메뉴 건너뛰기




Volumn 1073, Issue , 2013, Pages 31-42

Overlap extension PCR cloning

Author keywords

Overlap extension PCR cloning; Phusion; Recombinant vector; Restriction enzyme ligation independent

Indexed keywords

DNA POLYMERASE; POLYDEOXYRIBONUCLEOTIDE SYNTHASE;

EID: 84884198697     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-625-2_4     Document Type: Article
Times cited : (38)

References (25)
  • 2
    • 77954129380 scopus 로고    scopus 로고
    • One-step DNA fragment assembly and circularization for gene cloning
    • Zuo P, Rabie BM (2009) One-step DNA fragment assembly and circularization for gene cloning. Curr Issues Mol Biol 12:11-16
    • (2009) Curr Issues Mol Biol , vol.12 , pp. 11-16
    • Zuo, P.1    Rabie, B.M.2
  • 3
    • 0025321244 scopus 로고
    • PCR-induced (ligase-free) subcloning: A rapid reliable method to subclone polymerase chain reaction (PCR) products
    • Shuldiner AR, Scott LA, Roth J (1990) PCR-induced (ligase-free) subcloning: a rapid reliable method to subclone polymerase chain reaction (PCR) products. Nucleic Acids Res 18:1920
    • (1990) Nucleic Acids Res , vol.18 , pp. 1920
    • Shuldiner, A.R.1    Scott, L.A.2    Roth, J.3
  • 4
    • 0025904666 scopus 로고
    • Ligasefree subcloning: A versatile method to subclone polymerase chain reaction (PCR) products in a single day
    • Shuldiner AR, Tanner K et al (1991) Ligasefree subcloning: a versatile method to subclone polymerase chain reaction (PCR) products in a single day. Anal Biochem 194:9-15
    • (1991) Anal Biochem , vol.194 , pp. 9-15
    • Shuldiner, A.R.1    Tanner, K.2
  • 5
    • 68149136937 scopus 로고    scopus 로고
    • Circular polymerase extension cloning of complex gene libraries and pathways
    • Quan J, Tian J (2009) Circular polymerase extension cloning of complex gene libraries and pathways. PLoS One 4:e6441
    • (2009) PLoS One , vol.4
    • Quan, J.1    Tian, J.2
  • 6
    • 0034947940 scopus 로고    scopus 로고
    • Integration of PCR fragments at any specific site within cloning vectors without the use of restriction enzymes and DNA ligase
    • 92
    • Geiser M, Cebe R, Drewello D, Schmitz R (2001) Integration of PCR fragments at any specific site within cloning vectors without the use of restriction enzymes and DNA ligase. Biotechniques 31:88-90, 92
    • (2001) Biotechniques , vol.31 , pp. 88-90
    • Geiser, M.1    Cebe, R.2    Drewello, D.3    Schmitz, R.4
  • 7
    • 33645021327 scopus 로고    scopus 로고
    • RF cloning: A restriction-free method for inserting target genes into plasmids
    • van den Ent F, Lowe J (2006) RF cloning: a restriction-free method for inserting target genes into plasmids. J Biochem Biophys Methods 67:67-74
    • (2006) J Biochem Biophys Methods , vol.67 , pp. 67-74
    • Van Den Ent, F.1    Lowe, J.2
  • 8
    • 77956184308 scopus 로고    scopus 로고
    • Applications of the restriction free (RF) cloning procedure for molecular manipulations and protein expression
    • Unger T, Jacobovitch Y et al (2010) Applications of the Restriction Free (RF) cloning procedure for molecular manipulations and protein expression. J Struct Biol 172:34-44
    • (2010) J Struct Biol , vol.172 , pp. 34-44
    • Unger, T.1    Jacobovitch, Y.2
  • 9
    • 77954129578 scopus 로고    scopus 로고
    • Overlap extension PCR cloning: A simple and reliable way to create recombinant plasmids
    • Bryksin AV, Matsumura I (2010) Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids. Biotechniques 48:463-465
    • (2010) Biotechniques , vol.48 , pp. 463-465
    • Bryksin, A.V.1    Matsumura, I.2
  • 10
    • 84884171782 scopus 로고    scopus 로고
    • QuickChange, Stratagene
    • QuickChange http://www.stratagene.com/manuals/210513.pdf. Stratagene
  • 11
    • 80355134500 scopus 로고    scopus 로고
    • Transformation of plasmid DNA into E. Coli using the heat shock method
    • Froger A, Hall JE (2007) Transformation of plasmid DNA into E. coli using the heat shock method. J Vis Exp 2007:253
    • (2007) J Vis Exp , vol.2007 , pp. 253
    • Froger, A.1    Hall, J.E.2
