Impact of strain type on detection of toxigenic Clostridium difficile: Comparison of molecular diagnostic and enzyme immunoassay approaches

Journal of Clinical Microbiology

Volumn 48, Issue 10, 2010, Pages 3719-3724

  Tenover, Fred C ^a   Novak Weekley, Susan ^b   Woods, Christopher W ^c   Peterson, Lance R ^d   Davis, Thomas ^e   Schreckenberger, Paul ^f   Fang, Ferric C ^g   Dascal, Andre ^h   Gerding, Dale N ^f,i   Nomura, Jim H ^b   Goering, Richard V ^j   Akerlund, Thomas ^k   Weissfeld, Alice S ^l   Baron, Ellen Jo ^a,m   Wong, Edith ^a   Marlowe, Elizabeth M ^b   Whitmore, Joseph ^a   Persing, David H ^a  

^a CEPHEID   (United States)

^b KAISER PERMANENTE   (United States)

^c DURHAM VA MEDICAL CENTER   (United States)

^d NORTHSHORE UNIVERSITY HEALTHSYSTEM   (United States)

^e WISHARD MEMORIAL HOSPITAL   (United States)

^f LOYOLA UNIVERSITY MEDICAL CENTER   (United States)

^g UNIVERSITY OF WASHINGTON   (United States)

^h MCGILL UNIVERSITY   (Canada)

ⁱ HINES VA HOSPITAL   (United States)

^j CREIGHTON UNIVERSITY MEDICAL CENTER   (United States)

^k SWEDISH INSTITUTE FOR INFECTIOUS DISEASE CONTROL   (Sweden)

^l Microbiology Specialists Incorporated   (United States)

^m STANFORD UNIVERSITY   (United States)

more..

Author keywords

[No Author keywords available]

Indexed keywords

GLUTAMATE DEHYDROGENASE;

ALGORITHM; ARTICLE; BACTERIAL STRAIN; BACTERIUM DETECTION; BACTERIUM ISOLATE; CELL CULTURE; CLOSTRIDIUM DIFFICILE; CONTROLLED STUDY; CYTOTOXICITY; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; ENZYME ASSAY; ENZYME IMMUNOASSAY; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SCREENING;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; BACTERIAL TYPING TECHNIQUES; BACTERIOLOGICAL TECHNIQUES; CHILD; CHILD, PRESCHOOL; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM INFECTIONS; FECES; FEMALE; HUMANS; IMMUNOENZYME TECHNIQUES; MALE; MIDDLE AGED; MOLECULAR DIAGNOSTIC TECHNIQUES; RIBOTYPING; SENSITIVITY AND SPECIFICITY; TOXICITY TESTS; YOUNG ADULT;

CLOSTRIDIUM DIFFICILE;

EID: 77957773862     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00427-10     Document Type: Article

References (36)

