Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions

Nature Protocols

Volumn 8, Issue 9, 2013, Pages 1787-1799

  Guimaraes, Carla P ^a   Witte, Martin D ^a,c   Theile, Christopher S ^a   Bozkurt, Gunes ^a   Kundrat, Lenka ^a   Blom, Annet E M ^a   Ploegh, Hidde L ^a,b  

^a WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^c UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

BIOTIN; FLUOROCHROME; GLYCINE; LIPID; OLIGOGLYCINE PEPTIDE; PEPTIDE; PROTEIN; SORTASE; SORTASE A; THREONINE; UNCLASSIFIED DRUG;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CHEMICAL LABELING; CHEMICAL REACTION; CONTROLLED STUDY; ENZYME PURIFICATION; ENZYME STRUCTURE; INTERNAL LOOP; MOLECULAR RECOGNITION; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN MODIFICATION; PROTEIN STRUCTURE; REACTION TIME; RECOGNITION SEQUENCE; SITE SPECIFIC LABELING; SORTASE RECOGNITION MOTIF; TRANSPEPTIDATION;

AMINO ACID SEQUENCE; AMINOACYLTRANSFERASES; BACTERIAL PROTEINS; CELL WALL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, LIQUID; CYSTEINE ENDOPEPTIDASES; MASS SPECTROMETRY; PROTEIN ENGINEERING;

EID: 84883428060     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2013.101     Document Type: Article

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