Molecular and enzymatic characterization of a maltogenic amylase that hydrolyzes and transglycosylates acarbose

European Journal of Biochemistry

Volumn 253, Issue 1, 1998, Pages 251-262

  Cha, Hyun Ju ^a   Yoon, Hyun Geun ^a   Kim, Young Wan ^a   Lee, Hee Seob ^a   Kim, Jung Wan ^b   Kweon, Ki Sung ^a   Oh, Byung Ha ^c   Park, Kwan Hwa ^a  

^a Seoul National University   (South Korea)

^b SolarLight Ltd   (South Korea)

^c Pohang University of Science and Technology (POSTECH)   (South Korea)

Author keywords

Acarbose; Bacillus stearothermophilus; Maltogenic amylase; Site directed mutagenesis; Transglycosylation

Indexed keywords

ACARBOSE; AMYLASE;

AMINO ACID SEQUENCE; ARTICLE; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ESCHERICHIA COLI; GEOBACILLUS STEAROTHERMOPHILUS; MOLECULAR CLONING; PRIORITY JOURNAL; PROTEIN EXPRESSION; SEQUENCE HOMOLOGY;

ACARBOSE; AMINO ACID SEQUENCE; AMYLASES; BACILLUS STEAROTHERMOPHILUS; BASE SEQUENCE; CLONING, MOLECULAR; CONSERVED SEQUENCE; DNA, BACTERIAL; ESCHERICHIA COLI; GENES, BACTERIAL; GLYCOSYLATION; HYDROLYSIS; MODELS, CHEMICAL; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; SEQUENCE HOMOLOGY, AMINO ACID; TRISACCHARIDES;

BACILLUS LICHENIFORMIS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; GEOBACILLUS STEAROTHERMOPHILUS; POSIBACTERIA; PROKARYOTA;

EID: 0032053544     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1998.2530251.x     Document Type: Article

References (50)

