Phosphatidylethanol: The potential role in further evaluating low positive urinary ethyl glucuronide and ethyl sulfate results

Alcoholism: Clinical and Experimental Research

Volumn 37, Issue 9, 2013, Pages 1582-1586

  Skipper, Gregory E ^a   Thon, Natasha ^b   Dupont, Robert L ^c   Baxter, Louis ^d   Wurst, Friedrich M ^b  

^a Promises Treatment Centers   (United States)

^b PARACELSUS MEDICAL UNIVERSITY   (Austria)

^c Institute for Behavior and Health   (United States)

^d NONE

Author keywords

Ethyl Glucuronide; Ethyl Sulfate; Monitoring; Phosphatidylethanol; Physician Health Program

Indexed keywords

ALCOHOL; ETHYL GLUCURONIDE; ETHYL SULFATE; PHOSPHATIDYLETHANOL; SULFATE; UNCLASSIFIED DRUG;

ALCOHOL CONSUMPTION; ARTICLE; BIOLOGICAL MONITORING; CLINICAL ARTICLE; HUMAN; LABORATORY TEST; LIQUID CHROMATOGRAPHY; PRIORITY JOURNAL; TANDEM MASS SPECTROMETRY;

ETHYL GLUCURONIDE; ETHYL SULFATE; MONITORING; PHOSPHATIDYLETHANOL; PHYSICIAN HEALTH PROGRAM;

ALABAMA; ALCOHOL DRINKING; BIOLOGICAL MARKERS; FEMALE; GLUCURONATES; GLYCEROPHOSPHOLIPIDS; HUMANS; MALE; PILOT PROJECTS; SULFURIC ACID ESTERS;

EID: 84883240084     PISSN: 01456008     EISSN: 15300277     Source Type: Journal    
DOI: 10.1111/acer.12121     Document Type: Article

References (37)