  • 12
    • 67650699321 scopus 로고    scopus 로고
    • Plasmid uptake by bacteria: A comparison of methods and efficiencies
    • Yoshida N, Sato M (2009) Plasmid uptake by bacteria: a comparison of methods and efficiencies. Appl Microbiol Biotechnol 83:791-798
    • (2009) Appl Microbiol Biotechnol , vol.83 , pp. 791-798
    • Yoshida, N.1    Sato, M.2
  • 13
    • 78149431217 scopus 로고    scopus 로고
    • Construction of long DNA molecules from multiple fragments using PCR
    • In: Hughes S, Moody A (eds). Scion Publishing, London
    • Shevchuk NA, Bryksin AV (2007) Construction of long DNA molecules from multiple fragments using PCR. In: Hughes S, Moody A (eds) PCR (Methods Express Series). Scion Publishing, London, pp 197-216
    • (2007) PCR (Methods Express Series) , pp. 197-216
    • Shevchuk, N.A.1    Bryksin, A.V.2
  • 14
    • 79958279010 scopus 로고    scopus 로고
    • Generation of families of construct variants using golden gate shuffling
    • Engler C, Marillonnet S (2011) Generation of families of construct variants using golden gate shuffling. Methods Mol Biol 729:167-181
    • (2011) Methods Mol Biol , vol.729 , pp. 167-181
    • Engler, C.1    Marillonnet, S.2
  • 15
    • 67349270900 scopus 로고    scopus 로고
    • Enzymatic assembly of DNA molecules up to several hundred kilobases
    • Gibson DG, Young L et al (2009) Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat Methods 6:343-345
    • (2009) Nat Methods , vol.6 , pp. 343-345
    • Gibson, D.G.1    Young, L.2
  • 16
    • 59649108349 scopus 로고    scopus 로고
    • DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways
    • Shao Z, Zhao H (2009) DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways. Nucleic Acids Res 37: e16
    • (2009) Nucleic Acids Res , vol.37
    • Shao, Z.1    Zhao, H.2
  • 17
    • 0025675856 scopus 로고
    • High efficiency transformation of Escherichia coli with plasmids
    • Inoue H, Nojima H, Okayama H (1990) High efficiency transformation of Escherichia coli with plasmids. Gene 96:23-28
    • (1990) Gene , vol.96 , pp. 23-28
    • Inoue, H.1    Nojima, H.2    Okayama, H.3
  • 18
    • 17144450611 scopus 로고    scopus 로고
    • Construction of long DNA molecules using long PCR-based fusion of several fragments simultaneously
    • Shevchuk NA, Bryksin AV et al (2004) Construction of long DNA molecules using long PCR-based fusion of several fragments simultaneously. Nucleic Acids Res 32:e19
    • (2004) Nucleic Acids Res , vol.32
    • Shevchuk, N.A.1    Bryksin, A.V.2
  • 19
    • 84884172751 scopus 로고    scopus 로고
    • Restriction Free Cloning
    • Restriction Free Cloning (http://www.rf-cloning. org/)
  • 20
    • 84884183922 scopus 로고    scopus 로고
    • http://primer3.sourceforge.net
  • 21
    • 84884191068 scopus 로고    scopus 로고
    • http://www.bioinformatics.org/jambw/5/2/index.html
  • 22
    • 84884190176 scopus 로고    scopus 로고
    • http://www.ncbi.nlm.nih.gov/tools/primerblast
  • 23
    • 84884178400 scopus 로고    scopus 로고
    • http://www.idtdna.com/analyzer/Applications/OligoAnalyzer
  • 24
    • 0017572099 scopus 로고
    • Endo R DpnI restriction of Escherichia coli DNA synthesized in vitro. Evidence that the ends of Okazaki pieces are determined by template deoxynucleotide sequence
    • Gomez-Eichelmann MC, Lark KG (1977) Endo R DpnI restriction of Escherichia coli DNA synthesized in vitro. Evidence that the ends of Okazaki pieces are determined by template deoxynucleotide sequence. J Mol Biol 117:621-635
    • (1977) J Mol Biol , vol.117 , pp. 621-635
    • Gomez-Eichelmann, M.C.1    Lark, K.G.2
  • 25
    • 80053929147 scopus 로고    scopus 로고
    • Fast cloning: A highly simplified, purification-free, sequence-and ligation-independent PCR cloning method
    • Li C, Wen A et al (2011) Fast Cloning: a highly simplified, purification-free, sequence-and ligation-independent PCR cloning method. BMC Biotechnol 11:92
    • (2011) BMC Biotechnol , vol.11 , pp. 92
    • Li, C.1    Wen, A.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.