Detection of muramyl dipeptide-sensing pathway defects in monocytes of patients with Crohn's disease using phospho-specific whole blood flow cytometry

Scandinavian Journal of Clinical and Laboratory Investigation

Volumn 73, Issue 6, 2013, Pages 494-502

  Kuuliala, Krista ^a   Lappalainen, Maarit ^b   Turunen, Ulla ^b   Puolakkainen, Pauli ^b   Kemppainen, Esko ^b   Siitonen, Sanna ^c   Repo, Heikki ^a   Mustonen, Harri ^b  

^a UNIVERSITY OF HELSINKI   (Finland)

^b Department of Medicine ^*  (Finland)

^c HELSINKI UNIVERSITY CENTRAL HOSPITAL   (Finland)

Author keywords

CARD15 mutations; Clinical screening; Leukocyte signaling

Indexed keywords

BACTERIUM LIPOPOLYSACCHARIDE; CASPASE RECRUITMENT DOMAIN PROTEIN 15; FLUORESCEIN ISOTHIOCYANATE; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; MITOGEN ACTIVATED PROTEIN KINASE P38; MONOCLONAL ANTIBODY; MURAMYL DIPEPTIDE; PHOSPHOSPECIFIC ANTIBODY;

ADULT; ALLELE; ANTIBODY DETECTION; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; CROHN DISEASE; ENZYME LINKED IMMUNOSORBENT ASSAY; EX VIVO STUDY; FEMALE; FLOW CYTOMETRY; GENE MUTATION; HETEROZYGOTE; HOMOZYGOTE; HUMAN; HUMAN CELL; HUMAN TISSUE; LEUKOCYTE DIFFERENTIAL COUNT; MALE; MONOCYTE; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; WESTERN BLOTTING;

ACETYLMURAMYL-ALANYL-ISOGLUTAMINE; ADULT; AGED; CASE-CONTROL STUDIES; CROHN DISEASE; FEMALE; FLOW CYTOMETRY; HUMANS; LEUKOCYTES, MONONUCLEAR; MALE; MIDDLE AGED; MUTATION, MISSENSE; NF-KAPPA B; NOD2 SIGNALING ADAPTOR PROTEIN; P38 MITOGEN-ACTIVATED PROTEIN KINASES; PHOSPHOPROTEINS; PHOSPHORYLATION; PROTEIN PROCESSING, POST-TRANSLATIONAL; SIGNAL TRANSDUCTION;

EID: 84884260562     PISSN: 00365513     EISSN: 15027686     Source Type: Journal    
DOI: 10.3109/00365513.2013.811612     Document Type: Article

References (26)