1. Alcalá, L., L. Sanchez-Cambronero, M. P. Catalan, M. Sanchez-Somolinos, M. T. Pelaez, M. Marin, and E. Bouza. 2008. Comparison of three commercial methods for rapid detection of Clostridium difficile toxins A and B from fecal specimens. J. Clin. Microbiol. 46:3833-3835.
2. Barbut, F., P. Mastrantonio, M. Delmee, J. Brazier, E. Kuijper, and I. Poxton. 2007. Prospective study of Clostridium difficile infections in Europe with phenotypic and genotypic characterisation of the isolates. Clin. Microbiol. Infect. 13:1048-1057.
3. Cardona, D. M., and K. H. Rand. 2008. Evaluation of repeat Clostridium difficile enzyme immunoassay testing. J. Clin. Microbiol. 46:3686-3689.
4. Doing, K. M., M. S. Hintz, C. Keefe, S. Horne, S. Levasseur, and M. L. Kulikowski. 2010. Reevaluation of the Premier Clostridium difficile toxin A and B immunoassay with comparison to glutamate dehydrogenase common antigen testing evaluating Bartels cytotoxin and Prodesse ProGastro Cd polymerase chain reaction as confirmatory procedures. Diagn. Microbiol. Infect. Dis. 66:129-134.
5. Eastwood, K., P. Else, A. Charlett, and M. Wilcox. 2009. Comparison of nine commercially available Clostridium difficile toxin detection assays, a real-time PCR assay for C. difficile tcdB, and a glutamate dehydrogenase detection assay to cytotoxin testing and cytotoxigenic culture methods. J. Clin. Microbiol. 47:3211-3217.
6. Gilligan, P. H. 2008. Is a two-step glutamate dehyrogenase antigen-cytotoxicity neutralization assay algorithm superior to the Premier toxin A and B enzyme immunoassay for laboratory detection of Clostridium difficile? J. Clin. Microbiol. 46:1523-1525.
7. Gumerlock, P. H., Y. J. Tang, J. B. Weiss, and J. Silva, Jr. 1993. Specific detection of toxigenic strains of Clostridium difficile in stool specimens. J. Clin. Microbiol. 31:507-511.
8. Huang, H., A. Weintraub, H. Fang, and C. E. Nord. 2009. Comparison of a commercial multiplex real-time PCR to the cell cytotoxicity neutralization assay for diagnosis of Clostridium difficile infections. J. Clin. Microbiol. 47:3729-3731.
9. Johnson, S., M. H. Samore, K. A. Farrow, G. E. Killgore, F. C. Tenover, D. Lyras, J. I. Rood, P. DeGirolami, A. L. Baltch, M. E. Rafferty, S. M. Pear, and D. N. Gerding. 1999. Epidemics of diarrhea caused by a clindamycin-resistant strain of Clostridium difficile in four hospitals. N. Engl. J. Med. 341:1645-1651.
10. Kallen, A. J., A. Thompson, P. Ristaino, L. Chapman, A. Nicholson, B. T. Sim, F. Lessa, U. Sharapov, E. Fadden, R. Boehler, C. Gould, B. Limbago, D. Blythe, and L. C. McDonald. 2009. Complete restriction of fluoroquinolone use to control an outbreak of Clostridium difficile infection at a community hospital. Infect. Control Hosp. Epidemiol. 30:264-272.
11. Kelly, C. P., and J. T. LaMont. 2008. Clostridium difficile - more difficult than ever. N. Engl. J. Med. 359:1932-1940.
12. Kuijper, E. J., B. Coignard, J. S. Brazier, C. Suetens, D. Drudy, C. Wiuff, H. Pituch, P. Reichert, F. Schneider, A. F. Widmer, K. E. Olsen, F. Allerberger, D. W. Notermans, F. Barbut, M. Delmee, M. Wilcox, A. Pearson, B. C. Patel, D. J. Brown, R. Frei, T. Akerlund, I. R. Poxton, and P. Tull. 2007. Update of Clostridium difficile-associated disease due to PCR ribotype 027 in Europe. Euro Surveill. 12:E1-E2.
13. Kuijper, E. J., B. Coignard, and P. Tull. 2006. Emergence of Clostridium difficile-associated disease in North America and Europe. Clin. Microbiol. Infect. 12(Suppl. 6):2-18.
14. Kutty, P. K., S. R. Benoit, C. W. Woods, A. C. Sena, S. Naggie, J. Frederick, J. Engemann, S. Evans, B. C. Pien, S. N. Banerjee, J. Engel, and L. C. McDonald. 2008. Assessment of Clostridium difficile-associated disease surveillance definitions, North Carolina, 2005. Infect. Control Hosp. Epidemiol. 29:197-202.
15. Larson, A. M., A. M. Fung, and F. C. Fang. 2010. Evaluation of tcdB real-time PCR in a three-step diagnostic algorithm for detection of toxigenic Clostridium difficile. J. Clin. Microbiol. 48:124-130.
16. Limbago, B. M., C. M. Long, A. D. Thompson, G. E. Killgore, G. E. Hannett, N. L. Havill, S. Mickelson, S. Lathrop, T. F. Jones, M. M. Park, K. H. Harriman, L. H. Gould, L. C. McDonald, and F. J. Angulo. 2009. Clostridium difficile strains from community-associated infections. J. Clin. Microbiol. 47:3004-3007.
17. Lyerly, D. M., L. A. Barroso, and T. D. Wilkins. 1991. Identification of the latex test-reactive protein of Clostridium difficile as glutamate dehydrogenase. J. Clin. Microbiol. 29:2639-2642.
18. Lyerly, D. M., and T. D. Wilkins. 1986. Commercial latex test for Clostridium difficile toxin A does not detect toxin A. J. Clin. Microbiol. 23:622-623.
