Multilocus phylogeography of the common lizard Zootoca vivipara at the Ibero-Pyrenean suture zone reveals lowland barriers and high-elevation introgression

BMC Evolutionary Biology

Volumn 13, Issue 1, 2013, Pages

  Milá, Borja ^a   Surget Groba, Yann ^b   Heulin, Benoît ^c   Gosá, Alberto ^d   Fitze, Patrick S ^a,e,f,g  

^a MUSEO NACIONAL DE CIENCIAS NATURALES   (Spain)

^b KEY LABORATORY OF TROPICAL FOREST ECOLOGY   (China)

^c UNIVERSITÉ DE RENNES 1   (France)

^d Herpetology Department   (Spain)

^e FUNDACIÓN ARAID   (Spain)

^f INSTITUTO PIRENAICO DE ECOLOGÍA   (Spain)

^g UNIVERSITY OF LAUSANNE   (Switzerland)

Author keywords

Cytonuclear incongruence; Gene flow; Phylogeography; Secondary contact; Speciation; Vicariance

Indexed keywords

COLONIZATION; DIVERGENCE; ELEVATION; GENE FLOW; GEOGRAPHICAL DISTRIBUTION; INTROGRESSION; LIZARD; MITOCHONDRIAL DNA; PHYLOGENY; PHYLOGEOGRAPHY; PLEISTOCENE; SPECIATION (BIOLOGY); SUTURE ZONE; VICARIANCE;

BASQUE COUNTRY; CANTABRIAN MOUNTAINS; FRANCE; IBERIAN PENINSULA; PYRENEES; SPAIN;

INVERTEBRATA; SQUAMATA; VERTEBRATA; ZOOTOCA VIVIPARA;

MITOCHONDRIAL DNA;

AMPLIFIED FRAGMENT LENGTH POLYMORPHISM; ANIMAL; ARTICLE; CLASSIFICATION; FRANCE; GENE FLOW; GENETICS; LIZARD; MOLECULAR GENETICS; PHYLOGEOGRAPHY; POPULATION GENETICS; SPAIN;

AMPLIFIED FRAGMENT LENGTH POLYMORPHISM ANALYSIS; ANIMALS; DNA, MITOCHONDRIAL; FRANCE; GENE FLOW; GENETICS, POPULATION; LIZARDS; MOLECULAR SEQUENCE DATA; PHYLOGEOGRAPHY; SPAIN;

EID: 84883701805     PISSN: None     EISSN: 14712148     Source Type: Journal    
DOI: 10.1186/1471-2148-13-192     Document Type: Article

References (89)