1. Takaku, H. (1988) Anomalously linked oligosaccharides mixture, in Handbook of amylases and related enzymes: their sources, isolation methods, properties and applications (The Amylase Research Society of Japan), pp. 215-217, Pergamon Press, New York.
2. Vihinen, M. & Mantsala, P. (1989) Microbial amylolytic enzymes, CRC Rev. Biochem. Mol. Biol. 24, 329-418.
3. Nakajima, R., Imanaka, T. & Aiba, S. (1986) Comparison of amino acid sequences of eleven different α-amylase, Appl. Microbiol. Biotechnol. 23, 355-360.
4. Bender, H. & Wallenfels, K. (1961) Untersuchungen an Pullulan. II Spezifischer Abbau durch ein bakterielles Enzym, Biochem. Z. 334, 79-95.
5. Matsumoto, A. & Matsuda, K. (1983) Role of branching enzyme in glycogen biosynthesis in Neurospora crassa, J. Jpn Soc. Starch Sci. 30, 212-222.
6. Hiroki, T., Okada, S. & Imanaka, T. (1992) Action of neopullula nase, J. Biol. Chem. 267, 18447-18452.
7. David, M. H., Gunther, H. & Roper, H. (1987) Catalytic properties of Bacillus megaterium amylase, Starch 39, 436-440.
8. Tonozuka, T., Ibuka, A., Sakai, H., Matsuzawav H. & Sakano, Y. (1996) Comparison of transglycosylation catalyzed by two α-amylases from Thermoactinomyces vulgaris R-47, J. Appl. Gly-cosci. 43, 95-98.
9. Kitahata, S., Tsuyama, N. & Okada, S. (1974) Purification and some properties of cyclodextrin glycosyltransferase from a strain of Bacillus species, Agric. Biol. Chem 38, 387-393.
10. Podkovyrov, S. M. & Zeikus, J. G. (1992) Structure of the gene encoding cylcomaltodextrinase from Clostridium thermohy-drosulfuricum 39E and characterization of the enzyme purified from Escherichia coli, J. Bacteriol. 174, 5400-5405.
11. Galvein, N. M. Kelly, C. T. & Fogarty, W. M (1994) Purification and properties of the cyclodextrinase of Bacillus sphaericus ATCC 7055, Appl. Microbiol. Biotechnol. 42, 46-50.
12. 8-barrel domain and evolutionary relationship to other amylolytic enzymes, J. Protein Chem. 12, 791-805.
13. Kim, I. C., Jang, S. Y., Cha, J. H., Ko, Y. H., Park, K. H. & Rho, H. M. (1988) Cloning and expression of thermostable alpha-amylase gene in Escherichia coli from Bacillus licheniformis ATCC 27811, Korean J. Appl. Microbiol. Bioeng. 16, 369-373.
14. Kim, I. C., Cha, J. H., Kim, J. R., Jang, S. Y., Seo, B. C., Cheong, T. K., Lee, D. S., Choi, Y. D. & Park, K. H. (1992) Catalytic properties of the cloned amylase from Bacillus licheniformis, J. Biol. Chem. 267, 22 108-22 114.
15. Shimizu, M., Kanno, M. Tamura, M. & Suekanne, M. (1978) Purification and some properties of a novel α-amylase of Thermoactinomyces vulgaris, Agric. Biol. Chem. 42, 1681-1688.
16. Sakano, Y Hiraiwa, S. I., Fukushima, J. & Kobayashi, T. (1982) Enzymatic Properties and Action Pattern of Thermoactynomyces vulgaris α-amylase, Agric. Biol. Chem. 46, 1121-1129.
17. Suzuki, Y. & Imai, T. (1985) Bacillus stearothermophilus KP 1064 pullulan hydrolase. Its assignment to a unique type of maltogenic α-amylase but to neither pullulanase nor isopullulanase. Appl. Microbiol. Biotechnol. 21, 20-26.
18. Imanaka, T. & Kurikiv, T. (1989) Pattern of action of Bacillus stearothermophilus neopullulanase on pullulan, J. Bacteriol. 171, 369-374.
19. Kuriki, T., Okada, S. & Imanaka, T. (1988) New type of pullulanase from Bacillus stearothermophilus and molecular cloning and expression of the gene in Bacillus subtilis, J. Bacteriol. 170, 1554-1559.
20. Kim, I. C., Yoo, S. H., Lee, S. J., Oh. B. H., Kim, J. W. & Park, K. H. (1994) Synthesis of branched oligosaccharides from starch by two amylases cloned from Bacillus licheniformis, Biosci. Biotechnol. Biochem. 58, 416-418.
21. Lee, S. J., Yoo, S. H., Kim, M. J., Kim, J. W. Seok, H. M. & Park, K. H. (1995) Production and characterization of branched oligosaccharides from liquefied starch by the action of B. licheniformis amylase, Starch. 47, 127-134.
22. DePinto, J. A. & Campbell, L. L. (1968) Purification and properties of the cyclodextrinase of Bacillus macerans, Biochemistry 7, 121-125.
23. Kitahata, S., Taniguchi, M., Beltran, S. D., Sugimoto, T. & Okada, S. (1983) Purification and some properties of cyclodextrinase form Bacillus coagulans, Agric. Binl, Chem. 47, 1441-1447.
24. Saha, B. C. & Zeikus, J. G. (1990) Characterization of thermostable cyclodextrinase from Clostridium thermohydrosulfuricum 39E, Appl. Environ. Microbiol. 56, 2941-2943.
