Cloning and sequencing of an original gene encoding a maltogenic amylase from Bacillus sp. US149 strain and characterization of the recombinant activity

Molecular Biotechnology

Volumn 38, Issue 3, 2008, Pages 211-219

  Mabrouk, Sameh Ben ^a   Messaoud, Ezzedine Ben ^a   Ayadi, Dorra ^a   Jemli, Sonia ^a   Roy, Amitava ^a   Mezghani, Monia ^a   Bejar, Samir ^a  

^a CENTRE DE BIOTECHNOLOGIE DE SFAX   (Tunisia)

Author keywords

Cyclodextrinase; Inverse PCR; Kinetic parameters; Maltogenic amylase; Purification; Sequence homology

Indexed keywords

AMINES; AMINO ACIDS; BACTERIOLOGY; CLONING; ENCODING (SYMBOLS); ENZYMES; ESCHERICHIA COLI; EXTRACTION; FOOD ADDITIVES; GENETIC ENGINEERING; GLUCOSE; MALTOSE; NUCLEIC ACIDS; ORGANIC ACIDS;

AMINO-ACID SEQUENCES; BACILLUS SP.; CRUDE EXTRACTS; CYCLO DEXTRIN; CYCLODEXTRINASE; INVERSE PCR; KINETIC PARAMETERS; MALTOGENIC AMYLASE; MALTOGENIC AMYLASES; NUCLEOTIDE SEQUENCE ANALYSIS; OPEN READING FRAME; PAGE ANALYSIS; PULLULAN; PURIFICATION; RECOMBINANT ENZYMES; REDUCING CONDITIONS; SEQUENCE HOMOLOGY;

GENE ENCODING;

AMYLASE; BETA CYCLODEXTRIN; CARBOHYDRATE; GLUCOSE; MALTOGENIC AMYLASE; MALTOSE; PANOSE; PULLULAN; STARCH; BACTERIAL PROTEIN; GLUCAN 1,4 ALPHA MALTOHYDROLASE; GLUCAN 1,4-ALPHA-MALTOHYDROLASE; GLYCOSIDASE;

AMINO ACID SEQUENCE; ARTICLE; BACILLUS; CELLULAR DISTRIBUTION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME LOCALIZATION; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE OVEREXPRESSION; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; TEMPERATURE; DNA SEQUENCE; ENZYME ACTIVATION; ENZYME STABILITY; ENZYMOLOGY; GENETICS; HYDROLYSIS; METABOLISM; MOLECULAR GENETICS; THIN LAYER CHROMATOGRAPHY;

BACILLUS SP.; ESCHERICHIA COLI; THERMUS SP.;

AMINO ACID SEQUENCE; BACILLUS; BACTERIAL PROTEINS; CHROMATOGRAPHY, THIN LAYER; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ACTIVATION; ENZYME STABILITY; GLYCOSIDE HYDROLASES; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; MALTOSE; MOLECULAR SEQUENCE DATA; OPEN READING FRAMES; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; TEMPERATURE;

EID: 44449090676     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-007-9017-4     Document Type: Article

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