A highly efficient site-specific integration strategy using combination of homologous recombination and the ΦC31 integrase

Journal of Biotechnology

Volumn 167, Issue 4, 2013, Pages 427-432

  Ou, Hailong ^a,c   Huang, Ying ^a,b   Ma, Qingwen ^a,b   Ren, Zhaorui ^a,b   Huang, Shuzhen ^a,b   Zeng, Fanyi ^a,b   Zeng, Yitao ^a,b  

^a SHANGHAI JIAO TONG UNIVERSITY SCHOOL OF MEDICINE   (China)

^b BEIJING HOSPITAL   (China)

^c GUIZHOU MEDICAL UNIVERSITY   (China)

Author keywords

C31 integrase; Double strand breaks; Homologous recombination; Pseudo attP site; Site specific integration

Indexed keywords

DOUBLE-STRAND BREAKS; HOMOLOGOUS RECOMBINATION; INTEGRASE; PSEUDO-ATTP SITE; SITE-SPECIFIC;

TRANSCRIPTION;

INTEGRATION;

GENOMIC DNA; INTEGRASE; NUCLEASE; THETA C31 INTEGRASE; UNCLASSIFIED DRUG;

ARTICLE; COMPARATIVE STUDY; CONTROLLED STUDY; COW; DONOR; GENE; GENE SEQUENCE; GENOME; HOMOLOGOUS RECOMBINATION; PRIORITY JOURNAL; TRANSGENICS;

DOUBLE-STRAND BREAKS; HOMOLOGOUS RECOMBINATION; PSEUDO-ATTP SITE; SITE-SPECIFIC INTEGRATION; ΦC31 INTEGRASE;

ANIMALS; CATTLE; DNA BREAKS, DOUBLE-STRANDED; GENE TARGETING; GENE TRANSFER TECHNIQUES; GENOME; HOMOLOGOUS RECOMBINATION; INTEGRASES; RECOMBINATION, GENETIC; RECOMBINATIONAL DNA REPAIR;

EID: 84883567058     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2013.08.001     Document Type: Article

References (27)

