DNA detection of Clostridium difficile infection based on real-time resistance measurement

Genetics and Molecular Research

Volumn 12, Issue 3, 2013, Pages 3296-3304

  Liu, C ^a   Jiang, D N ^a   Xiang, G M ^a   Luo, F K ^a   Liu, L L ^a   Yu, J C ^a   Pu, X Y ^a  

^a THIRD MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

Clostridium difficile; DNA detection; Loop mediated isothermal amplification; Real time resistance measurement

Indexed keywords

CRYSTAL VIOLET; DNA; MAGNESIUM; PYROPHOSPHATE;

ARTICLE; BACTERIAL GENE; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM DIFFICILE INFECTION; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST ACCURACY STUDY; DNA DETERMINATION; ELECTROCHEMICAL DETECTION; FECES ANALYSIS; HUMAN; INTERMETHOD COMPARISON; LIMIT OF DETECTION; LOOP MEDIATED ISOTHERMAL AMPLIFICATION; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; RAPID TEST; REAL TIME RESISTANCE MEASUREMENT; SENSITIVITY AND SPECIFICITY; TCDA GENE;

CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM INFECTIONS; DNA, BACTERIAL; FECES; HUMANS; LIMIT OF DETECTION; SENSITIVITY AND SPECIFICITY;

EID: 84884255511     PISSN: None     EISSN: 16765680     Source Type: Journal    
DOI: 10.4238/2013.September.3.6     Document Type: Article

References (25)

1. Abdul-Ghani R, Al-Mekhlafi AM and Karanis P (2012). Loop-mediated isothermal amplification (LAMP) for malarial parasites of humans: would it come to clinical reality as a point-of-care test? Acta Trop. 122: 233-240.
2. Becker CA, Ramos F, Sellal E, Moine S, et al. (2012). Development of a multiplex real-time PCR for contagious agalactia diagnosis in small ruminants. J. Microbiol. Methods 90: 73-79.
3. Chen N and Shih SL (2011). Images in clinical medicine. Pseudomembranous colitis. N. Engl. J. Med. 364: e8.
4. Cohen SH, Gerding DN, Johnson S, Kelly CP, et al. (2010). Clinical practice guidelines for Clostridium difficile infection in adults: 2010 update by the society for healthcare epidemiology of America (SHEA) and the infectious diseases society of America (IDSA). Infect. Control Hosp. Epidemiol. 31: 431-455.
5. Demidenko NV and Penin AA (2012). Comparative analysis of gene expression level by quantitative real-time PCR has limited application in objects with different morphology. PLoS One 7: e38161.
6. Eastwood K, Else P, Charlett A and Wilcox M (2009). Comparison of nine commercially available Clostridium difficile toxin detection assays, a real-time PCR assay for C. difficile tcdB, and a glutamate dehydrogenase detection assay to cytotoxin testing and cytotoxigenic culture methods. J. Clin. Microbiol. 47: 3211-3217.
7. Han HJ, Jung SJ, Oh MJ and Kim DH (2011). Rapid and sensitive detection of Streptococcus iniae by loop-mediated isothermal amplification (LAMP). J. Fish Dis. 34: 395-398.
8. Iseki H, Alhassan A, Ohta N, Thekisoe OM, et al. (2007). Development of a multiplex loop-mediated isothermal amplification (mLAMP) method for the simultaneous detection of bovine Babesia parasites. J. Microbiol. Methods 71: 281-287.
9. Jiang DN, Xiang GM, Wu JH, Liu C, et al. (2012). A Real-time resistance measurement for DNA amplification and detection. Int. J. Electrochem. Sc. 7: 5273-5285.
10. Le Roux CA, Kubo T, Grobbelaar AA, van Vuren PJ, et al. (2009). Development and evaluation of a real-time reverse transcription-loop-mediated isothermal amplification assay for rapid detection of Rift Valley fever virus in clinical specimens. J. Clin. Microbiol. 47: 645-651.
11. Mori Y, Kitao M, Tomita N and Notomi T (2004). Real-time turbidimetry of LAMP reaction for quantifying template DNA. J. Biochem. Biophys. Methods 59: 145-157.
12. Nagatani N, Yamanaka K, Ushijima H, Koketsu R, et al. (2012). Detection of influenza virus using a lateral flow immunoassay for amplified DNA by a microfluidic RT-PCR chip. Analyst 137: 3422-3426.
13. Nogami H, Tsutsumi H, Komuro T and Mukoyama R (2008). Rapid and simple sex determination method from dental pulp by loop-mediated isothermal amplification. Forensic Sci. Int. Genet. 2: 349-353.
14. Notomi T, Okayama H, Masubuchi H, Yonekawa T, et al. (2000). Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 28: E63.
15. Paris DH, Imwong M, Faiz AM, Hasan M, et al. (2007). Loop-mediated isothermal PCR (LAMP) for the diagnosis of falciparum malaria. Am. J. Trop. Med. Hyg. 77: 972-976.
16. Paris DH, Blacksell SD, Nawtaisong P, Jenjaroen K, et al. (2011). Diagnostic accuracy of a loop-mediated isothermal PCR assay for detection of Orientia tsutsugamushi during acute Scrub Typhus infection. PLoS Negl. Trop. Dis. 5: e1307.
17. Planche T, Aghaizu A, Holliman R, Riley P, et al. (2008). Diagnosis of Clostridium difficile infection by toxin detection kits: a systematic review. Lancet Infect. Dis. 8: 777-784.
18. Schwan C, Stecher B, Tzivelekidis T, van HM, et al. (2009). Clostridium difficile toxin CDT induces formation of microtubule-based protrusions and increases adherence of bacteria. PLoS Pathog. 5: e1000626.
19. Sowmya N, Thakur MS and Manonmani HK (2012). Rapid and simple DNA extraction method for the detection of enterotoxigenic Staphylococcus aureus directly from food samples: comparison of PCR and LAMP methods. J. Appl. Microbiol. 113: 106-113.
20. Tenover FC, Novak-Weekley S, Woods CW, Peterson LR, et al. (2010). Impact of strain type on detection of toxigenic Clostridium difficile: comparison of molecular diagnostic and enzyme immunoassay approaches. J. Clin. Microbiol. 48: 3719-3724.
21. Tjong V, Yu H, Hucknall A, Rangarajan S, et al. (2011). Amplified on-chip fluorescence detection of DNA hybridization by surface-initiated enzymatic polymerization. Anal Chem. 83: 5153-5159.
22. Tomita N, Mori Y, Kanda H and Notomi T (2008). Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products. Nat. Protoc. 3: 877-882.
23. Wang X, Zhu JP, Zhang Q, Xu ZG, et al. (2012). Detection of enterovirus 71 using reverse transcription loop-mediated isothermal amplification (RT-LAMP). J. Virol. Methods 179: 330-334.
24. Wilkins TD and Lyerly DM (2003). Clostridium difficile testing: after 20 years, still challenging. J. Clin. Microbiol. 41: 531-534.
25. Wu J, Kodzius R, Xiao K, Qin J, et al. (2012). Fast detection of genetic information by an optimized PCR in an interchangeable chip. Biomed. Microdevices. 14: 179-186.