Molecular pathology of brain edema after severe burns in forensic autopsy cases with special regard to the importance of reference gene selection

International Journal of Legal Medicine

Volumn 127, Issue 5, 2013, Pages 881-889

  Wang, Qi ^a,b   Ishikawa, Takaki ^b   Michiue, Tomomi ^b   Zhu, Bao Li ^c   Guan, Da Wei ^c   Maeda, Hitoshi ^b  

^a SOUTHERN MEDICAL UNIVERSITY   (China)

^b OSAKA CITY UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^c CHINA MEDICAL UNIVERSITY   (China)

Author keywords

Brain edema; Forensic molecular pathology; Reference gene validation; RT qPCR; Severe burns

Indexed keywords

AQUAPORIN; CLAUDIN 5; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; IPO8 PROTEIN, HUMAN; KARYOPHERIN BETA; MATRIX METALLOPROTEINASE; MESSENGER RNA; PES1 PROTEIN, HUMAN; PROTEIN; RNA POLYMERASE II;

ADOLESCENT; ADULT; AGED; ANALYSIS OF VARIANCE; ARTICLE; ASPHYXIA; BODY WATER; BRAIN; BRAIN EDEMA; BURN; COMPARATIVE STUDY; FEMALE; FIRE; FORENSIC PATHOLOGY; GENE EXPRESSION PROFILING; GENETICS; HUMAN; IMMUNOHISTOCHEMISTRY; MALE; METABOLISM; MIDDLE AGED; PATHOLOGY; REAL TIME POLYMERASE CHAIN REACTION; SUDDEN CARDIAC DEATH; VERY ELDERLY; YOUNG ADULT;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; ANALYSIS OF VARIANCE; AQUAPORINS; ASPHYXIA; BETA KARYOPHERINS; BODY WATER; BRAIN; BRAIN EDEMA; BURNS; CLAUDIN-5; DEATH, SUDDEN, CARDIAC; FEMALE; FIRES; FORENSIC PATHOLOGY; GENE EXPRESSION PROFILING; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; HUMANS; IMMUNOHISTOCHEMISTRY; MALE; MATRIX METALLOPROTEINASES; MIDDLE AGED; PROTEINS; REAL-TIME POLYMERASE CHAIN REACTION; RNA POLYMERASE II; RNA, MESSENGER; YOUNG ADULT;

EID: 84883209789     PISSN: 09379827     EISSN: 14371596     Source Type: Journal    
DOI: 10.1007/s00414-013-0868-x     Document Type: Article

References (48)