1. Avise JC, Phylogeography: the history and formation of species Cambridge, Massachusetts: Harvard University Press 2000
2. Analysis of hybrid zones. Barton N, Hewitt G, Ann Rev Ecol Syst 1985 16 113 148 10.1146/annurev.es.16.110185.000553
3. Genetic consequeneces of climatic oscillations in the Quaternary. Hewitt GM, Phil Trans R Soc Lond B 2004 359 183 195 10.1098/rstb.2003.1388 (Pubitemid 38231471)
4. The genetic legacy of the Quaternary ice ages. Hewitt GM, Nature (London) 2000 405 907 913 10.1038/35016000 (Pubitemid 30435039)
5. Comparative phylogeography and postglacial colonization routes in Europe. Taberlet P, Fumagalli L, Wust-Saucy AG, Cosson JF, Mol Ecol 1998 7 453 464 10.1046/j.1365-294x.1998.00289.x 9628000 (Pubitemid 28226692)
6. Phylogeography of western Palaearctic reptiles-Spatial and temporal speciation patterns. Joger U, Fritz U, Guicking D, Kalyabina-Hauf S, Nagy ZT, Wink M, Zool Anz 2007 246 293 313 10.1016/j.jcz.2007.09.002 (Pubitemid 350019261)
7. Refugia within refugia: patterns of phylogeographic concordance in the Iberian Peninsula. Gómez A, Lunt DH, Phylogeography of Southern European Refugia Netherlands: Springer, Weiss S, Ferrand N, 2007 155 188
8. Intraspecific phylogeography of Lacerta vivipara and the evolution of viviparity. Surget-Groba Y, Heulin B, Guillaume C-P, Thorpe RS, Kupriyanova L, Vogrin N, Maslak R, Mazzotti S, Venczel M, Ghira I, et al. Mol Phylogenet Evol 2001 18 449 459 10.1006/mpev.2000.0896 11277636 (Pubitemid 34299594)
9. Comparisons of mitochondrial DNA (mtDNA) sequences (16S rRNA gene) between oviparous and viviparous strains of Lacerta vivipara: a preliminary study. Heulin B, Surget-Groba Y, Guiller A, Guillaume C-P, Deunff J, Mol Ecol 1999 8 10 1627 1631 10.1046/j.1365-294x.1999.00746.x 10583826 (Pubitemid 29514703)
10. Refuge areas and suture zones in the Pyrenean and Cantabrian regions: geographic variation of the female MPI sex-linked alleles among oviparous populations of the lizard Lacerta (Zootoca) vivipara. Guillaume C-P, Heulin B, Arrayago MJ, Bea A, Braña F, Ecography 2000 23 3 10 10.1111/j.1600-0587. 2000.tb00255.x (Pubitemid 30167947)
11. Perspective: gene divergence, population divergence, and the variance in coalescence time in phylogeographic studies. Edwards SV, Beerli P, Evolution 2000 54 1839 1854 11209764 (Pubitemid 32113816)
12. Mitochondrial DNA under siege in avian phylogeography. Zink RM, Barrowclough GF, Mol Ecol 2008 17 2107 2121 10.1111/j.1365-294X.2008.03737.x 18397219 (Pubitemid 351562803)
13. Inferring phylogenies from mtDNA variation: Mitochondrial-gene trees versus nuclear-gene trees. Moore WS, Evolution 1995 49 718 726 10.2307/2410325
14. Genetic consequences of range expansions. Excoffier L, Foll M, Petit RJ, Ann Rev Ecol Syst 2009 40 481 501 10.1146/annurev.ecolsys.39.110707.173414
15. A cryptic contact zone between divergent mitochondrial DNA lineages in southwestern North America supports past introgressive hybridization in the yellow-rumped warbler complex (Aves: Dendroica coronata). Milá B, Toews DPL, Smith TB, Wayne RK, Biol J Linn Soc 2011 103 696 706 10.1111/j.1095-8312. 2011.01661.x
16. Mitochondrial introgression and incomplete lineage sorting through space and time: phylogenetics of crotaphytid lizards. McGuire JA, Linkem CW, Koo MS, Hutchison DW, Lappin AK, Orange DI, Lemos-Espinal J, Riddle BR, Jaeger JR, Crandall K, Evolution 2007 61 2879 2897 10.1111/j.1558-5646.2007.00239.x 17941840 (Pubitemid 350179375)
17. Species-level paraphyly and polyphyly: frequency, causes, and consequences, with insights from animal mitochondrial DNA. Funk DJ, Omland KE, Ann Rev Ecol Syst 2003 34 397 423 10.1146/annurev.ecolsys.34.011802.132421 (Pubitemid 38171425)
18. Population size does not influence mitochondrial genetic diversity in animals. Bazin E, Glémin S, Galtier N, Science 2006 28 570 572
