A new thermoactive pullulanase from Desulfurococcus mucosus: Cloning, sequencing, purification, and characterization of the recombinant enzyme after expression in Bacillus subtilis

Journal of Bacteriology

Volumn 182, Issue 22, 2000, Pages 6331-6338

  Duffner, F ^a   Bertoldo, C ^a   Andersen, J T ^a   Wagner, K ^a   Antranikian, G ^a  

^a HAMBURG UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

PULLULANASE; RECOMBINANT ENZYME;

ARTICLE; BACILLUS SUBTILIS; DESULFUROCOCCUS; ENZYME ANALYSIS; ENZYME DEGRADATION; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; THERMOSTABILITY;

AMINO ACID SEQUENCE; ARCHAEAL PROTEINS; BACILLUS SUBTILIS; BACTERIAL PROTEINS; CLONING, MOLECULAR; CONSERVED SEQUENCE; CYCLODEXTRINS; DESULFUROCOCCACEAE; GENES, BACTERIAL; GLUCANS; GLYCOSIDE HYDROLASES; HYDROGEN-ION CONCENTRATION; MALTOSE; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; STARCH; SUBSTRATE SPECIFICITY; TEMPERATURE; TRISACCHARIDES;

EID: 0033760418     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.182.22.6331-6338.2000     Document Type: Article

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