Green fluorescent protein (GFP)-based overexpression screening and characterization of AgrC, a receptor protein of quorum sensing in staphylococcus aureus

International Journal of Molecular Sciences

Volumn 14, Issue 9, 2013, Pages 18470-18487

  Wang, Lina ^a   Quan, Chunshan ^b,c   Liu, Baoquan ^b,c   Xu, Yongbin ^b,c   Zhao, Pengchao ^a   Xiong, Wen ^b,c   Fan, Shengdi ^b,c  

^a DALIAN INSTITUTE OF CHEMICAL PHYSICS   (China)

^b DALIAN NATIONALITIES UNIVERSITY   (China)

^c BEIJING UNIVERSITY OF POSTS AND TELECOMMUNICATIONS   (China)

Author keywords

CD spectroscopy; Detergent screening; GFP; IMAC; Membrane protein; SEC

Indexed keywords

AGRC PROTEIN; DETERGENT; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; MEMBRANE PROTEIN; POLYHISTIDINE TAG; POLYOXYETHYLENE (23) LAURYL ETHER; RECEPTOR PROTEIN; UNCLASSIFIED DRUG;

AGRC GENE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CIRCULAR DICHROISM; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME STABILITY; ENZYME STRUCTURE; FLUORESCENCE; GEL PERMEATION CHROMATOGRAPHY; GENE OVEREXPRESSION; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; NONHUMAN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; QUORUM SENSING; SCREENING TEST; SOLUBILIZATION; STAPHYLOCOCCUS AUREUS;

BACTERIAL PROTEINS; CHROMATOGRAPHY, AFFINITY; CIRCULAR DICHROISM; GREEN FLUORESCENT PROTEINS; PROTEIN KINASES; QUORUM SENSING; STAPHYLOCOCCUS AUREUS;

EID: 84883670060     PISSN: 16616596     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms140918470     Document Type: Article

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