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Volumn 8, Issue 9, 2013, Pages 1080-1089

Kinetic isotope effects significantly influence intracellular metabolite 13C labeling patterns and flux determination

Author keywords

Isotope Effects; Isotopomer Modeling; Metabolic Engineering; Metabolic Flux Analysis; Modeling errors

Indexed keywords

CENTRAL CARBON METABOLISMS; INTRACELLULAR METABOLITES; ISOTOPE EFFECT; ISOTOPOMER MODELING; METABOLIC FLUX ANALYSIS; METABOLIC FLUX DISTRIBUTION; MODEL ERRORS; STATISTICAL SIGNIFICANCE;

EID: 84883803981     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201200276     Document Type: Article
Times cited : (24)

References (50)
  • 1
    • 0347506028 scopus 로고    scopus 로고
    • It is all about metabolic fluxes.
    • Nielsen, J., It is all about metabolic fluxes. J. Bacteriol. 2003, 185, 7031-7035.
    • (2003) J. Bacteriol. , vol.185 , pp. 7031-7035
    • Nielsen, J.1
  • 2
    • 33846061120 scopus 로고    scopus 로고
    • Metabolic networks in motion: 13C-based flux analysis.
    • Sauer, U., Metabolic networks in motion: 13C-based flux analysis. Mol. Syst. Biol. 2006, 2, 62.
    • (2006) Mol. Syst. Biol. , vol.2 , pp. 62
    • Sauer, U.1
  • 3
    • 0035140099 scopus 로고    scopus 로고
    • Network identification and flux quantification in the central metabolism of Saccharomyces cerevisiae under different conditions of glucose repression.
    • Gombert, A. K., dos Santos, M. M., Christensen, B., Nielsen, J., Network identification and flux quantification in the central metabolism of Saccharomyces cerevisiae under different conditions of glucose repression. J. Bacteriol. 2001, 183, 1441-1451.
    • (2001) J. Bacteriol. , vol.183 , pp. 1441-1451
    • Gombert, A.K.1    dos Santos, M.M.2    Christensen, B.3    Nielsen, J.4
  • 4
    • 20044375201 scopus 로고    scopus 로고
    • Large-scale in vivo flux analysis shows rigidity and suboptimal performance of Bacillus subtilis metabolism.
    • Fischer, E., Sauer, U., Large-scale in vivo flux analysis shows rigidity and suboptimal performance of Bacillus subtilis metabolism. Nat. Genet. 2005, 37, 636-640.
    • (2005) Nat. Genet. , vol.37 , pp. 636-640
    • Fischer, E.1    Sauer, U.2
  • 5
    • 0036956758 scopus 로고    scopus 로고
    • Genealogy profiling through strain improvement by using metabolic network analysis: Metabolic flux genealogy of several generations of lysine-producing corynebacteria.
    • Wittmann, C., Heinzle, E., Genealogy profiling through strain improvement by using metabolic network analysis: Metabolic flux genealogy of several generations of lysine-producing corynebacteria. Appl. Environ. Microbiol. 2002, 68, 5843-5859.
    • (2002) Appl. Environ. Microbiol. , vol.68 , pp. 5843-5859
    • Wittmann, C.1    Heinzle, E.2
  • 6
    • 34249297627 scopus 로고    scopus 로고
    • Metabolic flux analysis in a nonstationary system: Fed-batch fermentation of a high yielding strain of E. coli producing 1,3-propanediol.
    • Antoniewicz, M. R., Kraynie, D. F., Laffend, L. A., González-Lergier, J. et al., Metabolic flux analysis in a nonstationary system: Fed-batch fermentation of a high yielding strain of E. coli producing 1, 3-propanediol. Metab. Eng. 2007, 9, 277-292.
    • (2007) Metab. Eng. , vol.9 , pp. 277-292
    • Antoniewicz, M.R.1    Kraynie, D.F.2    Laffend, L.A.3    González-Lergier, J.4
  • 7
    • 42249096434 scopus 로고    scopus 로고
    • Metabolic flux analysis as a tool in metabolic engineering of plants.
