Expression, surface immobilization, and characterization of functional recombinant cannabinoid receptor CB2

Biochimica et Biophysica Acta - Proteins and Proteomics

Volumn 1834, Issue 10, 2013, Pages 2045-2056

  Locatelli Hoops, Silvia C ^a   Gorshkova, Inna ^b   Gawrisch, Klaus ^a   Yeliseev, Alexei A ^a  

^a NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM   (United States)

^b NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING   (United States)

Author keywords

Cannabinoid receptor; CB2; Functional immobilization; Rho tag; Surface plasmon resonance (SPR)

Indexed keywords

CANNABINOID RECEPTOR; RECOMBINANT RECEPTOR; RHODOPSIN;

ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; HUMAN; LIGAND BINDING; MICELLE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN IMMOBILIZATION; SURFACE PLASMON RESONANCE; SURFACE PROPERTY;

1-PALMITOYL-2-OLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE; 1-PALMITOYL-2-OLEOYL-SN-GLYCERO-3-PHOSPHOSERINE; 3[(CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE; CANNABINOID RECEPTOR CB(2); CB(2); CHAPS; CHOLESTERYL HEMISUCCINATE TRIS SALT; CHS; DDM; DIETHYLENETRIAMINE PENTAACETATE; DTPA; FUNCTIONAL IMMOBILIZATION; G PROTEIN-COUPLED RECEPTOR; GPCR; MALTOSE BINDING PROTEIN; MBP; N-DODECYL-Β-D-MALTOSIDE; N-OCTYL-Β-D-GLUCOPYRANOSIDE; NITRILOTRIACETIC ACID; NTA; OG; PERIPHERAL CANNABINOID RECEPTOR; POPC; POPS; RHO-TAG; SPR; SURFACE PLASMON RESONANCE; SURFACE PLASMON RESONANCE (SPR); TEV; TOBACCO ETCH VIRUS;

ANTIBODIES, MONOCLONAL; DETERGENTS; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; IMMOBILIZED PROTEINS; KINETICS; LIGANDS; MICELLES; PROTEIN ARRAY ANALYSIS; RECEPTOR, CANNABINOID, CB2; RECOMBINANT FUSION PROTEINS; RHODOPSIN; THERMODYNAMICS;

EID: 84884476305     PISSN: 15709639     EISSN: 18781454     Source Type: Journal    
DOI: 10.1016/j.bbapap.2013.06.003     Document Type: Article

References (32)

