Diagnostic accuracy of a loop-mediated isothermal PCR assay for detection of Orientia tsutsugamushi during acute scrub typhus infection

PLoS Neglected Tropical Diseases

Volumn 5, Issue 9, 2011, Pages

  Paris, Daniel H ^a,b   Blacksell, Stuart D ^a,b   Nawtaisong, Pruksa ^a   Jenjaroen, Kemajittra ^a   Teeraratkul, Achara ^c   Chierakul, Wirongrong ^a   Wuthiekanun, Vanaporn ^a   Kantipong, Pacharee ^c   Day, Nicholas P J ^a,b  

^a MAHIDOL UNIVERSITY   (Thailand)

^b CHURCHILL HOSPITAL   (United Kingdom)

^c Chiang Rai Prachanukhao Hospital   (Thailand)

Author keywords

[No Author keywords available]

Indexed keywords

IMMUNOGLOBULIN M; BACTERIAL DNA; BACTERIUM ANTIBODY;

ADOLESCENT; ADULT; ARTICLE; BACTERIUM DETECTION; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DISEASE COURSE; ENDEMIC DISEASE; FEMALE; HUMAN; IMMUNOFLUORESCENCE TEST; LOOP MEDIATED ISOTHERMAL AMPLIFICATION; MAJOR CLINICAL STUDY; MALE; ORIENTIA TSUTSUGAMUSHI; OUTCOME ASSESSMENT; POLYMERASE CHAIN REACTION; PROSPECTIVE STUDY; SCRUB TYPHUS; SENSITIVITY AND SPECIFICITY; THAILAND; ACUTE DISEASE; ANIMAL; BLOOD; CERCOPITHECUS; CHEMISTRY; FEVER; FLUORESCENT ANTIBODY TECHNIQUE; GENETICS; IMMUNOLOGY; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; MIDDLE AGED; PREDICTIVE VALUE; REPRODUCIBILITY; VERO CELL;

ACUTE DISEASE; ADULT; ANIMALS; ANTIBODIES, BACTERIAL; CERCOPITHECUS AETHIOPS; DNA, BACTERIAL; FEMALE; FEVER; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; HUMANS; IMMUNOGLOBULIN M; MALE; MIDDLE AGED; ORIENTIA TSUTSUGAMUSHI; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE OF TESTS; PROSPECTIVE STUDIES; REPRODUCIBILITY OF RESULTS; SCRUB TYPHUS; THAILAND; VERO CELLS;

EID: 80053460822     PISSN: 19352727     EISSN: 19352735     Source Type: Journal    
DOI: 10.1371/journal.pntd.0001307     Document Type: Article

References (19)

1. Phongmany S, Rolain JM, Phetsouvanh R, Blacksell SD, Soukkhaseum V, et al. (2006) Rickettsial infections and fever, Vientiane, Laos. Emerg Infect Dis 12: 256-262.
2. Manosroi J, Chutipongvivate S, Auwanit W, Manosroi A, (2006) Determination and geographic distribution of Orientia tsutsugamushi serotypes in Thailand by nested polymerase chain reaction. Diagn Microbiol Infect Dis 55: 185-190.
3. Blacksell SD, Bryant NJ, Paris DH, Doust JA, Sakoda Y, et al. (2007) Scrub typhus serologic testing with the indirect immunofluorescence method as a diagnostic gold standard: a lack of consensus leads to a lot of confusion. Clin Infect Dis 44: 391-401.
4. Robinson DM, Brown G, Gan E, Huxsoll DL, (1976) Adaptation of a microimmunofluorescence test to the study of human Rickettsia tsutsugamushi antibody. Am J Trop Med Hyg 25: 900-905.
5. Koh GC, Maude RJ, Paris DH, Newton PN, Blacksell SD, (2010) Diagnosis of Scrub Typhus. Am J Trop Med Hyg 82: 368-370.
6. Paris DH, Aukkanit N, Jenjaroen K, Blacksell SD, Day NP, (2009) A highly sensitive quantitative real-time PCR assay based on the groEL gene of contemporary Thai strains of Orientia tsutsugamushi. Clin Microbiol Infect 15: 488-495.
7. Nagamine K, Hase T, Notomi T, (2002) Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol Cell Probes 16: 223-229.
8. Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, et al. (2000) Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28: E63.
9. Mori Y, Nagamine K, Tomita N, Notomi T, (2001) Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem Biophys Res Commun 289: 150-154.
10. Luksameetanasan R, Blacksell SD, Kalambaheti T, Wuthiekanun V, Chierakul W, et al. (2007) Patient and sample-related factors that effect the success of in vitro isolation of Orientia tsutsugamushi. Southeast Asian J Trop Med Public Health 38: 175-180.
11. Horinouchi H, Murai K, Okayama A, Nagatomo Y, Tachibana N, et al. (1996) Genotypic identification of Rickettsia tsutsugamushi by restriction fragment length polymorphism analysis of DNA amplified by the polymerase chain reaction. Am J Trop Med Hyg 54: 647-651.
12. Jiang J, Chan TC, Temenak JJ, Dasch GA, Ching WM, et al. (2004) Development of a quantitative real-time polymerase chain reaction assay specific for Orientia tsutsugamushi. Am J Trop Med Hyg 70: 351-356.
13. Paris DH, Blacksell SD, Stenos J, Graves SR, Unsworth NB, et al. (2008) Real-time multiplex PCR assay for detection and differentiation of rickettsiae and orientiae. Trans R Soc Trop Med Hyg 102: 186-193.
14. Paris DH, Blacksell SD, Newton PN, Day NP, (2008) Simple, rapid and sensitive detection of Orientia tsutsugamushi by loop-isothermal DNA amplification. Trans R Soc Trop Med Hyg 102: 1239-1246.
15. Brown GW, Shirai A, Rogers C, Groves MG, (1983) Diagnostic criteria for scrub typhus: probability values for immunofluorescent antibody and Proteus OXK agglutinin titers. Am J Trop Med Hyg 32: 1101-1107.
16. Coleman RE, Sangkasuwan V, Suwanabun N, Eamsila C, Mungviriya S, et al. (2002) Comparative evaluation of selected diagnostic assays for the detection of IgG and IgM antibody to Orientia tsutsugamushi in Thailand. Am J Trop Med Hyg 67: 497-503.
17. Kelly DJ, Fuerst PA, Ching WM, Richards AL, (2009) Scrub typhus: the geographic distribution of phenotypic and genotypic variants of Orientia tsutsugamushi. Clin Infect Dis 48 (Suppl 3): S203-S230.
18. Blacksell SD, Mammen MP, Thongpaseuth S, Gibbons RV, Jarman RG, et al. (2008) Evaluation of the PanBio dengue virus nonstructural 1 antigen detection and immunoglobulin M antibody enzyme-linked immunosorbent assays for the diagnosis of acute dengue infections in Laos. Diagn Microbiol Infect Dis 60: 43-49.
19. Dussart P, Petit L, Labeau B, Bremand L, Leduc A, et al. (2008) Evaluation of two new commercial tests for the diagnosis of acute dengue virus infection using NS1 antigen detection in human serum. PLoS Negl Trop Dis 2: e280.