Multidimensional strategy for sensitive phosphoproteomics incorporating protein prefractionation combined with SIMAC, HILIC, and TiO 2 chromatography applied to proximal EGF signaling

Journal of Proteome Research

Volumn 10, Issue 12, 2011, Pages 5383-5397

  Engholm Keller, Kasper ^a   Hansen, Thomas Aarup ^a   Palmisano, Giuseppe ^a   Larsen, Martin R ^a  

^a UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

Author keywords

EGF signalling; false discovery rate estimation; multidimensional phosphopeptide fractionation; Phosphoproteomics

Indexed keywords

CELL PROTEIN; EPIDERMAL GROWTH FACTOR; PHOSPHOPEPTIDE; PHOSPHOPROTEIN; TITANIUM DIOXIDE;

ACCURACY; ARTICLE; CHROMATOGRAPHY; DATA ANALYSIS SOFTWARE; FALSE POSITIVE RESULT; FRACTIONATION; HUMAN; HYDROPHILIC INTERACTION CHROMATOGRAPHY; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; PHOSPHOPROTEOMICS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEOMICS; SEQUENTIAL ELUTION FROM IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; SIGNAL TRANSDUCTION;

ANIMALS; AUTOMATIC DATA PROCESSING; BINDING SITES; CARBONATES; CHEMICAL FRACTIONATION; CHROMATOGRAPHY, LIQUID; EPIDERMAL GROWTH FACTOR; HELA CELLS; HUMANS; PHOSPHOPEPTIDES; PHOSPHORYLATION; PROTEOMICS; REPRODUCIBILITY OF RESULTS; SIGNAL TRANSDUCTION; SOFTWARE; TIME FACTORS; ULTRACENTRIFUGATION;

EID: 82755163993     PISSN: 15353893     EISSN: 15353907     Source Type: Journal    
DOI: 10.1021/pr200641x     Document Type: Article

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