1. Allen JP, Marques P, Wurst F (2009) Biomarkers of alcohol use: their nature, strengths, and limitations. Mil Med 173:v-viii.
2. Aradottir S, Asanovska G, Gjerss S, Hansson P, Alling C (2006) Phosphatidylethanol (PEth) concentrations in blood are correlated to reported alcohol intake in alcohol-dependent patients. Alcohol Alcohol 41:431-437.
3. Aradóttir S, Seidl S, Wurst FM, Jönsson BA, Alling C (2004) Phosphatidylethanol in human organs and blood: a study on autopsy material and influences by storage conditions. Alcohol Clin Exp Res 28:1718-1723.
4. Baranowski S, Serr A, Thierauf A, Weinmann W, Perdekamp G, Wurst FM, Halter CC (2008) In vitro study of abacterial degradation of ethyl glucuronide and ethyl sulphate. Int J Legal Med 122:389-393.
5. Böttcher M, Beck O, Helander A (2008) Evaluation of a new immunoassay for urinary ethyl glucuronide testing. Alcohol Alcohol 43:46-48.
6. Conigrave KM, Degenhardt LJ, Whitfield B, Saunders B, Helander A, Tabakoff B (2002) On behalf of the WHO/ISBRA study on biological state and trait markers of alcohol use and dependence investigators. CDT, GGT, and AST as markers of alcohol use: the WHO/ISBRA collaborative project. Alcohol Clin Exp Res 26:332-339.
7. Faller A, Richter B, Kluge M, Koenig P, Seitz HK, Thierauf A, Gnann H, Winkler M, Mattern R, Skopp G (2011) LC-MS/MS analysis of phosphatidylethanol in dried blood spots versus conventional blood specimens. Anal Bioanal Chem 401:1163-1166.
8. Gnann H, Engelmann C, Skopp G, Winkler M, Auwärter V, Dresen S, Ferreiros N, Wurst FM, Weinmann W (2010) Identification of 48 homologues of phosphatidylethanol in blood by LC-ESI-MS/MS. Anal Bioanal Chem 396:2415-2423.
9. Gnann H, Weinmann W, Thierauf A (2012) Formation of phosphatidylethanol and its subsequent elimination during an extensive drinking experiment over 5 days. Alcohol Clin Exp Res 36:1507-1511.
10. Gustavsson L, Alling C (1987) Formation of phosphatidylethanol in rat brain by phospholipase D. Biochem Biophys Res Commun 142:958-963.
11. Hannuksela ML, Liisanantti MK, Nissinen AE, Savolainen MJ (2007) Biochemical markers of alcoholism. Clin Chem Lab Med 45:953-961.
12. Hansson P, Caron M, Johnson G, Gustavsson L, Alling C (1997) Blood phosphatidylethanol of alcoholic abuse: levels in alcoholic males during withdrawal. Alcohol Clin Exp Res 21:108-110.
13. Hartmann S, Aradottir S, Graf M, Wiesbeck G, Lesch O, Ramskogler K, Wolfersdorf M, Alling C, Wurst FM (2007) Phosphatidylethanol as a sensitive and specific biomarker-comparison with gamma glutamyl transpeptidase, mean corpuscular volume and carbohydrate-deficient transferrin. Addict Biol 12:81-84.
14. Helander A (2003) Biological markers in alcoholism. J Neural Transm Suppl 66:15-32.
15. Helander A, Beck O (2005) Ethyl sulfate: a metabolite of ethanol in humans and a potential biomarker of acute alcohol intake. J Anal Toxicol 29:270-274.
16. Helander A, Böttcher M, Fehr C, Dahmen N, Beck O (2009) Detection times for urinary ethyl glucuronide and ethyl sulfate in heavy drinkers during alcohol detoxification. Alcohol Alcohol 44:55-61.
17. Helander A, Olsson I, Dahl H (2007) Postcollection synthesis of ethyl glucuronide by bacteria in urine may cause false identification of alcohol consumption. Clin Chem 53:1855-1857.
18. Helander A, Zheng Y (2009) Molecular species of the alcohol biomarker phosphatidylethanol in human blood measured by LC-MS. Clin Chem 55:1395-1405.
19. Hoiseth G, Yttredal B, Karinen R, Gjerde H, Christophersen A (2010) Levels of ethyl glucuronide and ethyl sulfate in oral fluid, blood and urine after use of mouthwash and ingestion of nonalcoholic wine. J Anal Toxicol 34:84-88.
20. Kobayashi M, Kanfer JN (1987) Phosphatidylethanol formation via trans-phosphatidylation by rat brain synaptosomal phospholipase D. J Neurochem 48:1597-1603.
21. Laposata M (1999) Assessment of ethanol intake-current tests and new assays on the horizon. Am J Clin Pathol 112:443-450.
22. Musshoff F, Albermann E, Madea B (2010) Ethyl glucuronide and ethyl sulfate in urine after consumption of various beverages and foods-misleading results? Int J Legal Med 124:623-630.
23. Rohrig TP, Huber C, Goodson L, Ross W (2006) Detection of ethyl glucuronide in urine following the application of Germ-X. J Anal Toxicol 30:703-704.
24. SAMHSA (2012) The Role of Biomarkers in the Treatment of Alcohol Use Disorders. Substance Abuse Treatment: Advisory-News for the Treatment Filed. Substance Abuse and Mental Health Services Administration. Volume 11 Issue 2. Available at: www.kap.samhsa.gov/products/manuals/advisory/pdfs/Advisory_Biomarkers_Revision.pdf. Accessed August 28, 2012.
25. Skipper GE, Weinmann W, Theirauf A, Schaefer P, Wiesbeck G, Allen JP, Miller M, Wurst FM (2004) Ethyl glucuronide: a biomarker to identify alcohol use by health professionals recovering from substance use disorders. Alcohol Alcohol 39:445-449.
26. Stephanson N, Dahl H, Helander A, Beck O (2002) Direct quantification of ethyl glucuronide in clinical urine samples by liquid chromatography-mass spectrometry. Ther Drug Monit 24:645-651.
27. Stewart SH, La TL, Randall PK, Newman R (2010) Phosphatidylethanol and alcohol consumption in reproductive age women. Alcohol Clin Exp Res 34:488-492.
28. Stewart SH, Reuben A, Brzezinski WA, Koch DG, Basile J, Randall PK, Miller PM (2009) Preliminary evaluation of phosphatidylethanol and alcohol consumption in patients with liver disease and hypertension. Alcohol Alcohol 44:464-467.
29. Thierauf A, Halter CC, Rana S, Auwaerter V, Wohlfarth A, Wurst FM, Weinmann W (2009) Urine tested positive for ethyl glucuronide after trace amounts of ethanol. Addiction 104:2007-2012.
30. Thierauf A, Wohlfarth A, Auwarter V, Perdekamp MG, Wurst FM, Weinmann W (2010) Urine tested positive for ethyl glucuronide and ethyl sulfate after the consumption of yeast and sugar. Forensic Sci Int 202:45-47.
31. Varga A, Hansson P, Johnson G, Alling C (2000) Normalization rate and cellular localization of phosphatidylethanol in whole blood from chronic alcoholics. Clin Chim Acta 299:141-150.
32. Varga A, Hansson P, Lundqvist C, Alling C (1998) Phosphatidylethanol in blood as a marker of ethanol consumption in healthy volunteers: comparison with other markers. Alcohol Clin Exp Res 22:1832-1837.
33. Walsham NE, Sherwood RA (2012) Ethyl glucuronide. Ann Clin Biochem 49:110-117.
34. Wurst FM, Alexson S, Wolfersdorf M, Bechtel G, Forster S, Alling C, Aradóttir S, Jachau K, Huber P, Allen JP, Auwärter V, Pragst F (2004) Concentration of fatty acid ethyl esters in hair of alcoholics: comparison to other biological state markers and self reported-ethanol intake. Alcohol Alcohol 39:33-38.
35. Wurst FM, Thon N, Aradottir S, Hartmann S, Wiesbeck GA, Lesch O, Skala K, Wolfersdorf M, Weinmann W, Alling C (2010) Phosphatidylethanol: normalization during detoxification, gender aspects and correlation with other biomarkers and self-reports. Addict Biol 15:88-95.
36. Wurst FM, Thon N, Weinmann W, Tippetts S, Marques P, Hahn JA, Alling C, Aradottir S, Hartmann S, Lakshman R (2012) Characterization of sialic acid index of plasma apolipoprotein J and phosphatidylethanol during alcohol detoxification-a pilot study. Alcohol Clin Exp Res 36:251-257.
37. Wurst FM, Vogel R, Jachau K, Varga A, Alling C, Alt A, Skipper GE (2003) Ethyl glucuronide discloses recent covert alcohol use not detected by standard testing in forensic psychiatric inpatients. Alcohol Clin Exp Res 27:471-476.