1. Beumer K.J., Trautman J.K., Bozas A., Liu J.L., Rutter J., Gall J.G., Carroll D. Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases. Proc. Natl. Acad. Sci. U.S.A. 2008, 105:19821-19826.
2. Cathomen T., Joung J.K. Zinc-finger nucleases: the next generation emerges. Mol. Ther. 2008, 16:1200-1207.
3. Chavez C.L., Keravala A., Chu J.N., Farruggio A.P., Cuéllar V.E., Voorberg J., Calos M.P. Long-term expression of human coagulation factor VIII in a tolerant mouse model using the C31 Φintegrase system. Hum. Gene Ther. 2012, 23:390-398.
4. Cristea S., Freyvert Y., Santiago Y., Holmes M.C., Urnov F.D., Gregory P.D., Cost G.J. In vivo cleavage of transgene donors promotes nuclease-mediated targeted integration. Biotechnol. Bioeng. 2012, [Epub ahead of print].
5. Gopalakrishnan K., Kumarasamy S., Abdul-Majeed S., Kalinoski A.L., Morgan E.E., Gohara A.F., Nauli S.M., Filipiak W.E., Saunders T.L., Joe B. Targeted disruption of Adamts16 gene in a rat genetic model of hypertension. Proc. Natl. Acad. Sci. U.S.A. 2012, 109:20555-20559.
6. Gupta A., Meng X., Zhu L.J., Lawson N.D., Wolfe S.A. Zinc finger protein dependent and independent contributions to the in vivo off-target activity of zinc finger nucleases. Nucleic Acids Res. 2011, 39:381-392.
7. Hu G., Goll M.G., Fisher S. ΦC31 integrase mediates efficient cassette exchange in the zebrafish germline. Dev. Dyn. 2011, 240:2101-2107.
8. Kandavelou K., Chandrasegaran S. Custom-designed molecular scissors for site-specific manipulation of the plant and mammalian genomes. Methods Mol. Biol. 2009, 544:617-636.
9. Kempe K., Rubtsova M., Berger C., Kumlehn J., Schollmeier C., Gils M. Transgene excision from wheat chromosomes by phage phiC31 integrase. Plant Mol. Biol. 2010, 72:673-687.
10. Kim Y.G., Cha J., Chandrasegaran S. Hybrid restriction enzymes: zinc finger fusions to Fok I cleavage domain. Proc. Natl. Acad. Sci. U.S.A. 1996, 93:1156-1160.
11. Li T., Huang S., Jiang W.Z., Wright D., Spalding M.H., Weeks D.P., Yang B. TAL nucleases (TALNs): hybrid proteins composed of TAL effectors and FokI DNA-cleavage domain. Nucleic Acids Res. 2011, 39:359-372.
12. Ma Q.W., Sheng H.Q., Yan J.B., Cheng S., Huang Y., Chen-Tsai Y., Ren Z.R., Huang S.Z., Zeng Y.T. Identification of pseudo attP sites for phage phiC31 integrase in bovine genome. Biochem. Biophys. Res. Commun. 2006, 345:984-988.
13. Ou H.L., Huang Y., Qu L.J., Xu M., Yan J.B., Ren Z.R., Huang S.Z., Zeng Y.T. A phiC31 integrase-mediated integration hotspot in favor of transgene expression exists in the bovine genome. FASEB J. 2009, 276:155-163.
14. Pattanayak V., Ramirez C.L., Joung J.K., Liu D.R. Revealing off-target cleavage specificities of zinc-finger nucleases by in vitro selection. Nat. Methods 2011, 8:765-770.
15. Petek L.M., Russell D.W., Miller D.G. Frequent endonuclease cleavage at off-target locations in vivo. Mol. Ther. 2010, 18:983-986.
16. Qu L.J., Ma Q.W., Zhou Z.W., Ma H.Y., Huang Y., Huang S.Z., Zeng F.Y., Zeng Y.T. A profile of native integration sites used by ΦC31 integrase in the bovine genome. J. Genet. Genomics 2012, 39:217-224.
17. Rouet P., Smih F., Jasin M. Expression of a site-specific endonuclease stimulates homologous recombination in mammalian cells. Proc. Natl. Acad. Sci. U.S.A. 1994, 91:6064-6068.
18. Tesson L., Usal C., Ménoret S., Leung E., Niles B.J., Remy S., Santiago Y., Vincent A.I., Meng X., Zhang L., Gregory P.D., Anegon I., Cost G.J. Knockout rats generated by embryo microinjection of TALENs. Nat. Biotechnol. 2011, 29:695-696.
19. Thyagarajan B., Olivares E.C., Hollis R.P., Ginsburg D.S., Calos M.P. Site-specific genomic integration in mammalian cells mediated by phage phiC31 integrase. Mol. Cell Biol. 2001, 21:3926-3934.
20. Townsend J.A., Wright D.A., Winfrey R.J., Fu F., Maeder M.L., Joung J.K., Voytas D.F. High-frequency modification of plant genes using engineered zinc-finger nucleases. Nature 2009, 459:442-445.
21. Urnov F.D., Miller J.C., Lee Y.L., Beausejour C.M., Rock J.M., Augustus S., Jamieson A.C., Porteus M.H., Gregory P.D., Holmes M.C. Highly efficient endogenous human gene correction using designed zinc-finger nucleases. Nature 2005, 435:646-651.
22. van der Weyden L., Adams D.J., Bradley A. Tools for targeted manipulation of the mouse genome. Physiol. Genomics 2002, 11:133-164.
23. Vasquez K.M., Marburger K., Intody Z., Wilson J.H. Manipulating the mammalian genome by homologous recombination. Proc. Natl. Acad. Sci. U.S.A. 2001, 98:8403-8410.
24. Venken K.J., Schulze K.L., Haelterman N.A., Pan H., He Y., Evans-Holm M., Carlson J.W., Levis R.W., Spradling A.C., Hoskins R.A., Bellen H.J. MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes. Nat. Methods 2011, 8:737-743.
25. Wood A.J., Lo T.W., Zeitler B., Pickle C.S., Ralston E.J., Lee A.H., Amora R., Miller J.C., Leung E., Meng X., Zhang L., Rebar E.J., Gregory P.D., Urnov F.D., Meyer B.J. Targeted genome editing across species using ZFNs and TALENs. Science 2011, 33:307.
26. Zhang F., Cong L., Lodato S., Kosuri S., Church G.M., Arlotta P. Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat. Biotechnol. 2011, 29:149-153.
27. Zhang F., Maeder M.L., Unger-Wallace E., Hoshaw J.P., Reyon D., Christian M., Li X., Pierick C.J., Dobbs D., Peterson T., Joung J.K., Voytas D.F. High frequency targeted mutagenesis in Arabidopsis thaliana using zinc finger nucleases. Proc. Natl. Acad. Sci. U.S.A. 2010, 107:12028-12033.