INCUBATION TIME; LIPID STORAGE; MOUSE; NONHUMAN; PPARG GENE; PROTEIN INDUCTION; SIGNAL TRANSDUCTION; SLC2A4 GENE;

3T3-L1 CELLS; ADIPOCYTES; ADIPOGENESIS; ANIMALS; BIOLOGICAL TRANSPORT; BLOTTING, WESTERN; CATTLE; CCAAT-ENHANCER-BINDING PROTEIN-ALPHA; CELL DIFFERENTIATION; CULTURE MEDIA; DEOXYGLUCOSE; FATTY ACID-BINDING PROTEINS; GENE EXPRESSION; HYPOGLYCEMIC AGENTS; INSULIN; MICE; MICE, INBRED C57BL; NIH 3T3 CELLS; PPAR GAMMA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA INTERFERENCE; SERUM; THIAZOLIDINEDIONES; TIME FACTORS; TRIGLYCERIDES;

BOVINAE;

EID: 84872227992     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0051459     Document Type: Article

References (24)

1. Rosen ED, MacDougald OA, (2006) Adipocyte differentiation from the inside out. Nat Rev Mol Cell Biol 7: 885-896.
2. Green H, Kehinde O, (1975) An established preadipose cell line and its differentiation in culture. II. Factors affecting the adipose conversion. Cell 5: 19-27.
3. Green H, Meuth M, (1974) An established pre-adipose cell line and its differentiation in culture. Cell 3: 127-133.
4. Rubin CS, Hirsch A, Fung C, Rosen OM, (1978) Development of hormone receptors and hormonal responsiveness in vitro. Insulin receptors and insulin sensitivity in the preadipocyte and adipocyte forms of 3T3-L1 cells. J Biol Chem 253: 7570-7578.
5. Jainchill JL, Aaronson SA, Todaro GJ, (1969) Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells. J Virol 4: 549-553.
6. El-Jack AK, Hamm JK, Pilch PF, Farmer SR, (1999) Reconstitution of insulin-sensitive glucose transport in fibroblasts requires expression of both PPARgamma and C/EBPalpha. J Biol Chem 274: 7946-7951.
7. Wu Z, Rosen ED, Brun R, Hauser S, Adelmant G, et al. (1999) Cross-regulation of C/EBP alpha and PPAR gamma controls the transcriptional pathway of adipogenesis and insulin sensitivity. Mol Cell 3: 151-158.
8. Rosen ED, Hsu CH, Wang X, Sakai S, Freeman MW, et al. (2002) C/EBPalpha induces adipogenesis through PPARgamma: a unified pathway. Genes Dev 16: 22-26.
9. Lehmann JM, Moore LB, Smith-Oliver TA, Wilkison WO, Willson TM, et al. (1995) An antidiabetic thiazolidinedione is a high affinity ligand for peroxisome proliferator-activated receptor gamma (PPAR gamma). J Biol Chem 270: 12953-12956.
10. Lambe KG, Tugwood JD, (1996) A human peroxisome-proliferator-activated receptor-gamma is activated by inducers of adipogenesis, including thiazolidinedione drugs. Eur J Biochem 239: 1-7.
11. Guo W, Zhang KM, Tu K, Li YX, Zhu L, et al. (2009) Adipogenesis licensing and execution are disparately linked to cell proliferation. Cell Res 19: 216-223.
12. Wang M, Bronte V, Chen PW, Gritz L, Panicali D, et al. (1995) Active immunotherapy of cancer with a nonreplicating recombinant fowlpox virus encoding a model tumor-associated antigen. J Immunol 154: 4685-4692.
13. Kaneko T, LePage GA, (1978) Growth characteristics and drug responses of a murine lung carcinoma in vitro and in vivo. Cancer Res 38: 2084-2090.
14. Yeh WC, Cao Z, Classon M, McKnight SL, (1995) Cascade regulation of terminal adipocyte differentiation by three members of the C/EBP family of leucine zipper proteins. Genes Dev 9: 168-181.
15. Payne VA, Au WS, Gray SL, Nora ED, Rahman SM, et al. (2007) Sequential regulation of diacylglycerol acyltransferase 2 expression by CAAT/enhancer-binding protein beta (C/EBPbeta) and C/EBPalpha during adipogenesis. J Biol Chem 282: 21005-21014.
16. Yki-Jarvinen H, (2004) Thiazolidinediones. N Engl J Med 351: 1106-1118.
17. Raji A, Seely EW, Bekins SA, Williams GH, Simonson DC, (2003) Rosiglitazone improves insulin sensitivity and lowers blood pressure in hypertensive patients. Diabetes Care 26: 172-178.
18. Sarafidis PA, Lasaridis AN, Nilsson PM, Pagkalos EM, Hitoglou-Makedou AD, et al. (2004) Ambulatory blood pressure reduction after rosiglitazone treatment in patients with type 2 diabetes and hypertension correlates with insulin sensitivity increase. J Hypertens 22: 1769-1777.
19. Wang ND, Finegold MJ, Bradley A, Ou CN, Abdelsayed SV, et al. (1995) Impaired energy homeostasis in C/EBP alpha knockout mice. Science 269: 1108-1112.
20. Chen SS, Chen JF, Johnson PF, Muppala V, Lee YH, (2000) C/EBPbeta, when expressed from the C/ebpalpha gene locus, can functionally replace C/EBPalpha in liver but not in adipose tissue. Mol Cell Biol 20: 7292-7299.
21. Darlington GJ, Ross SE, MacDougald OA, (1998) The role of C/EBP genes in adipocyte differentiation. J Biol Chem 273: 30057-30060.
22. Bakris G, Viberti G, Weston WM, Heise M, Porter LE, et al. (2003) Rosiglitazone reduces urinary albumin excretion in type II diabetes. J Hum Hypertens 17: 7-12.
23. Stephens JM, Lee J, Pilch PF, (1997) Tumor necrosis factor-alpha-induced insulin resistance in 3T3-L1 adipocytes is accompanied by a loss of insulin receptor substrate-1 and GLUT4 expression without a loss of insulin receptor-mediated signal transduction. J Biol Chem 272: 971-976.
24. Schneider CA, Rasband WS, Eliceiri KW, (2012) NIH Image to ImageJ: 25 years of image analysis. Nat Methods 9: 671-675.