1. Jeschke MG, Chinkes DL, Finnerty CC, Kulp G, Suman OE, Norbury WB, Branski LK, Gauglitz GG, Mlcak RP, Herndon DN (2008) Pathophysiologic response to severe burn injury. Ann Surg 248(3):387-401
2. Wang Q, Ishikawa T, Michiue T, Zhu BL, Maeda H (2011) Evaluation of human brain damage in fire fatality by quantification of basic fibroblast growth factor (bFGF), glial fibrillary acidic protein (GFAP) and single-stranded DNA (ssDNA) immunoreactivities. Forensic Sci Int 211(1-3):19-26
3. Ipaktchi K, Arbabi S (2006) Advances in burn critical care. Crit Care Med 34(9 Suppl):S239-244
4. Williams FN, Herndon DN, Hawkins HK, Lee JO, Cox RA, Kulp GA, Finnerty CC, Chinkes DL, Jeschke MG (2009) The leading causes of death after burn injury in a single pediatric burn center. Crit Care 13(6):R183
5. Swann K, Berger J, Sprague SM, Wu Y, Lai Q, Jimenez DF, Barone CM, Ding Y (2007) Peripheral thermal injury causes blood-brain barrier dysfunction and matrix metalloproteinase (MMP) expression in rat. Brain Res 1129(1):26-33
6. Verkman AS (2002) Physiological importance of aquaporin water channels. Ann Med 34(3):192-200
7. Zador Z, Bloch O, Yao X, Manley GT (2007) Aquaporins: role in cerebral edema and brain water balance. Prog Brain Res 161:185-194
8. Turksen K, Troy TC (2004) Barriers built on claudins. J Cell Sci 117(Pt 12):2435-2447
9. Furuse M, Tsukita S (2006) Claudins in occluding junctions of humans and flies. Trends Cell Biol 16(4):181-188
10. Woessner JF Jr (1994) The family of matrix metalloproteinases. Ann N Y Acad Sci 732:11-21
11. Wang Q, Ishikawa T, Michiue T, Zhu BL, Guan DW, Maeda H (2012) Stability of endogenous reference genes in postmortem human brains for normalization of quantitative real-time PCR data: comprehensive evaluation using geNorm, NormFinder, and BestKeeper. Int J Legal Med 126(6):943-952
12. Zhao S, Fernald RD (2005) Comprehensive algorithm for quantitative real-time polymerase chain reaction. J Comput Biol 12(8):1047-1064
13. Hellemans J, Mortier G, De Paepe A, Speleman F, Vandesompele J (2007) qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data. Genome Biol 8(2):R19
14. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25(4):402-408
15. Huth A, Vennemann B, Fracasso T, Lutz-Bonengel S, Vennemann M (2013) Apparent versus true gene expression changes of three hypoxia-related genes in autopsy derived tissue and the importance of normalisation. Int J Legal Med 127(2):335-344
16. Gibson UE, Heid CA, Williams PM (1996) A novel method for real time quantitative RT-PCR. Genome Res 6(10):995-1001
17. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3 (7):RESEARCH0034
18. Wang Q, Ishikawa T, Michiue T, Zhu BL, Guan DW, Maeda H (2012) Quantitative immunohistochemical analysis of human brain basic fibroblast growth factor, glial fibrillary acidic protein and single-stranded DNA expressions following traumatic brain injury. Forensic Sci Int 221(1-3):142-151
19. Wang Q, Ishikawa T, Michiue T, Zhu BL, Guan DW, Maeda H (2012) Evaluation of human brain damage in fatalities due to extreme environmental temperature by quantification of basic fibroblast growth factor (bFGF), glial fibrillary acidic protein (GFAP), S100beta and single-stranded DNA (ssDNA) immunoreactivities. Forensic Sci Int 219(1-3):259-264
20. Zhao D, Ishikawa T, Quan L, Li DR, Michiue T, Yoshida C, Komatu A, Chen JH, Zhu BL, Maeda H (2008) Tissue-specific differences in mRNA quantification of glucose transporter 1 and vascular endothelial growth factor with special regard to death investigations of fatal injuries. Forensic Sci Int 177(2-3):176-183
21. Miyazato T, Ishikawa T, Michiue T, Maeda H (2012) Molecular pathology of pulmonary surfactants and cytokines in drowning compared with other asphyxiation and fatal hypothermia. Int J Legal Med 126(4):581-587
22. Hayashi T, Ishida Y, Mizunuma S, Kimura A, Kondo T (2009) Differential diagnosis between freshwater drowning and saltwater drowning based on intrapulmonary aquaporin-5 expression. Int J Legal Med 123(1):7-13
23. Chen JH, Michiue T, Ishikawa T, Maeda H (2012) Molecular pathology of natriuretic peptides in the myocardium with special regard to fatal intoxication, hypothermia, and hyperthermia. Int J Legal Med 126(5):747-756
24. Wang Q, Ishikawa T, Michiue T, Zhu BL, Guan DW, Maeda H (2012) Intrapulmonary aquaporin-5 expression as a possible biomarker for discriminating smothering and choking from sudden cardiac death: a pilot study. Forensic Sci Int 220(1-3):154-157