    • Schwender, J., Metabolic flux analysis as a tool in metabolic engineering of plants. Curr. Opin. Biotechnol. 2008, 19, 131-137.
    • (2008) Curr. Opin. Biotechnol. , vol.19 , pp. 131-137
    • Schwender, J.1
  • 8
    • 68249110203 scopus 로고    scopus 로고
    • Metabolic flux analysis in plants: Coping with complexity.
    • Allen, D. K., Libourel, I. G. L., Shachar-Hill, Y., Metabolic flux analysis in plants: Coping with complexity. Plant Cell Environ. 2009, 32, 1241-1257.
    • (2009) Plant Cell Environ. , vol.32 , pp. 1241-1257
    • Allen, D.K.1    Libourel, I.G.L.2    Shachar-Hill, Y.3
  • 9
    • 84872270052 scopus 로고    scopus 로고
    • Expanding the concepts and tools of metabolic engineering to elucidate cancer metabolism.
    • Keibler, M. A., Fendt, S. M., Stephanopoulos, G., Expanding the concepts and tools of metabolic engineering to elucidate cancer metabolism. Biotechnol. Prog. 2012, 28, 1409-1418.
    • (2012) Biotechnol. Prog. , vol.28 , pp. 1409-1418
    • Keibler, M.A.1    Fendt, S.M.2    Stephanopoulos, G.3
  • 10
    • 53649110425 scopus 로고    scopus 로고
    • Systems-level metabolic flux profiling identifies fatty acid synthesis as a target for antiviral therapy.
    • Munger, J., Bennett, B. D., Parikh, A., Feng, X. J. et al., Systems-level metabolic flux profiling identifies fatty acid synthesis as a target for antiviral therapy. Nat. Biotechnol. 2008, 26, 1179-1186.
    • (2008) Nat. Biotechnol. , vol.26 , pp. 1179-1186
    • Munger, J.1    Bennett, B.D.2    Parikh, A.3    Feng, X.J.4
  • 11
    • 0032838693 scopus 로고    scopus 로고
    • Quantitative analysis of metabolic fluxes in Escherichia coli, using two-dimensional NMR spectroscopy and complete isotopomer models.
    • Schmidt, K., Nielsen, J., Villadsen, J., Quantitative analysis of metabolic fluxes in Escherichia coli, using two-dimensional NMR spectroscopy and complete isotopomer models. J. Biotechnol. 1999, 71, 175-189.
    • (1999) J. Biotechnol. , vol.71 , pp. 175-189
    • Schmidt, K.1    Nielsen, J.2    Villadsen, J.3
  • 12
    • 0027572102 scopus 로고
    • Metabolic flux distributions in Corynebacterium glutamicum during growth and lysine overproduction.
    • Vallino, J. J., Stephanopoulos, G., Metabolic flux distributions in Corynebacterium glutamicum during growth and lysine overproduction. Biotechnol. Bioeng. 1993, 41, 633-646.
    • (1993) Biotechnol. Bioeng. , vol.41 , pp. 633-646
    • Vallino, J.J.1    Stephanopoulos, G.2
  • 13
    • 0030050268 scopus 로고    scopus 로고
    • Determination of the fluxes in the central metabolism of Corynebacterium glutamicum by nuclear magnetic resonance spectroscopy combined with metabolite balancing.
    • Marx, A., de Graaf, A. A., Wiechert, W., Eggeling, L., Sahm, H., Determination of the fluxes in the central metabolism of Corynebacterium glutamicum by nuclear magnetic resonance spectroscopy combined with metabolite balancing. Biotechnol. Bioeng. 1996, 49, 111-129.
    • (1996) Biotechnol. Bioeng. , vol.49 , pp. 111-129
    • Marx, A.1    de Graaf, A.A.2    Wiechert, W.3    Eggeling, L.4    Sahm, H.5
  • 14
    • 0029146299 scopus 로고
    • Biosynthetically directed fractional 13C-labeling of proteinogenic amino acids. An efficient analytical tool to investigate intermediary metabolism.
    • Szyperski, T., Biosynthetically directed fractional 13C-labeling of proteinogenic amino acids. An efficient analytical tool to investigate intermediary metabolism. Eur. J. Biochem. 1995, 232, 433-448.
    • (1995) Eur. J. Biochem. , vol.232 , pp. 433-448
    • Szyperski, T.1
  • 15
    • 0031004921 scopus 로고    scopus 로고
    • Metabolic fluxes in riboflavin-producing Bacillus subtilis.
    • Sauer, U., Hatzimanikatis, V., Bailey, J. E., Hochuli, M. et al., Metabolic fluxes in riboflavin-producing Bacillus subtilis. Nat. Biotechnol. 1997, 15, 448-452.
    • (1997) Nat. Biotechnol. , vol.15 , pp. 448-452
    • Sauer, U.1    Hatzimanikatis, V.2    Bailey, J.E.3    Hochuli, M.4
  • 16
    • 0033205580 scopus 로고    scopus 로고
    • Isotopomer analysis using GC-MS.
    • Christensen, B., Nielsen, J., Isotopomer analysis using GC-MS. Metab. Eng. 1999, 1, 282-290.
    • (1999) Metab. Eng. , vol.1 , pp. 282-290
    • Christensen, B.1    Nielsen, J.2
  • 17
    • 0034233268 scopus 로고    scopus 로고
    • GC-MS analysis of amino acids rapidly provides rich information for isotopomer balancing.
    • Dauner, M., Sauer, U., GC-MS analysis of amino acids rapidly provides rich information for isotopomer balancing. Biotechnol. Prog. 2000, 16, 642-649.
    • (2000) Biotechnol. Prog. , vol.16 , pp. 642-649
    • Dauner, M.1    Sauer, U.2
  • 18
    • 0033586461 scopus 로고    scopus 로고
    • Mass spectrometry for metabolic flux analysis.
    • Wittmann, C., Heinzle, E., Mass spectrometry for metabolic flux analysis. Biotechnol. Bioeng. 1999, 62, 739-750.
    • (1999) Biotechnol. Bioeng. , vol.62 , pp. 739-750
    • Wittmann, C.1    Heinzle, E.2
  • 19
    • 0036271587 scopus 로고    scopus 로고
    • Analysis of flux estimates based on (13)C-labelling experiments.
    • Christensen, B., Gombert, A. K., Nielsen, J., Analysis of flux estimates based on (13)C-labelling experiments. Eur. J. Biochem. 2002, 269, 2795-2800.
    • (2002) Eur. J. Biochem. , vol.269 , pp. 2795-2800
    • Christensen, B.1    Gombert, A.K.2    Nielsen, J.3
  • 20
    • 0031554635 scopus 로고    scopus 로고
    • Bidirectional reaction steps in metabolic networks: I. Modeling and simulation of carbon isotope labeling experiments.
    • Wiechert, W., de Graaf, A. A., Bidirectional reaction steps in metabolic networks: I. Modeling and simulation of carbon isotope labeling experiments. Biotechnol. Bioeng. 1997, 55, 101-117.
    • (1997) Biotechnol. Bioeng. , vol.55 , pp. 101-117
    • Wiechert, W.1    de Graaf, A.A.2
  • 21
    • 0031554628 scopus 로고    scopus 로고
    • Bidirectional reaction steps in metabolic networks: II. Flux estimation and statistical analysis.
    • Wiechert, W., Siefke, C., de Graaf, A. A., Marx, A., Bidirectional reaction steps in metabolic networks: II. Flux estimation and statistical analysis. Biotechnol. Bioeng. 1997, 55, 118-135.
    • (1997) Biotechnol. Bioeng. , vol.55 , pp. 118-135
    • Wiechert, W.1    Siefke, C.2    de Graaf, A.A.3    Marx, A.4
  • 22
    • 0343267841 scopus 로고    scopus 로고
    • Bidirectional reaction steps in metabolic networks: III. Explicit solution and analysis of isotopomer labeling systems.
