Transgene excision from wheat chromosomes by phage phiC31 integrase

Plant Molecular Biology

Volumn 72, Issue 6, 2010, Pages 673-687

  Kempe, Katja ^a   Rubtsova, Myroslava ^a   Berger, Carolin ^a   Kumlehn, Jochen ^a   Schollmeier, Corinna ^a   Gils, Mario ^a  

^a LEIBNIZ INSTITUTE OF PLANT GENETICS AND CROP PLANT RESEARCH IPK   (Germany)

Author keywords

Site specific recombination; Streptomyces phage phic31 integrase; Transgene excision; Transgenic wheat

Indexed keywords

STREPTOMYCES; TRITICUM AESTIVUM;

INTEGRASE; VIRUS PROTEIN;

ARTICLE; BACTERIOPHAGE; DNA SEQUENCE; ENZYMOLOGY; GENETIC ENGINEERING; GENETIC RECOMBINATION; GENETICS; HYBRIDIZATION; METHODOLOGY; PHYSIOLOGY; PLANT CHROMOSOME; TRANSGENE; WHEAT;

BACTERIOPHAGES; CHROMOSOMES, PLANT; GENETIC ENGINEERING; HYBRIDIZATION, GENETIC; INTEGRASES; RECOMBINATION, GENETIC; SEQUENCE ANALYSIS, DNA; TRANSGENES; TRITICUM; VIRAL PROTEINS;

EID: 77954468536     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11103-010-9606-7     Document Type: Article

References (57)