19. Evolutionary implications of non-neutral mitochondrial genetic variation. Dowling DK, Friberg U, Lindell J, Trends Ecol Evol 2008 23 546 554 10.1016/j.tree.2008.05.011 18722688
20. The incomplete natural history of mitochondria. Ballard JWO, Whitlock MC, Mol Ecol 2004 13 729 744 10.1046/j.1365-294X.2003.02063.x 15012752 (Pubitemid 38390584)
21. Statistical phylogeography. Knowles LL, Ann Rev Ecol Syst 2009 40 593 612 10.1146/annurev.ecolsys.38.091206.095702
22. Pairwise comparisons of mitochondrial DNA sequences in stable and exponentially growing populations. Slatkin M, Hudson RR, Genetics 1991 129 555 562 1743491
23. Pritchard JK, Wen W, Documentation for Structure Software: Version 2 2004 Available at: http://pritch.bsd.uchicago.edu
24. Dynamics of haplogroup frequencies and survival rates in a contact zone of two mtDNA lineages of the lizard Lacerta vivipara. Heulin B, Surget-Groba Y, Sinervo B, Miles D, Guiller A, Ecography 2011 34 436 447 10.1111/j.1600-0587. 2010.06540.x
25. Postglacial expansion and genome subdivision in the European grasshopper Chorthippus parallelus. Cooper SJ, Ibrahim KM, Hewitt G, Mol Ecol 1995 4 49 60 10.1111/j.1365-294X.1995.tb00191.x 7711954
26. Male-biased gene flow across an avian hybrid zone: evidence from mitochondrial and microsatellite DNA. Helbig AJ, Salomon M, Bensch S, Seibold I, J Evol Biol 2001 14 277 287 10.1046/j.1420-9101.2001.00273.x (Pubitemid 32333200)
27. Conflicting phylogenetic signal of nuclear vs mitochondrial DNA markers in midwife toads (Anura, Discoglossidae, Alytes): deep coalescence or ancestral hybridization? Gonçalves H, Martínez-Solano I, Ferrand N, García-París M, Mol Phylogenet Evol 2007 44 494 500 10.1016/j.ympev.2007.03.001 17433723 (Pubitemid 46829925)
28. A new perspective on the evolutionary history of western European Sorex araneus group revealed by paternal and maternal molecular markers. Yannic G, Basset P, Hausser J, Mol Phylogenet Evol 2008 47 237 250 10.1016/j.ympev.2008. 01.029 18325791
29. Mitochondrial DNA phylogeography of European hedgehogs. Santucci F, Emerson BC, Hewitt G, Mol Ecol 1998 7 1163 1172 10.1046/j.1365-294x.1998.00436.x 9734073 (Pubitemid 28428491)
30. Mitochondrial DNA polymorphism, phylogeography, and conservation genetics of the brown bear Ursus arctos in Europe. Taberlet P, Bouvet J, Proc R Soc Lond B Biol Sci 1994 B 255 195 200
31. Lacerta vivipara Jaquin, 1797. Pérez-Mellado V, Fauna Ibérica, vol 10 Madrid: Museo Nacional de Ciencias Naturales, CSIC, Ramos MA, 1997 232 242 22963986
32. Le lézard vivipare. Heulin B, Guillaume C-P, Les reptiles de la France, Belgique, Luxembourg et Suisse Paris: Biotope, Publications du Museum National d'Histoire Naturelle, Vacher JP, Geniez M, 2010 394 401
33. Speciation in mountains: phylogeography and phylogeny of the rock lizards genus Iberolacerta (Reptilia: Lacertidae). Crochet P-A, Chaline O, Surget-Groba Y, Debain C, Cheylan M, Mol Phylogenet Evol 2004 30 860 866 10.1016/j.ympev.2003.07.016 15012966 (Pubitemid 38260983)
34. The legacy of ice ages in mountain species: post-glacial colonization of mountain tops rather than current range fragmentation determines mitochondrial genetic diversity in an endemic Pyrenean rock lizard. Mouret V, Guillaumet A, Cheylan M, Pottier G, Ferchaud AL, Crochet PA, J Biogeogr 2011 38 1717 1731 10.1111/j.1365-2699.2011.02514.x
35. The persistence of Pliocene populations through the Pleistocene climatic cycles: evidence from the phylogeography of an Iberian lizard. Paulo OS, Dias C, Bruford MW, Jordan WC, Nichols RA, Proc R Soc Lond B 2001 268 1625 1630 10.1098/rspb.2001.1706 (Pubitemid 32730419)
36. Contrasting patterns of population subdivision and historical demography in three western Mediterranean lizard species inferred from mitochondrial DNA variation. Pinho C, Harris DJ, Ferrand N, Mol Ecol 2007 16 1191 1205 10.1111/j.1365-294X.2007.03230.x 17391406 (Pubitemid 46354192)