25. Wang Q, Ishikawa T, Michiue T, Zhu BL, Guan DW, Maeda H (2012) Molecular pathology of pulmonary edema after injury in forensic autopsy cases. Int J Legal Med 126(6):875-882
26. Iino M, Nakatome M, Ogura Y, Fujimura H, Kuroki H, Inoue H, Ino Y, Fujii T, Terao T, Matoba R (2003) Real-time PCR quantitation of FE65 a beta-amyloid precursor protein-binding protein after traumatic brain injury in rats. Int J Legal Med 117(3):153-159
27. Ma WX, Yu TS, Fan YY, Zhang ST, Ren P, Wang SB, Zhao R, Pi JB, Guan DW (2011) Time-dependent expression and distribution of monoacylglycerol lipase during the skin-incised wound healing in mice. Int J Legal Med 125(4):549-558
28. Yu TS, Cheng ZH, Li LQ, Zhao R, Fan YY, Du Y, Ma WX, Guan DW (2010) The cannabinoid receptor type 2 is time-dependently expressed during skeletal muscle wound healing in rats. Int J Legal Med 124(5):397-404
29. Kimura A, Ishida Y, Hayashi T, Nosaka M, Kondo T (2011) Estimating time of death based on the biological clock. Int J Legal Med 125(3):385-391
30. Zheng JL, Yu TS, Li XN, Fan YY, Ma WX, Du Y, Zhao R, Guan DW (2012) Cannabinoid receptor type 2 is time-dependently expressed during skin wound healing in mice. Int J Legal Med 126(5):807-814
31. Madea B, Saukko P, Oliva A, Musshoff F (2010) Molecular pathology in forensic medicine - introduction. Forensic Sci Int 203(1-3):3-14
32. Maeda H, Ishikawa T, Michiue T (2011) Forensic biochemistry for functional investigation of death: concept and practical application. Leg Med (Tokyo) 13(2):55-67
33. de Jonge HJ, Fehrmann RS, de Bont ES, Hofstra RM, Gerbens F, Kamps WA, de Vries EG, van der Zee AG, te Meerman GJ, ter Elst A (2007) Evidence based selection of housekeeping genes. PLoS One 2(9):e898
34. Koppelkamm A, Vennemann B, Fracasso T, Lutz-Bonengel S, Schmidt U, Heinrich M (2010) Validation of adequate endogenous reference genes for the normalisation of qPCR gene expression data in human post mortem tissue. Int J Legal Med 124(5):371-380
35. Penna I, Vella S, Gigoni A, Russo C, Cancedda R, Pagano A (2011) Selection of candidate housekeeping genes for normalization in human postmortem brain samples. Int J Mol Sci 12(9):5461-5470
36. Sun JH, Nan LH, Gao CR, Wang YY (2012) Validation of reference genes for estimating wound age in contused rat skeletal muscle by quantitative real-time PCR. Int J Legal Med 126(1):113-120
37. Nielsen S, Smith BL, Christensen EI, Agre P (1993) Distribution of the aquaporin CHIP in secretory and resorptive epithelia and capillary endothelia. Proc Natl Acad Sci U S A 90(15):7275-7279
38. Tait MJ, Saadoun S, Bell BA, Papadopoulos MC (2008) Water movements in the brain: role of aquaporins. Trends Neurosci 31(1):37-43
39. Vajda Z, Promeneur D, Doczi T, Sulyok E, Frokiaer J, Ottersen OP, Nielsen S (2000) Increased aquaporin-4 immunoreactivity in rat brain in response to systemic hyponatremia. Biochem Biophys Res Commun 270(2):495-503
40. Eefsen M, Jelnes P, Schmidt LE, Vainer B, Bisgaard HC, Larsen FS (2010) Brain expression of the water channels aquaporin-1 and -4 in mice with acute liver injury, hyperammonemia and brain edema. Metab Brain Dis 25(3):315-323
41. Yang Y, Estrada EY, Thompson JF, Liu W, Rosenberg GA (2007) Matrix metalloproteinase-mediated disruption of tight junction proteins in cerebral vessels is reversed by synthetic matrix metalloproteinase inhibitor in focal ischemia in rat. J Cereb Blood Flow Metab 27(4):697-709
42. Burek M, Arias-Loza PA, Roewer N, Forster CY (2010) Claudin-5 as a novel estrogen target in vascular endothelium. Arterioscler Thromb Vac Biol 30(2):298-304
43. Zhang H, Adwanikar H, Werb Z, Noble-Haeusslein LJ (2010) Matrix metalloproteinases and neurotrauma: evolving roles in injury and reparative processes. Neuroscientist 16(2):156-170
44. Corry DB, Kiss A, Song LZ, Song L, Xu J, Lee SH, Werb Z, Kheradmand F (2004) Overlapping and independent contributions of MMP2 and MMP9 to lung allergic inflammatory cell egression through decreased CC chemokines. FASEB J 18(9):995-997
45. Nosaka M, Ishida Y, Kimura A, Kuninaka Y, Inui M, Mukaida N, Kondo T (2011) Absence of IFN-gamma accelerates thrombus resolution through enhanced MMP-9 and VEGF expression in mice. J Clin Invest 121(7):2911-2920
46. Reyes R Jr, Wu Y, Lai Q, Mrizek M, