Systematic screening of glycosylation- and trafficking-associated gene knockouts in Saccharomyces cerevisiae identifies mutants with improved heterologous exocellulase activity and host secretion

BMC Biotechnology

Volumn 13, Issue , 2013, Pages

  Wang, Tzi Yuan ^a   Huang, Chih Jen ^a,b   Chen, Hsin Liang ^a   Ho, Po Chun ^a   Ke, Huei Mien ^a,b   Cho, Hsing Yi ^b,c   Ruan, Sz Kai ^a   Hung, Kuo Yen ^a   Wang, I Li ^a   Cai, Ya Wun ^a   Sung, Huang Mo ^d   Li, Wen Hsiung ^a,e   Shih, Ming Che ^b,c  

^a BIODIVERSITY RESEARCH CENTER   (Taiwan)

^b NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

^c AGRICULTURAL BIOTECHNOLOGY RESEARCH CENTER   (Taiwan)

^d NATIONAL CHENG KUNG UNIVERSITY   (Taiwan)

^e UNIVERSITY OF CHICAGO   (United States)

Author keywords

Cellulase production; Glycosylation; Protein secretion

Indexed keywords

CELLULASE PRODUCTION; CONSOLIDATED BIO-PROCESSING; EXTRACELLULAR ACTIVITY; EXTRACELLULAR CELLULASE; HETEROLOGOUS PROTEINS; PHANEROCHAETE CHRYSOSPORIUM; PROTEIN SECRETION; SITE DIRECTED MUTAGENESIS;

CELLULOSE; ENZYME ACTIVITY; ESTERIFICATION; GLYCOSYLATION; PHYSIOLOGY; PROTEINS; YEAST;

GENE EXPRESSION;

CELLULASE; CELLULOSE; EXOCELLULASE; UNCLASSIFIED DRUG;

ARTICLE; DEGLYCOSYLATION; ENDOSOME; ENZYME ACTIVITY; GENETIC SCREENING; GLYCOSYLATION; KNOCKOUT GENE; NONHUMAN; PHANEROCHAETE; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; PROTEIN MODIFICATION; SACCHAROMYCES CEREVISIAE; SITE DIRECTED MUTAGENESIS; WILD TYPE;

FUNGI; PHANEROCHAETE CHRYSOSPORIUM; SACCHAROMYCES CEREVISIAE;

CELLULASES; CELLULOSE; ETHANOL; FUNGAL PROTEINS; GENE KNOCKOUT TECHNIQUES; GLYCOSYLATION; MUTAGENESIS, SITE-DIRECTED; PHANEROCHAETE; PROTEIN TRANSPORT; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE;

EID: 84883271760     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-13-71     Document Type: Article

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