37. Genetic dubdivision, glacial refucia and postglacial recolonization in the golden-striped salamander, Chioglossa lusitanica (Amphibia: Urodela). Alexandrino J, Froufe E, Arntzen JW, Ferrand N, Mol Ecol 2000 9 771 781 10.1046/j.1365-294x.2000.00931.x 10849293 (Pubitemid 30354652)
38. Phylogeography and demographic history of Lacerta lepida in the Iberian Peninsula: multiple refugia, range expansions and secondary contact zones. Miraldo A, Hewitt GM, Paulo OS, Emerson BC, BMC Evol Biol 2011 11 170 10.1186/1471-2148-11-170 21682856
39. Integrative analyses of speciation and divergence in Psammodromus hispanicus (Squamata: Lacertidae). Fitze PS, González-Jimena V, San-José LM, San Mauro D, Aragón P, Suárez T, Zardoya R, BMC Evol Biol 2011 11 347 10.1186/1471-2148-11-347 22129245
40. Phylogenetic relationships and biogeography of midwife toads (Discoglossidae: Alytes). Martínez-Solano I, Gonçalves HA, Arntzen JW, García-París M, J Biogeogr 2004 31 603 618 10.1046/j.1365-2699.2003.01033.x (Pubitemid 38459059)
41. Mitochondrial DNA phylogeography of Lissotriton boscai (Caudata, Salamandridae): evidence for old, multiple refugia in an Iberian endemic. Martínez-Solano I, Teixeira D, Buckley D, García-París M, Mol Ecol 2006 15 3375 3388 10.1111/j.1365-294X.2006.03013.x 16968276 (Pubitemid 44379738)
42. High levels of population subdivision in a morphologically conserved Mediterranean toad (Alytes cisternasii) result from recent, multiple refugia: evidence from mtDNA, microsatellites and nuclear genealogies. Gonçalves H, Martínez-Solano I, Pereira RJ, Carvalho B, García-París M, Ferrand N, Mol Ecol 2009 18 5143 5160 10.1111/j.1365-294X.2009.04426.x 19912538
43. Morphological variability of the Cantabro-Pyrenean populations of Zootoca vivipara (Jaquin, 1787) with description of a new subspecies. Arribas OJ, Herpetozoa 2009 21 123 146
44. Variation in phenotype, parasite load and male competitive ability across a cryptic hybrid zone. Stuart-Fox D, Godinho R, Goüy de Bellocq J, Irwin NR, Brito JC, Moussalli A, Široký P, Hugall AF, Baird SJE, PLOS One 2009 4 5677 10.1371/journal.pone.0005677 19479073
45. Association of Mc1r variants with ecologically relevant phenotypes in the European ocellated lizard, Lacerta lepida. Nunes VL, Miraldo A, Beaumont MA, Butlin RK, Paulo OS, J Evol Biol 2011 24 2289 2298 10.1111/j.1420-9101.2011. 02359.x 21812853
46. Nuclear markers, mitochondrial DNA and male secondary sexual traits variation in a newt hybrid zone (Triturus vulgaris x T. montandoni). Babik W, Szymura JM, Rafiński J, Mol Ecol 2003 12 1913 1930 10.1046/j.1365-294X. 2003.01880.x 12803641 (Pubitemid 36815025)
47. Predictors for reproductive isolation in a ring species complex following genetic and ecological divergence. Pereira RJ, Monahan WB, Wake DB, BMC Evol Biol 2011 11 194 10.1186/1471-2148-11-194 21733173
48. A hybrid zone between two subspecies of the grasshopper Chorthippus parallelus along the Pyrenees: the west end. Buño I, Torroja E, López-Fernández C, Butlin RK, Hewitt GM, Gosálvez J, Heredity 1994 73 625 634 10.1038/hdy.1994.170
49. Song variation in the chiffchaffs (Phylloscopus collybita) of the western Pyrenees-the contact zone btween the collybita and brehmii forms. Salomon M, Hemin Y, Ethology 1992 92 265 282
50. Sex ratio and unisexual sterility in hybrid animals. Haldane J, J Genet 1922 12 101 109 10.1007/BF02983075
51. Restricted gene flow in a moving hybrid zone of the newts Triturus cristatus and T. marmoratus in western France. Arntzen JW, Wallis GP, Evolution 1991 45 805 826 10.2307/2409691
52. Hybrid populations selectively filter gene introgression between species. Martinsen GD, Whitham TG, Turek RJ, Keim P, Evolution 2001 55 1325 1335 11525457 (Pubitemid 32774051)
53. Plumage and mitochondrial DNA haplotype variation across a moving hybrid zone. Rohwer S, Bermingham E, Wood C, Evolution 2001 55 405 422 11308096 (Pubitemid 32228624)
54. Leaky prezygotic isolation and porous genomes: rapid introgression of maternally inherited DNA. Chan KMA, Levin SA, Evolution 2005 59 720 729 15926684 (Pubitemid 40589284)
55. A 2000 km genetic wake yields evidence for northern glacial refugia and hybrid zone movement in a pair of songbirds. Krosby M, Rohwer S, Proc R Soc B 2009 276 615 621 10.1098/rspb.2008.1310 18986973
56. Genetic exchange across a hybrid zone within the Iberian endemic golden-striped salamander, Chioglossa lusitanica. Sequeira F, Alexandrino J, Rocha S, Arntzen W, Ferrand N, Mol Ecol 2005 14 245 254 15643967 (Pubitemid 40141491)
57. The limits of mtDNA phylogeography: complex patterns of population fragmentation, expansion and admixture in the highly structured Iberian lizard Lacerta schreiberi are only revealed by the use of nuclear markers. Godinho R, Crespo EG, Ferrand N, Mol Ecol 2008 17 4670 4683 10.1111/j.1365-294X.2008.03929. x 18828782
58. Genetic admixture between the Iberian endemic lizards Podarcis bocagei and Podarcis carbonelli: evidence for limited natural hybridization and a bimodal hybrid zone. Pinho C, Kaliontzopoulou A, Carretero MA, Harris DJ, Ferrand N, J Zool Syst Evol Res 2009 47 368 377 10.1111/j.1439-0469.2009.00532.x
59. Hybridization as an invasion of the genome. Mallet J, Trends Ecol Evol 2005 20 229 237 10.1016/j.tree.2005.02.010 16701374 (Pubitemid 40588907)
60. Variation in mitochondrial cytochrome b sequences in natural populations of South American akodontine rodents (Muridae: Sigmodontinae). Smith MF, Patton JL, Mol Biol Evol 1991 8 85 103 2002767 (Pubitemid 21895884)
61. Two novel gene orders and the role of light-strand replication in rearrangement of the vertebrate mitochondrial genome. Macey JR, Larson A, Ananjeva NB, Fang ZL, Papenfuss TJ, Mol Biol Evol 1997 14 91 104 10.1093/oxfordjournals.molbev.a025706 9000757 (Pubitemid 27024432)
62. DnaSP, DNA polymorphism analyses by the coalescent and other methods. Rozas J, Sanchez-DelBarrio JC, Messeguer X, Rozas R, Bioinformatics 2003 19 2496 2497 10.1093/bioinformatics/btg359 14668244 (Pubitemid 38016658)
63. TCS: a computer program to estimate gene genealogies. Clement M, Posada D, Crandall KA, Mol Ecol 2000 9 1657 1659 10.1046/j.1365-294x.2000.01020.x 11050560
64. jModelTest: phylogenetic model averaging. Posada D, Mol Biol Evol 2008 25 1253 1256 10.1093/molbev/msn083 18397919 (Pubitemid 351882013)
65. Combined selection of partitioning schemes and substitution models for phylogenetic analyses. Lanfear R, Calcott B, Ho SYW, Guindon S, Mol Biol Evol 2012 29 1695 1701 10.1093/molbev/mss020 22319168
66. Bayesian inference of phylogeny and its impact on evolutionary biology. Huelsenbeck JP, Ronquist F, Nielsen R, Bollback JP, Science 2001 294 2310 2314 10.1126/science.1065889 11743192 (Pubitemid 33140548)
67. Rambaut D, Drummond A, MCMC Trace Analysis Package (version 1.4) 2007 Available at: http://tree.bio.ed.ac.uk/software/tracer
68. Bayesian phylogenetics with BEAUti and the BEAST 1.7. Drummond AJ, Suchard MA, Xie D, Rambaut A, Mol Biol Evol 2012 29 1969 1973 10.1093/molbev/mss075 22367748
69. A review of the geckos of the genus Hemidactylus (Squamata: Gekkonidae) from Oman based on morphology, mitochondrial and nuclear data, with descriptions of eight new species. Carranza S, Arnold EN, Zootaxa 2012 3378 1 95
70. Biogeography and evolution of European cave salamanders, Hydromantes (Urodela: Plethodontidae), inferred from mtDNA sequences. Carranza S, Romano A, Arnold EN, Sotgiu G, J Biogeogr 2008 35 724 738 10.1111/j.1365-2699.2007.01817.x (Pubitemid 351388728)