    • Wiechert, W., Möllney, M., Isermann, N., Wurzel, M., de Graaf, A. A., Bidirectional reaction steps in metabolic networks: III. Explicit solution and analysis of isotopomer labeling systems. Biotechnol. Bioeng. 1999, 66, 69-85.
    • (1999) Biotechnol. Bioeng. , vol.66 , pp. 69-85
    • Wiechert, W.1    Möllney, M.2    Isermann, N.3    Wurzel, M.4    de Graaf, A.A.5
  • 23
    • 4243829380 scopus 로고    scopus 로고
    • Bidirectional reaction steps in metabolic networks: IV. Optimal design of isotopomer labeling experiments.
    • Möllney, M., Wiechert, W., Kownatzki, D., de Graaf, A. A., Bidirectional reaction steps in metabolic networks: IV. Optimal design of isotopomer labeling experiments. Biotechnol. Bioeng. 1999, 66, 86-103.
    • (1999) Biotechnol. Bioeng. , vol.66 , pp. 86-103
    • Möllney, M.1    Wiechert, W.2    Kownatzki, D.3    de Graaf, A.A.4
  • 24
    • 0033107837 scopus 로고    scopus 로고
    • Quantification of intracellular metabolic fluxes from fractional enrichment and 13C-13C coupling constraints on the isotopomer distribution in labeled biomass components.
    • Schmidt, K., Nrregaard, L. C., Pedersen, B., Meissner, A. J. et al., Quantification of intracellular metabolic fluxes from fractional enrichment and 13C-13C coupling constraints on the isotopomer distribution in labeled biomass components. Metab. Eng. 1999, 1, 166-179.
    • (1999) Metab. Eng. , vol.1 , pp. 166-179
    • Schmidt, K.1    Nrregaard, L.C.2    Pedersen, B.3    Meissner, A.J.4
  • 25
    • 33845679072 scopus 로고    scopus 로고
    • Elementary metabolite units (EMU): A novel framework for modeling isotopic distributions.
    • Antoniewicz, M. R., Kelleher, J. K., Stephanopoulos, G., Elementary metabolite units (EMU): A novel framework for modeling isotopic distributions. Metab. Eng. 2007, 9, 68-86.
    • (2007) Metab. Eng. , vol.9 , pp. 68-86
    • Antoniewicz, M.R.1    Kelleher, J.K.2    Stephanopoulos, G.3
  • 26
    • 0014938598 scopus 로고
    • Carbon isotope discrimination in a plant possessing the C4 dicarboxylic acid pathway.
    • Whelan, T., Sackett, W. M., Benedict, C. R., Carbon isotope discrimination in a plant possessing the C4 dicarboxylic acid pathway. Biochem. Biophys. Res. Commun. 1970, 41, 1205-1210.
    • (1970) Biochem. Biophys. Res. Commun. , vol.41 , pp. 1205-1210
    • Whelan, T.1    Sackett, W.M.2    Benedict, C.R.3
  • 27
    • 0000983468 scopus 로고
    • Enzymatic fractionation of carbon isotopes by phosphoenolpyruvate carboxylase from c(4) plants.
    • Whelan, T., Sackett, W. M., Benedict, C. R., Enzymatic fractionation of carbon isotopes by phosphoenolpyruvate carboxylase from c(4) plants. Plant Physiol. 1973, 51, 1051-1054.
    • (1973) Plant Physiol. , vol.51 , pp. 1051-1054
    • Whelan, T.1    Sackett, W.M.2    Benedict, C.R.3
  • 28
    • 47749087002 scopus 로고    scopus 로고
    • Analysis of 13C labeling enrichment in microbial culture applying metabolic tracer experiments using gas chromatography-combustion-isotope ratio mass spectrometry.
    • Heinzle, E., Yuan, Y., Kumar, S., Wittmann, C. et al., Analysis of 13C labeling enrichment in microbial culture applying metabolic tracer experiments using gas chromatography-combustion-isotope ratio mass spectrometry. Anal. Biochem. 2008, 380, 202-210.
    • (2008) Anal. Biochem. , vol.380 , pp. 202-210
    • Heinzle, E.1    Yuan, Y.2    Kumar, S.3    Wittmann, C.4
  • 29
    • 0034813964 scopus 로고    scopus 로고
    • Possible pitfalls of flux calculations based on (13)C-labeling.
    • van Winden, W., Verheijen, P., Heijnen, S., Possible pitfalls of flux calculations based on (13)C-labeling. Metab. Eng. 2001, 3, 151-162.
    • (2001) Metab. Eng. , vol.3 , pp. 151-162
    • van Winden, W.1    Verheijen, P.2    Heijnen, S.3
  • 30
    • 77953023574 scopus 로고    scopus 로고
    • 13C metabolic flux analysis for larger scale cultivation using gas chromatography-combustion-isotope ratio mass spectrometry.
    • Yuan, Y., Yang, T. H., Heinzle, E., 13C metabolic flux analysis for larger scale cultivation using gas chromatography-combustion-isotope ratio mass spectrometry. Metab. Eng. 2010, 12, 392-400.
    • (2010) Metab. Eng. , vol.12 , pp. 392-400
    • Yuan, Y.1    Yang, T.H.2    Heinzle, E.3
  • 31
    • 0001544961 scopus 로고
    • Carbon isotope fractionation in formation of amino acids by photosynthetic organisms.
    • Abelson, P. H., Hoering, T. C., Carbon isotope fractionation in formation of amino acids by photosynthetic organisms. Proc. Natl. Acad. Sci. USA 1961, 47, 623-632.
    • (1961) Proc. Natl. Acad. Sci. USA , vol.47 , pp. 623-632
    • Abelson, P.H.1    Hoering, T.C.2
  • 32
    • 0001434373 scopus 로고
    • Metabolic fractionation of C13 & C12 in plants.
    • Park, R., Epstein, S., Metabolic fractionation of C13 & C12 in plants. Plant Physiol. 1961, 36, 133-138.
    • (1961) Plant Physiol. , vol.36 , pp. 133-138
    • Park, R.1    Epstein, S.2
  • 33
    • 0019897657 scopus 로고
    • Carbon isotopic fractionation in the biosynthesis of bacterial fatty acids. Ozonolysis of unsaturated fatty acids as a means of determining the intramolecular distribution of carbon isotopes.
    • Monson, K. D., Hayes, J. M., Carbon isotopic fractionation in the biosynthesis of bacterial fatty acids. Ozonolysis of unsaturated fatty acids as a means of determining the intramolecular distribution of carbon isotopes. Geochim. Cosmochim. Acta 1982, 46, 139-149.
    • (1982) Geochim. Cosmochim. Acta , vol.46 , pp. 139-149
    • Monson, K.D.1    Hayes, J.M.2
  • 34
    • 0023655334 scopus 로고
    • Carbon isotope effects on the pyruvate dehydrogenase reaction and their importance for relative carbon-13 depletion in lipids.
    • Melzer, E., Schmidt, H. L., Carbon isotope effects on the pyruvate dehydrogenase reaction and their importance for relative carbon-13 depletion in lipids. J. Biol. Chem. 1987, 262, 8159-8164.
    • (1987) J. Biol. Chem. , vol.262 , pp. 8159-8164
    • Melzer, E.1    Schmidt, H.L.2
  • 35
    • 0346786497 scopus 로고    scopus 로고
    • Fundamentals and systematics of the non-statistical distributions of isotopes in natural compounds.
    • Schmidt, H. L., Fundamentals and systematics of the non-statistical distributions of isotopes in natural compounds. Naturwissenschaften 2003, 90, 537-552.
    • (2003) Naturwissenschaften , vol.90 , pp. 537-552
    • Schmidt, H.L.1
  • 36
    • 0017374594 scopus 로고
    • Mechanism of carbon isotope fractionation associated with lipid synthesis.
    • DeNiro, M. J., Epstein, S., Mechanism of carbon isotope fractionation associated with lipid synthesis. Science 1977, 197, 261-263.
    • (1977) Science , vol.197 , pp. 261-263
    • DeNiro, M.J.1    Epstein, S.2
  • 37
    • 0037335931 scopus 로고    scopus 로고
    • Metabolic flux profiling of Escherichia coli mutants in central carbon metabolism using GC-MS.
    • Fischer, E., Sauer, U., Metabolic flux profiling of Escherichia coli mutants in central carbon metabolism using GC-MS. Eur. J. Biochem. 2003, 270, 880-891.
    • (2003) Eur. J. Biochem. , vol.270 , pp. 880-891
    • Fischer, E.1    Sauer, U.2
  • 38
    • 25444467580 scopus 로고    scopus 로고
    • Large-scale 13C-flux analysis reveals mechanistic principles of metabolic network robustness to null mutations in yeast.
    • Blank, L. M., Kuepfer, L., Sauer, U., Large-scale 13C-flux analysis reveals mechanistic principles of metabolic network robustness to null mutations in yeast. Genome Biol. 2005, 6, R49.
    • (2005) Genome Biol. , vol.6
    • Blank, L.M.1    Kuepfer, L.2    Sauer, U.3
  • 39
    • 77949447610 scopus 로고    scopus 로고
    • Transcriptional regulation of respiration in yeast metabolizing differently repressive carbon substrates.
    • Fendt, S. M., Sauer, U., Transcriptional regulation of respiration in yeast metabolizing differently repressive carbon substrates. BMC Syst. Biol. 2010, 4, 12.
    • (2010) BMC Syst. Biol. , vol.4 , pp. 12
    • Fendt, S.M.1    Sauer, U.2
  • 40
    • 70449519259 scopus 로고    scopus 로고
    • Evaluation of 13C isotopic tracers for metabolic flux analysis in mammalian cells.
    • Metallo, C. M., Walther, J. L., Stephanopoulos, G., Evaluation of 13C isotopic tracers for metabolic flux analysis in mammalian cells. J. Biotechnol. 2009, 144, 167-174.
    • (2009) J. Biotechnol. , vol.144 , pp. 167-174
    • Metallo, C.M.1    Walther, J.L.2    Stephanopoulos, G.3
  • 41
    • 84861008392 scopus 로고    scopus 로고
    • 13C-labeling experiments for metabolic flux analysis in mammalian cells.
    • 13C-labeling experiments for metabolic flux analysis in mammalian cells. BMC Syst. Biol. 2012, 6, 43.
    • (2012) BMC Syst. Biol. , vol.6 , pp. 43
    • Crown, S.B.1    Ahn, W.S.2    Antoniewicz, M.R.3
  • 42
    • 35348941242 scopus 로고    scopus 로고
    • Accurate assessment of amino acid mass isotopomer distributions for metabolic flux analysis.
    • Antoniewicz, M. R., Kelleher, J. K., Stephanopoulos, G., Accurate assessment of amino acid mass isotopomer distributions for metabolic flux analysis. Anal. Chem. 2007, 79, 7554-7559.
    • (2007) Anal. Chem. , vol.79 , pp. 7554-7559
    • Antoniewicz, M.R.1    Kelleher, J.K.2    Stephanopoulos, G.3
  • 43
    • 0031055060 scopus 로고    scopus 로고
    • Carbon isotope effects on the fructose-1,6-bisphosphate aldolase reaction, origin for non-statistical 13C distributions in carbohydrates.
    • Gleixner, G., Schmidt, H. L., Carbon isotope effects on the fructose-1, 6-bisphosphate aldolase reaction, origin for non-statistical 13C distributions in carbohydrates. J. Biol. Chem. 1997, 272, 5382-5387.
    • (1997) J. Biol. Chem. , vol.272 , pp. 5382-5387
    • Gleixner, G.1    Schmidt, H.L.2
  • 44
    • 0020492976 scopus 로고
    • Use of multiple isotope effects to determine enzyme mechanisms and intrinsic isotope effects. Malic enzyme and glucose-6-phosphate dehydrogenase.
