An acidic and thermostable carboxymethyl cellulase from the yeast Cryptococcus sp. S-2: Purification, characterization and improvement of its recombinant enzyme production by high cell-density fermentation of Pichia pastoris

Protein Expression and Purification

Volumn 60, Issue 2, 2008, Pages 140-146

  Thongekkaew, Jantaporn ^a   Ikeda, Hiroko ^b   Masaki, Kazuo ^b   Iefuji, Haruyuki ^b  

^a Department of Mathematics   (Thailand)

^b NATIONAL RESEARCH INSTITUTE OF BREWING   (Japan)

Author keywords

Acidic cellulase; Cryptococcus sp. S 2; High cell density fermentation; Pichia pastoris; Thermostable cellulase

Indexed keywords

CARBOXYMETHYLCELLULASE; CELLULASE; COMPLEMENTARY DNA; PRIMER DNA; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; CHEMISTRY; CRYPTOCOCCUS; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYMOLOGY; FERMENTATION; GEL CHROMATOGRAPHY; GENETICS; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PH; PLASMID; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

AMINO ACID SEQUENCE; BASE SEQUENCE; CATALYSIS; CELLULASE; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; CRYPTOCOCCUS; DNA PRIMERS; DNA, COMPLEMENTARY; ENZYME STABILITY; FERMENTATION; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; PLASMIDS; RECOMBINANT PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SUBSTRATE SPECIFICITY;

CRYPTOCOCCUS SP. S-2; PICHIA PASTORIS;

EID: 45449118076     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2008.03.021     Document Type: Article

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