1. Marks DJ , Harbord MW , MacAllister R , Rahman FZ, Young J , Al-Lazikani B , Lees W , Novelli M , Bloom S , Segal AW . Defective acute infl ammation in Crohn s disease: a clinical investigation . Lancet 2006 ; 367 : 668-78 . (Pubitemid 43289157)
2. P l sson-McDermott E M , O Neill L A . Signal transduction by the lipopolysaccharide receptor, Toll-like receptor 4 . Immunology 2004 ; 113 : 153-62 . (Pubitemid 39312442)
3. Girardin S E , Boneca I G , Viala J , Chamaillard M , Labigne A , Thomas G , Philpott DJ , Sansonetti PJ . Nod2 is a general sensor of peptidoglycan through muramyl dipeptide (MDP) detection . J Biol Chem 2003 ; 278 : 8869-72 . (Pubitemid 36800360)
4. Kanneganti TD , Lamkanfi M , Nnez G . Intracellular NOD-like receptors in host defense and disease . Immunity 2007 ; 27 : 549-59 . (Pubitemid 47615512)
5. Kobayashi K , Inohara N , Hernandez L D , Galn J E, Nnez G , Janeway CA , Medzhitov R , Flavell RA . RICK/ Rip2/CARDIAK mediates signaling for receptors of the innate and adaptive immune systems . Nature 2002 ; 416 : 194-9 . (Pubitemid 34246479)
6. Hsu Y M , Zhang Y , You Y , Wang D , Li H , Duramad O , Qin XF , Dong C , Lin X . The adaptor protein CARD9 is required for innate immune responses to intracellular pathogens . Nat Immunol 2007 ; 8 : 198-205 . (Pubitemid 46152651)
7. Hugot J P , Chamaillard M , Zouali H , Lesage S , C zard J P , Belaiche J , Almer S , Tysk C , O Morain CA , Gassull M , Binder V , Finkel Y , Cortot A , Modigliani R , Laurent-Puig P , Gower-Rousseau C , Macry, Colombel JF , Sahbatou M , Thomas G . Association of NOD2 leucine-rich repeat variants with susceptibility to Crohn s disease . Nature 2001 ; 411 : 599-603 (Pubitemid 32531408)
8. , Bonen DK , Inohara N , Nicolae DL , Chen FF, Ramos R , Britton H , Moran T , Karaliuskas R , Duerr RH , Achkar JP , Brant SR , Bayless TM , Kirschner BS , Hanauer SB , Nunez G , Cho JH . A frameshift mutation in NOD2 associated with susceptibility to Crohn s disease . Nature 2001 ; 411 : 603-6 . (Pubitemid 32531409)
9. Li J, Moran T , Swanson E, Julian C, Harris J, Bonen DK , Hedl M , Nicolae DL , Abraham C , Cho JH . Regulation of IL-8 and IL-1beta expression in Crohn s disease associated NOD2/CARD15 mutations . Hum Mol Genet 2004 ; 13 : 1715-25 . (Pubitemid 39173441)
10. van Heel DA , Ghosh S , Butler M , Hunt KA , Lundberg AM , Ahmad T , McGovern DP , Onnie C , Negoro K , Goldthorpe S , Foxwell BM , Mathew CG , Forbes A , Jewell DP , Playford RJ . Muramyl dipeptide and toll-like receptor sensitivity in NOD2-associated Crohn s disease . Lancet 2005 ; 365 : 1794-6 . (Pubitemid 40744660)
11. Kramer M , Netea MG , de Jong DJ , Kullberg B J, Adema GJ . Impaired dendritic cell function in Crohn s disease patients with NOD2 3020insC mutation . J Leukoc Biol 2006 ; 79 : 860-6 . (Pubitemid 44835619)
12. Netea M G , Ferwerda G , de Jong D J , Werts C , Boneca IG , Jhanno M , Van Der Meer JW , Mengin-Lecreulx D , Sansonetti PJ , Philpott DJ , Dharancy S , Girardin SE . The frameshift mutation in Nod2 results in unresponsiveness not only to Nod2- but also Nod1-activating peptidoglycan agonists . J Biol Chem 2005 ; 280 : 35859-67 . (Pubitemid 41633853)
13. Netea M G , Ferwerda G , de Jong D J , Jansen T , Jacobs L , Kramer M , Naber TH , Drenth JP , Girardin SE , Kullberg BJ , Adema GJ , Van Der Meer JW . Nucleotide-binding oligomerization domain-2 modulates specifi c TLR pathways for the induction of cytokine release . J Immunol 2005 ; 174 : 6518-23 . (Pubitemid 40663847)
14. Maeda S , Hsu LC, Liu H, Bankston LA , Iimura M , Kagnoff MF , Eckmann L , Karin M . Nod2 mutation in Crohn s disease potentiates NF- B activity and IL-1 processing . Science 2005 ; 307 : 734-8 . (Pubitemid 40194645)
15. Stjernberg-Salmela S , Ranki A , Karenko L , Siitonen S , Mustonen H , Puolakkainen P , Sarna S , Pettersson T , Repo H . Low TNF-induced NF-kappaB and p38 phosphorylation levels in leucocytes in tumour necrosis factor receptor- associated periodic syndrome . Rheumatology 2010 ; 49 : 382-90 .
16. Oiva J , Mustonen H , Kylnp M L , Kyhl L , Alanr T , Aittom ki S , Siitonen S , Kemppainen E , Puolakkainen P , Repo H . Patients with acute pancreatitis complicated by organ failure show highly aberrant monocyte signaling profi les assessed by phospho-specifi c fLow cytometry . Crit Care Med 2010 ; 38 : 1702-8 .
17. Oiva J , Mustonen H , Kylnp M L , Kyhl L , Kuuliala K , Siitonen S , Kemppainen E , Puolakkainen P , Repo H . Acute pancreatitis with organ dysfunction associates with abnormal blood lymphocyte signaling: controlled laboratory study . Crit Care 2010 ; 14 : R207 .
18. L appalainen M , Paavola-Sakki P , Halme L , Turunen U , Frkki M , Repo H , Kontula K . Novel CARD15/NOD2 mutations in Finnish patients with Crohn s disease and their relation to phenotypic variation in vitro and in vivo . Infl amm Bowel Dis 2008 ; 14 : 176-85 . (Pubitemid 351288038)
19. Seidelin J B, Broom O J, Olsen J , Nielsen O H . E vidence for impaired CARD15 signalling in Crohn s disease without disease linked variants . PLoS One 2009 ; 4 : e7794 .
20. Billmann-Born S , Till A , Arlt A , Lipinski S , Sina C, Latiano A , Annese V , Hsler R , Kerick M , Manke T , Seegert D , Hanidu A , Schfer H , van Heel D , Li J , Schreiber S , Rosenstiel P . Genome-wide expression profi ling identifi es an impairment of negative feedback signals in the Crohn s disease-associated NOD2 variant L1007fsinsC . J Immunol 2011 ; 186 : 4027-38
21. Vakkila J , Nieminen U , Siitonen S , Turunen U , Halme L , Nuutinen H , Mustonen H , Puolakkainen P , Frkki M , Repo H . A novel modifi cation of a fLow cytometric assay of phosphorylated STAT1 in whole blood monocytes for immunomonitoring of patients on IFN alpha regimen . Scand J Immunol 2008 ; 67 : 95-102 . (Pubitemid 350209579)
22. Turner M , Chernajovsky Y. Comment on: Low TNFinduced NF- B and p38 phosphorylation levels in leucocytes in tumour necrosis factor receptor-associated periodic syndrome [Letter] . Rheumatology 2011 ; 50 : 1525-6 .
23. Repo H, Jansson SE , Leirisalo-Repo M . A nticoagulant selection infl uences fLow cytometric determination of CD11b upregulation in vivo and ex vivo . J Immunol Methods 1995 ; 185 : 65-79 .
24. Newhall K J, Diemer G S , Leshinsky N , Kerkof K , Chute HT , Russell CB , Rees W , Welcher AA , Patterson SD , Means GD . Evidence for endotoxin contamination in plastic Na -heparin blood collection tube lots . Clin Chem 2010 ; 56 : 1483-91 .
25. McGregor RJ , Mole DJ . Once bitten, twice shy: defective monocyte signaling in acute pancreatitis [Editorial] . Crit Care Med 2010 ; 38 : 1751-2.
26. Caldwell CC, Hotchkiss RS . The fi rst step in utilizing immune-modulating therapies: immune status determination [Editorial] . Crit Care 2011 ; 15 : 108 .