    • Hermes, J. D., Roeske, C. A., O'Leary, M. H., Cleland, W. W., Use of multiple isotope effects to determine enzyme mechanisms and intrinsic isotope effects. Malic enzyme and glucose-6-phosphate dehydrogenase. Biochemistry 1982, 21, 5106-5114.
    • (1982) Biochemistry , vol.21 , pp. 5106-5114
    • Hermes, J.D.1    Roeske, C.A.2    O'Leary, M.H.3    Cleland, W.W.4
  • 45
    • 0021755240 scopus 로고
    • Use of multiple isotope effects to study the mechanism of 6-phosphogluconate dehydrogenase.
    • Rendina, A. R., Hermes, J. D., Cleland, W. W., Use of multiple isotope effects to study the mechanism of 6-phosphogluconate dehydrogenase. Biochemistry 1984, 23, 6257-6262.
    • (1984) Biochemistry , vol.23 , pp. 6257-6262
    • Rendina, A.R.1    Hermes, J.D.2    Cleland, W.W.3
  • 46
    • 0032506052 scopus 로고    scopus 로고
    • Multiple isotope effects as a probe of proton and hydride transfer in the 6-phosphogluconate dehydrogenase reaction.
    • Hwang, C. C., Cook, P. F., Multiple isotope effects as a probe of proton and hydride transfer in the 6-phosphogluconate dehydrogenase reaction. Biochemistry 1998, 37, 15698-15702.
    • (1998) Biochemistry , vol.37 , pp. 15698-15702
    • Hwang, C.C.1    Cook, P.F.2
  • 47
    • 0032497367 scopus 로고    scopus 로고
    • Oxidative decarboxylation of 6-phosphogluconate by 6-phosphogluconate dehydrogenase proceeds by a stepwise mechanism with NADP and APADP as oxidants.
    • Hwang, C. C., Berdis, A. J., Karsten, W. E., Cleland, W. W., Cook, P. F., Oxidative decarboxylation of 6-phosphogluconate by 6-phosphogluconate dehydrogenase proceeds by a stepwise mechanism with NADP and APADP as oxidants. Biochemistry 1998, 37, 12596-12602.
    • (1998) Biochemistry , vol.37 , pp. 12596-12602
    • Hwang, C.C.1    Berdis, A.J.2    Karsten, W.E.3    Cleland, W.W.4    Cook, P.F.5
  • 48
    • 0024291638 scopus 로고
    • Isotope effect studies of the chemical mechanism of pig heart NADP isocitrate dehydrogenase.
    • Grissom, C. B., Cleland, W. W., Isotope effect studies of the chemical mechanism of pig heart NADP isocitrate dehydrogenase. Biochemistry 1988, 27, 2934-2943.
    • (1988) Biochemistry , vol.27 , pp. 2934-2943
    • Grissom, C.B.1    Cleland, W.W.2
  • 49
    • 33745155105 scopus 로고    scopus 로고
    • Determination of confidence intervals of metabolic fluxes estimated from stable isotope measurements.
    • Antoniewicz, M. R., Kelleher, J. K., Stephanopoulos, G., Determination of confidence intervals of metabolic fluxes estimated from stable isotope measurements. Metab. Eng. 2006, 8, 324-337.
    • (2006) Metab. Eng. , vol.8 , pp. 324-337
    • Antoniewicz, M.R.1    Kelleher, J.K.2    Stephanopoulos, G.3
  • 50
    • 15044347498 scopus 로고    scopus 로고
    • Metabolic-flux analysis of Saccharomyces cerevisiae CEN.PK113-7D based on mass isotopomer measurements of (13)C-labeled primary metabolites.
    • van Winden, W. A., van Dam, J. C., Ras, C., Kleijn, R. J. et al., Metabolic-flux analysis of Saccharomyces cerevisiae CEN.PK113-7D based on mass isotopomer measurements of (13)C-labeled primary metabolites. FEMS Yeast Res. 2005, 5, 559-568.
    • (2005) FEMS Yeast Res. , vol.5 , pp. 559-568
    • van Winden, W.A.1    van Dam, J.C.2    Ras, C.3    Kleijn, R.J.4


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