19. Lyras, D., J. R. O'Connor, P. M. Howarth, S. P. Sambol, G. P. Carter, T. Phumoonna, R. Poon, V. Adams, G. Vedantam, S. Johnson, D. N. Gerding, and J. I. Rood. 2009. Toxin B is essential for virulence of Clostridium difficile. Nature 458:1176-1179.
20. McDonald, L. C., G. E. Killgore, A. Thompson, R. C. Owens, Jr., S. V. Kazakova, S. P. Sambol, S. Johnson, and D. N. Gerding. 2005. An epidemic, toxin gene-variant strain of Clostridium difficile. N. Engl. J. Med. 353:2433-2441.
21. McFarland, L. V. 2002. What's lurking under the bed? Persistence and predominance of particular Clostridium difficile strains in a hospital and the potential role of environmental contamination. Infect. Control Hosp. Epidemiol. 23:639-640.
22. Muto, C. A., M. K. Blank, J. W. Marsh, E. N. Vergis, M. M. O'Leary, K. A. Shutt, A. W. Pasculle, M. Pokrywka, J. G. Garcia, K. Posey, T. L. Roberts, B. A. Potoski, G. E. Blank, R. L. Simmons, P. Veldkamp, L. H. Harrison, and D. L. Paterson. 2007. Control of an outbreak of infection with the hypervirulent Clostridium difficile BI strain in a university hospital using a comprehensive "bundle" approach. Clin. Infect. Dis. 45:1266-1273.
23. Novak-Weekley, S. M., E. M. Marlowe, J. M. Miller, J. Cumpio, J. H. Nomura, P. H. Vance, and A. Weissfeld. 2010. Clostridium difficile testing in the clinical laboratory using multiple testing algorithms. J. Clin. Microbiol. 48:889-893.
24. Peterson, L. R., R. U. Manson, S. M. Paule, D. M. Hacek, A. Robicsek, R. B. Thomson, Jr., and K. L. Kaul. 2007. Detection of toxigenic Clostridium difficile in stool samples by real-time polymerase chain reaction for the diagnosis of C. difficile-associated diarrhea. Clin. Infect. Dis. 45:1152-1160.
25. Peterson, L. R., and A. Robicsek. 2009. Does my patient have Clostridium difficile infection? Ann. Intern. Med. 151:176-179.
26. Quinn, C. D., S. E. Sefers, W. Babiker, Y. He, R. Alcabasa, C. W. Stratton, K. C. Carroll, and Y. W. Tang. 2010. C. Diff Quik Chek Complete enzyme immunoassay provides a reliable first-line method for detection of Clostridium difficile in stool specimens. J. Clin. Microbiol. 48:603-605.
27. Reller, M. E., C. A. Lema, T. M. Perl, M. Cai, T. L. Ross, K. A. Speck, and K. C. Carroll. 2007. Yield of stool culture with isolate toxin testing versus a two-step algorithm including stool toxin testing for detection of toxigenic Clostridium difficile. J. Clin. Microbiol. 45:3601-3605.
28. Schmidt, M. L., and P. H. Gilligan. 2009. Clostridium difficile testing algorithms: what is practical and feasible? Anaerobe 15:270-273.
29. Sloan, L. M., B. J. Duresko, D. R. Gustafson, and J. E. Rosenblatt. 2008. Comparison of real-time PCR for detection of the tcdC gene with four toxin immunoassays and culture in diagnosis of Clostridium difficile infection. J. Clin. Microbiol. 46:1996-2001.
30. Stamper, P. D., R. Alcabasa, D. Aird, W. Babiker, J. Wehrlin, I. Ikpeama, and K. C. Carroll. 2009. Comparison of a commercial real-time PCR assay for tcdB detection to a cell culture cytotoxicity assay and toxigenic culture for direct detection of toxin-producing Clostridium difficile in clinical samples. J. Clin. Microbiol. 47:373-378.
31. Stamper, P. D., W. Babiker, R. Alcabasa, D. Aird, J. Wehrlin, I. Ikpeama, L. Gluck, and K. C. Carroll. 2009. Evaluation of a new commercial TaqMan PCR assay for direct detection of the Clostridium difficile toxin B gene in clinical stool specimens. J. Clin. Microbiol. 47:3846-3850.
32. Stubbs, S. L., J. S. Brazier, G. L. O'Neill, and B. I. Duerden. 1999. PCR targeted to the 16S-23S rRNA gene intergenic spacer region of Clostridium difficile and construction of a library consisting of 116 different PCR ribotypes. J. Clin. Microbiol. 37:461-463.
33. Sundram, F., A. Guyot, I. Carboo, S. Green, M. Lilaonitkul, and A. Scourfield. 2009. Clostridium difficile ribotypes 027 and 106: clinical outcomes and risk factors. J. Hosp. Infect. 72:111-118.
34. Svenungsson, B., L. G. Burman, K. Jalakas-Pornull, A. Lagergren, J. Struwe, and T. Akerlund. 2003. Epidemiology and molecular characterization of Clostridium difficile strains from patients with diarrhea: low disease incidence and evidence of limited cross-infection in a Swedish teaching hospital. J. Clin. Microbiol. 41:4031-4037.
35. Swindells, J., N. Brenwald, N. Reading, and B. Oppenheim. 2010. Evaluation of diagnostic tests for Clostridium difficile infection. J. Clin. Microbiol. 48:606-608.
36. Ticehurst, J. R., D. Z. Aird, L. M. Dam, A. P. Borek, J. T. Hargrove, and K. C. Carroll. 2006. Effective detection of toxigenic Clostridium difficile by a two-step algorithm including tests for antigen and cytotoxin. J. Clin. Microbiol. 44:1145-1149.