1. 2 in immune regulation: therapeutic prospects for neuroinflammation Expert Rev. Mol. Med. 11 2009
2. 2 in detergent micelles and lipid bilayers PLoS One 7 2012
3. A. Yeliseev, L. Zoubak, and K. Gawrisch Use of dual affinity tags for expression and purification of functional peripheral cannabinoid receptor Protein Expr. Purif. 53 2007 153 163 (Pubitemid 46283465)
4. A.A. Yeliseev, K.K. Wong, O. Soubias, and K. Gawrisch Expression of human peripheral cannabinoid receptor for structural studies Protein Sci. 14 2005 2638 2653 (Pubitemid 41395590)
5. R.S. Molday, and D. MacKenzie Monoclonal antibodies to rhodopsin: characterization, cross-reactivity, and application as structural probes Biochemistry 22 1983 653 660
6. D.D. Oprian, R.S. Molday, R.J. Kaufman, and H.G. Khorana Expression of a synthetic bovine rhodopsin gene in monkey kidney cells Proc. Natl. Acad. Sci. U. S. A. 84 1987 8874 8878
7. M. Shimada, X. Chen, T. Cvrk, H. Hilfiker, M. Parfenova, and G.V. Segre Purification and characterization of a receptor for human parathyroid hormone and parathyroid hormone-related peptide J. Biol. Chem. 277 2002 31774 31780 (Pubitemid 34968982)
8. D.L. Farrens, T.D. Dunham, J.F. Fay, I.C. Dews, J. Caldwell, and B. Nauert Design, expression, and characterization of a synthetic human cannabinoid receptor and cannabinoid receptor/G-protein fusion protein J. Pept. Res. 60 2002 336 347 (Pubitemid 36071897)
9. T. Mirzabekov, N. Bannert, M. Farzan, W. Hofmann, P. Kolchinsky, L.J. Wu, R. Wyatt, and J. Sodroski Enhanced expression, native purification, and characterization of CCR5, a principal HIV-1 coreceptor J. Biol. Chem. 274 1999 28745 28750
10. K. Corin, P. Baaske, D.B. Ravel, J.Y. Song, E. Brown, X.Q. Wang, S. Geissler, C.J. Wienken, M. Jerabek-Willemsen, S. Duhr, D. Braun, and S.G. Zhang A robust and rapid method of producing soluble, stable, and functional G-protein coupled receptors PLoS One 6 2011
11. G.J. Babcock, T. Mirzabekov, W. Wojtowicz, and J. Sodroski Ligand binding characteristics of CXCR4 incorporated into paramagnetic proteoliposomes J. Biol. Chem. 276 2001 38433 38440
12. T. Mirzabekov, H. Kontos, M. Farzan, W. Marasco, and J. Sodroski Paramagnetic proteoliposomes containing a pure, native, and oriented seven-transmembrane segment protein, CCR5 Nat. Biotechnol. 18 2000 649 654 (Pubitemid 30415300)
13. P. Stenlund, G.J. Babcock, J. Sodroski, and D.G. Myszka Capture and reconstitution of G protein-coupled receptors on a biosensor surface Anal. Biochem. 316 2003 243 250 (Pubitemid 36444554)
14. I. Navratilova, J. Sodroski, and D.G. Myszka Solubilization, stabilization, and purification of chemokine receptors using biosensor technology Anal. Biochem. 339 2005 271 281 (Pubitemid 40395285)
15. R.L. Rich, A.R. Miles, B.K. Gale, and D.G. Myszka Detergent screening of a G-protein-coupled receptor using serial and array biosensor technologies Anal. Biochem. 386 2009 98 104
16. I. Navratilova, M. Pancera, R.T. Wyatt, and D.G. Myszka A biosensor-based approach toward purification and crystallization of G protein-coupled receptors Anal. Biochem. 353 2006 278 283 (Pubitemid 43775440)
17. I. Navratilova, M. Dioszegi, and D.G. Myszka Analyzing ligand and small molecule binding activity of solubilized GPCRs using biosensor technology Anal. Biochem. 355 2006 132 139 (Pubitemid 44041399)
18. I. Navratilova, J. Besnard, and A.L. Hopkins Screening for GPCR ligands using surface plasmon resonance ACS Med. Chem. Lett. 2 2011 549 554
19. E. Stenberg, B. Persson, H. Roos, and C. Urbaniczky Quantitative- determination of surface concentration of protein with surface plasmon resonance using radiolabeled proteins J. Colloid Interface Sci. 143 1991 513 526
20. 2 Protein Expr. Purif. 89 2013 62 72
21. D.R. Hall, and D.J. Winzor Use of a resonant mirror biosensor to characterize the interaction of carboxypeptidase A with an elicited monoclonal antibody Anal. Biochem. 244 1997 152 160 (Pubitemid 27030539)
22. 2 by bacterial fermentation Protein Expr. Purif. 70 2010 236 247
23. T. Kimura, A.A. Yeliseev, K. Vukoti, S.D. Rhodes, K. Cheng, K.C. Rice, and K. Gawrisch Recombinant cannabinoid type 2 receptor in liposome model activates G protein in response to anionic lipid constituents J. Biol. Chem. 287 2012 4076 4087
24. K.C. Min, Y. Jin, and W.A. Hendrickson Large-scale production of a disulfide-stabilized constitutively active mutant opsin Protein Expr. Purif. 75 2011 236 241
25. B.-K. Lee, K.-S. Jung, C. Son, H. Kini, N.C. VerBerknioes, B. Arshava, F. Naider, and J.M. Becker Affinity purification and characterization of a G-protein coupled receptor, Saccharomyces cerevisiae Ste2p Protein Expr. Purif. 56 2007 62 71 (Pubitemid 47499086)
26. L.S. Barak, K. Warabi, X. Feng, M.G. Caron, and M.M. Kwatra Real-time visualization of the cellular redistribution of G protein-coupled receptor kinase 2 and beta-arrestin 2 during homologous desensitization of the substance P receptor J. Biol. Chem. 274 1999 7565 7569
27. K. Corin, P. Baaske, D.B. Ravel, J. Song, E. Brown, X. Wang, S. Geissler, C.J. Wienken, M. Jerabek-Willemsen, S. Duhr, D. Braun, and S. Zhang A robust and rapid method of producing soluble, stable, and functional G-protein coupled receptors PLoS One 6 2011 e23036
28. R.S. Hodges, R.J. Heaton, J.M.R. Parker, L. Molday, and R.S. Molday Antigen-antibody interaction - synthetic peptides define linear antigenic determinants recognized by monoclonal antibodies directed to the cytoplasmic carboxyl terminus of rhodopsin J. Biol. Chem. 263 1988 11768 11775
29. G.J. Wegner, A.W. Wark, H.J. Lee, E. Codner, T. Saeki, S.P. Fang, and R.M. Corn Real-time surface plasmon resonance imaging measurements for the multiplexed determination of protein adsorption/desorption kinetics and surface enzymatic reactions on peptide microarrays Anal. Chem. 76 2004 5677 5684 (Pubitemid 39313313)
30. T.L. Hoffman, G. Canziani, L. Jia, J. Rucker, and R.W. Doms A biosensor assay for studying ligand-membrane receptor interactions: binding of antibodies and HIV-1 Env to chemokine receptors Proc. Natl. Acad. Sci. U. S. A. 97 2000 11215 11220
31. C. Yoshiura, Y. Kofuku, T. Ueda, Y. Mase, M. Yokogawa, M. Osawa, Y. Terashima, K. Matsushima, and I. Shimada NMR Analyses of the Interaction between CCR5 and Its Ligand Using Functional Reconstitution of CCR5 in Lipid Bilayers J. Am. Chem. Soc. 132 2010 6768 6777
32. M.A. O'Malley, M.E. Helgeson, N.J. Wagner, and A.S. Robinson Toward rational design of protein detergent complexes: determinants of mixed micelles that are critical for the in vitro stabilization of a G-protein coupled receptor Biophys. J. 101 2011 1938 1948