Combination of multistep IMAC enrichment with high-pH reverse phase separation for in-depth phosphoproteomic profiling

Journal of Proteome Research

Volumn 12, Issue 9, 2013, Pages 4176-4186

  Yue, Xiao Shan ^a   Hummon, Amanda B ^a  

^a UNIVERSITY OF NOTRE DAME   (United States)

Author keywords

high p I phosphopeptides; high pH reverse phase fractionation; multistep IMAC enrichment; phosphoproteomics; SCX fractionation

Indexed keywords

PHOSPHOPEPTIDE;

ARTICLE; CELL FRACTIONATION; CELL LYSATE; HUMAN; HUMAN CELL; HYDROPHILICITY; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; LIPOPHILICITY; PHASE SEPARATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; WHOLE CELL;

CELL FRACTIONATION; CELL LINE; CHROMATOGRAPHY, ION EXCHANGE; CHROMATOGRAPHY, REVERSE-PHASE; HUMANS; HYDROGEN-ION CONCENTRATION; PHOSPHOPROTEINS; PROTEOME; PROTEOMICS; TANDEM MASS SPECTROMETRY;

EID: 84883780981     PISSN: 15353893     EISSN: 15353907     Source Type: Journal    
DOI: 10.1021/pr4005234     Document Type: Article

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