71. Divergence times and colonization of the Canary Islands by Gallotia lizards. Cox SC, Carranza S, Brown RP, Mol Phylogenet Evol 2010 56 747 757 10.1016/j.ympev.2010.03.020 20307675
72. Relationships and evolution of the North African geckos, Geckonia and Tarentola (Reptilia: Gekkonidae), based on mitochondrial and nuclear DNA sequences. Carranza S, Arnold EN, Mateo JA, Geniez M, Mol Phylogenet Evol 2002 23 244 256 10.1016/S1055-7903(02)00024-6 12069554 (Pubitemid 34703379)
73. Statistical neutrality of mutations against population growth, hitchhiking and background selection. Fu YX, Genetics 1997 147 915 925 9335623
74. Population growth makes waves in the distribution of pairwise genetic differences. Rogers AR, Harpending H, Mol Biol Evol 1992 9 552 569 1316531
75. Estimation of demographic parameters from the distribution of pairwise differences when the mutation rates vary among sites: application to human mitochondrial DNA. Schneider S, Excoffier L, Genetics 1999 152 1079 1089 10388826 (Pubitemid 29330907)
76. Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Excoffier L, Lischer HEL, Mol Ecol Resour 2010 10 564 567 10.1111/j.1755-0998.2010.02847.x 21565059
77. Marked genetic structuring and extreme dispersal limitation in the Pyrenean brook newt Calotriton asper (Amphibia: Salamandridae) revealed by genome-wide AFLP but not mtDNA. Milá B, Carranza S, Guillaume O, Clobert J, Mol Ecol 2010 19 108 120 10.1111/j.1365-294X.2009.04441.x 19943891
78. AFLP: a new technique for DNA fingerprinting. Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, et al. Nucleic Acids Res 1995 23 4407 4414 10.1093/nar/23.21.4407 7501463
79. How to track and assess genotyping errors in population genetics studies. Bonin A, Bellemain E, Bronken Eidesen P, Pompanon F, Brochmann C, Taberlet P, Mol Ecol 2004 13 3261 3273 10.1111/j.1365-294X.2004.02346.x 15487987 (Pubitemid 39415663)
80. A genome scan method to identify selected loci appropriate for both dominant and codominant markers: a Bayesian perspective. Foll M, Gaggiotti OE, Genetics 2008 180 977 993 10.1534/genetics.108.092221 18780740
81. Estimating population structure in diploids with multilocus dominant DNA markers. Zhivotovsky LA, Mol Ecol 1999 8 907 913 10.1046/j.1365-294x.1999.00620. x 10434412 (Pubitemid 29337748)
82. Analysis of population genetic structure with RAPD markers. Lynch M, Milligan BG, Mol Ecol 1994 3 91 99 10.1111/j.1365-294X.1994.tb00109.x 8019690 (Pubitemid 2073764)
83. Data from amplified fragment length polymorphism (AFLP) markers show indication of size homoplasy and of a relationship between degree of homoplasy and fragment size. Vekemans X, Beauwens T, Lemaire M, Roldán-Ruíz I, Mol Ecol 2002 11 139 151 10.1046/j.0962-1083.2001.01415.x 11903911 (Pubitemid 34108478)
84. Genalex 6: genetic analysis in excel. Population genetic software for research and teaching. Peakall R, Smouse PE, Mol Ecol Notes 2006 6 288 295 10.1111/j.1471-8286.2005.01155.x (Pubitemid 43373825)
85. Orloci L, Multivariate analysis in vegetation research The Hague: Dr W. Junk B. V 1978
86. Inference of population structure using multilocus genotype data. Pritchard JK, Stephens M, Donnelly P, Genetics 2000 155 945 959 10835412 (Pubitemid 30397141)
87. Inferring weak population structure with the assistance of sample group information. Hubisz MJ, Falush D, Stephens M, Pritchard JK, Mol Ecol Resour 2009 9 1322 1332 10.1111/j.1755-0998.2009.02591.x 21564903
88. Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Evanno G, Regnaut S, Goudet J, Mol Ecol 2005 14 2611 2620 10.1111/j.1365-294X.2005.02553.x 15969739 (Pubitemid 40886055)
89. Structure Harvester: a website and program for visualizing structure output and implementing the Evanno method. Earl D, vonHoldt BM, Conserv Gen Resour 2012 4 359 361 10.1007/s12686-011-9548-7