Large-scale production of a disulfide-stabilized constitutively active mutant opsin

Protein Expression and Purification

Volumn 75, Issue 2, 2011, Pages 236-241

  Min, K Christopher ^a   Jin, Yan ^a   Hendrickson, Wayne A ^a  

^a Och Spine at New York Presbyterian Hospitals   (United States)

Author keywords

Constitutive activation; G protein; GPCR; Receptor; Signaling

Indexed keywords

CYSTEINE; DETERGENT; DISULFIDE; OPSIN; RECOMBINANT PROTEIN; RETINAL; TRANSDUCIN;

ANIMAL; ARTICLE; CATTLE; CELL LINE; CHEMISTRY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; PROTEIN STABILITY; SIGNAL TRANSDUCTION; SITE DIRECTED MUTAGENESIS;

ANIMALS; CATTLE; CELL LINE; CLONING, MOLECULAR; CYSTEINE; DETERGENTS; DISULFIDES; HUMANS; MUTAGENESIS, SITE-DIRECTED; OPSINS; PROTEIN STABILITY; RECOMBINANT PROTEINS; RETINALDEHYDE; SIGNAL TRANSDUCTION; TRANSDUCIN;

MAMMALIA;

EID: 78649323070     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2010.09.011     Document Type: Article

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