25. Oguma, T., Matsuyama,A., Kikuchi, M. & Nakane, E. (1993) Cloning and sequence analysis of the cyclomaltodextrinase gene from Bacillus sphaericus and expression in Escherichia coli cells. Appl. Microbiol. Biotechnol. 39, 197-203.
26. Yoshida, A., Iwasaki, Y. Akiba, T. & Horikoshi, K. (1991) Purification and properties of cyclomaltodextrinase from alkalophilic Bacillus sp., J. Ferment. Bioeng. 71, 226-229.
27. Gibson, R. M. & Swensson, B. (1987) Identification of domains involved in binding of carbohydrate ligands to barley α-amylase 2. Carlsberg. Res. Commun. 52, 373-379.
28. Park, K., Yoon H., Cha, H. & Kim, J. (1997) Enzymic synthesis of branched oligosaccharidcs by transgenic B. stearothermophilus maltogenic amylase, in Engineering & food at ICEF 7 (Jowitt, R., ed.) pp. B13-B16, Sheffield Academic Press Ltd, Sheffield.
29. Dubnau, D. & Davidoff-Abenson, R. (1971) Fate of transforming DNA following uptake by competent Bacillus subtilis. I. Formation and properties of the donor-recipient complex, J. Mol. Biol. 56, 209-221.
30. Sanger, F., Nicklen, S. & Coulson, A. R. (1977) DNA sequencing with chain-termination inhibitors. Proc. Natl Acad. Sci. USA 74, 5463-5467.
31. Miller, C. L. (1959) Use of dinitrosalycylic acid reagent for determination of reducing sugar. Anal. Chem. 31, 426-428.
32. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
33. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227, 680-685.
34. Kunkel, T. A. (1985) Rapid and efficient site-specific mutagenesis without phenotypic selection, Proc. Natl Acad. Sci. USA 154, 488-492.
35. Kunkel, T. A., Roberts, J. D. & Zatour, R. A. (1987) Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 154, 367-382.
36. Kuriki, T. & Imanaka, T. (1989) Nucleotide sequence of the neopullulanase gene from Bacillus stearothermophilus, J. Gen. Microbiol. 135, 1521-1528.
37. Tonozuke, T., Ohtsuka, M., Mogi, S., Sakai, H., Ohta, T. & Sakano, Y. (1993) A neopullulanase-type α-amylase gene from Thermoactinomyces vulgaris R-47, Biosci. Biotechnol. Biochem. 57, 395-401.
38. Katsuragi, N.v Takizawa, N. & Murooka, Y. (1987) Entire nucleotide sequence of the pullulanase gene of Klebsiella aerogenes. J. Bacteriol. 169, 2301-2306.
39. Takano, T., Fukuda, M., Monma, M., Kobayashi, S., Kainuma, K. & Yamane, K. (1986) Molecular cloning. DNA nucleotide sequencing, and expression in Bacillus subtilis cells of the Bacillus macerans cyclodextrin glucanotransferase gene, J. Bacteriol. 166, 1118-1122.
40. Sakai, S., Kubota, M., Yamamoto, K., Nakada, T., Torigde, K., Ando, O. & Sugimoto, T. (1987) Cloning of cyclodextrin glucanotransferase genes from B. stearothermophilus and B. macerans, J. Jpn. Soc. Starch. Sci. 34, 140-147.
41. Gidley, M. J. (1985) Quantification of the structural features of starch polysaccharides by N. M. R. spectroseopy, Carbohydr. Res. 139, 85-93.
42. Kuriki, T., Kaneko, H., Yanase, M., Takata, H., Shimada, J., Handa, S., Takada, T., Umeyama, H. & Okada, S. (1996) Controlling substrate preference and transglycosylation activity of neopullulanase by manipulating steric constraint and hydrophobicity in active center. J. Biol. Chem. 271, 17321-17329.
43. Kyte, J. & Doolittle, R. F. (1982) A simple method for displaying the hydropathic character of a protein, J. Mol. Biol. 157, 105-132.
44. Matsuura, Y., Kusunoki, M., Harada, W. & Kakudo, M. (1984) Structure and possible catalytic residues of Taka-amylase A, J. Biochem. (Tokyo) 95, 697-702.
45. Buisson. G., Duee, E., Haser, R. & Payan, F. (1987) Three-dimensional structure of porcine pancreatine α-amylase at 2.9 Å resolution. EMBO J. 6, 3909-3916.
46. Takase, K. (1992) Interaction of catalytic-site mutants of Bacillus α-amylase with substrates and acarbose, Biochim. Biophys. Acta 1122, 278-282.
47. Wiocox, E. R. & Whitaker, J. R. (1984) Some aspects of the mechanism of complexation of red kidney bean α-amylase inhibitor and α-amylase, Biochemistry 23, 1783-1791.
48. Brzozowski, A. M. & Davits, G. J. (1997) Structure of the Aspergillus oryzae α-amylase complexed with the inhibitor acarbose at 2.0 Å resolution, Biochemistry 36, 10837-10845.
49. Jespersen, H. M., MacGregor, E. A., Sierks, M. R. & Svensson, B. (1991) Comparison of the domain-level organization of starch hydrolases and related enzymes, Biochem. J. 280, 51-55.
50. Diderichsen, B. & Christiansen, L. (1988) Cloning of a maltogenic alpha-amylase from Bacillus stearothermophilus, FEMS Microbiol. Lett. 56, 53-60.