LC-MS/MS analysis of phosphatidylethanol in dried blood spots versus conventional blood specimens

Analytical and Bioanalytical Chemistry

Volumn 401, Issue 4, 2011, Pages 1163-1166

  Faller, Andrea ^a   Richter, Barbara ^b   Kluge, Matthias ^b   Koenig, Patrick ^c   Seitz, Helmut K ^c   Thierauf, Annette ^d   Gnann, Heike ^d   Winkler, Michaela ^e   Mattern, Rainer ^a   Skopp, Gisela ^a  

^a UNIVERSITY HOSPITAL HEIDELBERG   (Germany)

^b Psychiatric Center Nordbaden   (Germany)

^c Krankenhaus Salem   (Germany)

^d UNIVERSITY OF FREIBURG   (Germany)

^e UNIVERSITY OF ULM   (Germany)

Author keywords

Dried blood spots; LC MS MS; Phosphatidylethanol; Whole blood

Indexed keywords

ANALYTES; BLAND AND ALTMAN ANALYSIS; BLOOD SPECIMENS; DRIED BLOOD SPOTS; INTERNAL STANDARDS; LC-MS/MS; LIQUID-LIQUID EXTRACTION; MEAN VALUES; MULTIPLE REACTION MONITORING; PHOSPHATIDYLCHOLINE; PHOSPHATIDYLETHANOL; PHOSPHOLIPASE D; WHOLE BLOOD;

DETOXIFICATION; ETHANOL; LIQUIDS; MONITORING; SOLVENT EXTRACTION;

BLOOD;

BIOLOGICAL MARKER; GLYCEROPHOSPHOLIPID; PHOSPHATIDYLETHANOL;

ALCOHOLISM; ARTICLE; BLOOD; BLOOD ANALYSIS; DRIED BLOOD SPOT TESTING; HUMAN; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METHODOLOGY;

ALCOHOLISM; BIOLOGICAL MARKERS; BLOOD CHEMICAL ANALYSIS; CHROMATOGRAPHY, LIQUID; DRIED BLOOD SPOT TESTING; GLYCEROPHOSPHOLIPIDS; HUMANS; MASS SPECTROMETRY;

EID: 80052600113     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-011-5221-y     Document Type: Article

References (13)

1. S Aradottir S Seidl FM Wurst BA Jonsson C Alling 2004 Phosphatidylethanol in human organs and blood: a study on autopsy material and influences by storage conditions Alcohol Clin Exp Res 28 1718 1723 10.1097/01.ALC.0000145687. 41646.E5 1:CAS:528:DC%2BD2cXpsl2isr4%3D (Pubitemid 39551833)
2. H Gnann W Weinmann C Engelmann FM Wurst G Skopp M Winkler, et al. 2009 Selective detection of phosphatidylethanol homologues in blood as biomarkers for alcohol consumption by LC-ESI-MS/MS J Mass Spectrom 44 1293 1299 10.1002/jms.1608 1:CAS:528:DC%2BD1MXhtFGkt7fL
3. S Aradottir BL Olsson 2005 Methodological modifications on quantification of phosphatidylethanol in blood from humans abusing alcohol, using high-performance liquid chromatography and evaporative light scattering detection BMC Biochem 6 18 10.1186/1471-2091-6-18 (Pubitemid 41433784)
4. G Skopp L Potsch 2001 Detection of cocaine in blood stains Arch Kriminol 207 81 88 1:STN:280:DC%2BD3MzltVOqtA%3D%3D
5. R Garcia Boy J Henseler R Mattern G Skopp 2008 Determination of morphine and 6-acetylmorphine in blood with use of dried blood spots Ther Drug Monit 30 733 739 10.1097/FTD.0b013e31818d9fdb 1:CAS:528:DC%2BD1cXhsVKnt73F
6. Services USDoHaH, Administration FaD, (CDER) CfDEaR, (CVM) CfVM (2001) Guidance for Industry-Bioanalytical Method Validation
7. S Mantha MF Roizen LA Fleisher R Thisted J Foss 2000 Comparing methods of clinical measurement: reporting standards for Bland and Altman analysis Anesth Analg 90 593 602 10.1097/00000539-200003000-00018 1:STN:280: DC%2BD3c7mvFGhtA%3D%3D (Pubitemid 30117518)
8. A Nalesso G Viel G Cecchetto G Frison SD Ferrara 2010 Analysis of the alcohol biomarker phosphatidylethanol by NACE with on-line ESI-MS Electrophoresis 31 1227 1233 10.1002/elps.200900430 1:CAS:528: DC%2BC3cXktFyrs7k%3D
9. K Leidl G Liebisch D Richter G Schmitz 2008 Mass spectrometric analysis of lipid species of human circulating blood cells Biochim Biophys Acta 1781 655 664 1:CAS:528:DC%2BD1cXhtFymu73M
10. V Jakobik I Burus T Decsi 2009 Fatty acid composition of erythrocyte membrane lipids in healthy subjects from birth to young adulthood Eur J Pediatr 168 141 147 10.1007/s00431-008-0719-9 1:CAS:528:DC%2BD1cXhsFWrsrrJ
11. JV Mei JR Alexander BW Adam WH Hannon 2001 Use of filter paper for the collection and analysis of human whole blood specimens J Nutr 131 1631 1637
12. SP Parker WD Cubitt 1999 The use of the dried blood spot sample in epidemiological studies J Clin Pathol 52 633 639 10.1136/jcp.52.9.633 1:STN:280:DC%2BD3c7itFCgtA%3D%3D (Pubitemid 29456898)
13. H Gnann C Engelmann G Skopp M Winkler V Auwarter S Dresen, et al. 2010 Identification of 48 homologues of phosphatidylethanol in blood by LC-ESI-MS/MS Anal Bioanal Chem 396 2415 2423 10.1007/s00216-010-3458-5 1:CAS:528: DC%2BC3cXhtl2ntLs%3D