1. Albert H, Dale EC, Lee E, Ow DW (1995) Site-specific integration of DNA into wild-type and mutant lox sites placed in the plant genome. Plant J 7:649-659
2. Allen BG, Weeks DL (2006) Using phiC31 integrase to make transgenic Xenopus laevis embryos. Nat Protoc 1:1248-1257
3. Andreas S, Schwenk F, Kuter-Luks B, Faust N, Kuhn R (2002) Enhanced efficiency through nuclear localization signal fusion on phage PhiC31-integrase: activity comparison with Cre and FLPe recombinase in mammalian cells. Nucleic Acids Res 30:2299-2306
4. Araki K, Araki M, Yamamura K (1997) Targeted integration of DNA using mutant lox sites in embryonic stem cells. Nucleic Acids Res 25:868-872
5. Bateman JR, Lee AM, Wu CT (2006) Site-specific transformation of Drosophila via phiC31 integrase-mediated cassette exchange. Genetics 173:769-777
6. Baubonis W, Sauer B (1993) Genomic targeting with purified Cre recombinase. Nucleic Acids Res 21:2025-2029
7. Bischof J, Basler K (2008) Recombinases and their use in gene activation, gene inactivation, and transgenesis. Methods Mol Biol 420:175-195
8. Chawla R, Ariza-Nieto M, Wilson AJ, Moore SK, Srivastava V (2006) Transgene expression produced by biolistic-mediated, site-specific gene integration is consistently inherited by the subsequent generations. Plant Biotechnol J 4:209-218
9. Christensen AH, Quail PH (1996) Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants. Transgenic Res 5:213-218
10. Coppoolse ER, de Vroomen MJ, Roelofs D, Smit J, van Gennip F, Hersmus BJ, Nijkamp HJ, van Haaren MJ (2003) Cre recombinase expression can result in phenotypic aberrations in plants. Plant Mol Biol 51:263-279
11. Coppoolse ER, de Vroomen MJ, van Gennip F, Hersmus BJ, van Haaren MJ (2005) Size does matter: cre-mediated somatic deletion efficiency depends on the distance between the target lox-sites. Plant Mol Biol 58:687-698
12. De Buck S, Peck I, De Wilde C, Marjanac G, Nolf J, De Paepe A, Depicker A (2007) Generation of single-copy T-DNA transformants in Arabidopsis by the CRE/loxP recombination-mediated resolution system. Plant Physiol 145:1171-1182
13. Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II Plant Mol Biol. Pep 1:19-29
14. Gielen J, De Beuckeleer M, Seurinck J, Deboeck F, De Greve H, Lemmers M, Van Montagu M, Schell J (1984) The complete nucleotide sequence of the TL-DNA of the Agrobacterium tumefaciens plasmid pTiAch5. EMBO J 3:835-846
15. Gierl A, Schwarz-Sommer Z, Saedler H (1985) Molecular interactions between the components of the En-I transposable element system of Zea mays. EMBO J 4:579-583
16. Gilbertson L (2003) Cre-lox recombination: cre-ative tools for plant biotechnology. Trends Biotechnol 21:550-555
17. Gils M, Marillonnet S, Werner S, Grutzner R, Giritch A, Engler C, Schachschneider R, Klimyuk V, Gleba Y (2008) A novel hybrid seed system for plants. Plant Biotechnol J 6:226-235
18. Ginsburg DS, Calos MP (2005) Site-specific integration with phiC31 integrase for prolonged expression of therapeutic genes. Adv Genet 54:179-187
19. Gleba Y, Marillonnet S, Klimyuk V (2004) Design of safe and biologically contained transgenic plants: tools and technologies for controlled transgene flow and expression. Biotechnol Genet Eng Rev 21:325-367
20. Grindley ND, Whiteson KL, Rice PA (2006) Mechanisms of sitespecific recombination. Annu Rev Biochem 75:567-605
21. Gronlund JT, Stemmer C, Lichota J, Merkle T, Grasser KD (2007) Functionality of the beta/six site-specific recombination system in tobacco and Arabidopsis: a novel tool for genetic engineering of plant genomes. Plant Mol Biol 63:545-556
22. Hare PD, Chua NH (2002) Excision of selectable marker genes from transgenic plants. Nat Biotechnol 20:575-580
23. Heidmann D, Lehner CF (2001) Reduction of Cre recombinase toxicity in proliferating Drosophila cells by estrogen-dependent activity regulation. Dev Genes Evol 211:458-465
24. Hensel G, Kastner C, Oleszczuk S, Riechen J, Kumlehn J (2009) Agrobacterium-mediated gene transfer to cereal crop plants: current protocols for barley, wheat, triticale, and maize. Int J Plant Genomics 2009:1-9
25. Hoa TT, Bong BB, Huq E, Hodges TK (2002) Cre/ lox site-specific recombination controls the excision of a transgene from the rice genome. Theor Appl Genet 104:518-525
26. Jones JD, Shlumukov L, Carland F, English J, Scofield SR, Bishop GJ, Harrison K (1992) Effective vectors for transformation, expression of heterologous genes, and assaying transposon excision in transgenic plants. Transgenic Res 1:285-297
27. Kempe K, Rubtsova M, Gils M (2009) Intein-mediated protein assembly in transgenic wheat: production of active barnase and acetolactate synthase from split genes. Plant Biotechnol J 7: 283-297
28. Kilby NJ, Snaith MR, Murray JA (1993) Site-specific recombinases: tools for genome engineering. Trends Genet 9:413-421
29. Kittiwongwattana C, Lutz K, Clark M, Maliga P (2007) Plastid marker gene excision by the phiC31 phage site-specific recombinase. Plant Mol Biol 64:137-143
30. Loonstra A, Vooijs M, Beverloo HB, Al Allak B, van Drunen E, Kanaar R, Berns A, Jonkers J (2001) Growth inhibition and DNA damage induced by Cre recombinase in mammalian cells. Proc Natl Acad Sci USA 98:9209-9214
31. Luo H, Lyznik LA, Gidoni D, Hodges TK (2000) FLP-mediated recombination for use in hybrid plant production. Plant J 23:423-430
32. Lutz KA, Corneille S, Azhagiri AK, Svab Z, Maliga P (2004) A novel approach to plastid transformation utilizes the phiC31 phage integrase. Plant J 37:906-913
33. Lyznik LA, Gordon-Kamm WJ, Tao Y (2003) Site-specific recombination for genetic engineering in plants. Plant Cell Rep 21:925-932
34. Maeser S, Kahmann R (1991) The Gin recombinase of phage Mu can catalyse site-specific recombination in plant protoplasts. Mol Gen Genet 230:170-176
35. Mariani C, De Beuckeleer M, Truettner J, Leemans J, Goldberg RB (1990) Induction of male sterility in plants by a chimaeric ribonuclease gene. Nature 374:737-741
36. McElroy D, Zhang W, Cao J, Wu R (1990) Isolation of an efficient actin promoter for use in rice transformation. Plant Cell 2: 163-171
37. Ow DW, Medberry SL (1995) Genome manipulation through site-specific recombination. CRC Crit Rev Plant Sci 14: 239-261
38. Mengiste T, Revenkova E, Bechtold N, Paszkowski J (1999) An SMC-like protein is required for efficient homologous recombination in Arabidopsis. EMBO J 18:4505-4512
39. Mlynarova L, Nap JP (2003) A self-excising Cre recombinase allows efficient recombination of multiple ectopic heterospecific lox sites in transgenic tobacco. Transgenic Res 12:45-57
40. Ow DW (2002) Recombinase-directed plant transformation for the post-genomic era. Plant Mol Biol 48:183-200
41. Ow DW (2007) GM maize from site-specific recombination technology, what next? Curr Opin Biotechnol 18:115-120
42. Puchta H (2003) Towards the ideal GMP: homologous recombination and marker gene excision. J Plant Physiol 160:743-754
43. Que Q, Wang HR, Jorgensen A (1998) Distinct patterns of pigment suppression are produced by allelic sense and antisense chalcone synthase transgenes in petunia flowers. Plant J 13:401-409
44. Rubtsova M, Kempe K, Gils A, Ismagul A, Weyen J, Gils M (2008) Expression of active Streptomyces phage phiC31 integrase in transgenic wheat plants. Plant Cell Rep 27:1821-1831
45. Shamay I (2005) Method of producing a male sterile plant by exogenic allelism. US Patent 6852911, US2005066388, WO0116287, EP1209967 FERTISEEDS LTD [IR]
46. Smith MC, Thorpe HM (2002) Diversity in the serine recombinases. Mol Microbiol 44:299-307
47. Smith MC, Till R, Brady K, Soultanas P, Thorpe H (2004) Synapsis and DNA cleavage in phiC31 integrase-mediated site-specific recombination. Nucleic Acids Res 32:2607-2617
48. Southern EM (1992) Detection of specific sequences among DNA fragments separated by gel electrophoresis. 1975. Biotechnology 24:122-139
49. Srivastava V, Ow DW (2001) Single-copy primary transformants of maize obtained through the co-introduction of a recombinaseexpressing construct. Plant Mol Biol 46:561-566
50. Srivastava V, Anderson OD, Ow DW (1999) Single-copy transgenic wheat generated through the resolution of complex integration patterns. Proc Natl Acad Sci U S A 96:11117-11121
51. Srivastava V, Ariza-Nieto M, Wilson AJ (2004) Cre-mediated sitespecific gene integration for consistent transgene expression in rice. Plant Biotechnol J 2:169-179
52. Thorpe HM, Smith MC (1998) In vitro site-specific integration of bacteriophage DNA catalyzed by a recombinase of the resolvase/invertase family. Proc Natl Acad Sci U S A 95:5505-5510
53. Tsuchiya T, Toriyama K, Yoshikawa M, Ejiri S, Hinata K (1995) Tapetum-specific expression of the gene for an endo-beta-1, 3-glucanase causes male sterility in transgenic tobacco. Plant Cell Physiol 36:487-494
54. Tungsuchat T, Kuroda H, Narangajavana J, Maliga P (2006) Gene activation in plastids by the CRE site-specific recombinase. Plant Mol Biol 61:711-718
55. Venken KJ, He Y, Hoskins RA, Bellen HJ (2006) P[acman]: a BAC transgenic platform for targeted insertion of large DNA fragments in D. melanogaster. Science 314:1747-1751
56. Vergunst AC, Jansen LE, Hooykaas PJ (1998) Site-specific integration of Agrobacterium T-DNA in Arabidopsis thaliana mediated by Cre recombinase. Nucleic Acids Res 26:2729-2734
57. Wirth D, Gama-Norton L, Riemer P, Sandhu U, Schucht R, Hauser H (2007) Road to precision: recombinase-based targeting technologies for genome engineering. Curr Opin Biotechnol 18:411-41