Changes in the catalytic properties and substrate specificity of Bacillus sp. US149 maltogenic amylase by mutagenesis of residue 46

Journal of Industrial Microbiology and Biotechnology

Volumn 40, Issue 9, 2013, Pages 947-953

  Ben Mabrouk, Sameh ^a   Ayadi Zouari, Dorra ^a   Ben Hlima, Hajer ^a   Bejar, Samir ^a  

^a UNIVERSITY OF SFAX   (Tunisia)

Author keywords

Affinity; Catalytic efficiency; Kinetic parameters; Maltogenic amylase; Site directed mutagenesis

Indexed keywords

AMYLASE;

ARTICLE; BACILLUS; BINDING AFFINITY; BIOINFORMATICS; CATALYSIS; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STRUCTURE; ESCHERICHIA COLI; HYDROLYSIS; MOLECULAR DYNAMICS; MOLECULAR MODEL; MUTAGENESIS; NONHUMAN; PH; PHYSICAL CHEMISTRY; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY; WILD TYPE;

BACILLUS SP.;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ASPARTIC ACID; BACILLUS; BINDING SITES; BIOCATALYSIS; CATALYTIC DOMAIN; ENZYME STABILITY; GLYCOSIDE HYDROLASES; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 84884355563     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-013-1300-8     Document Type: Article

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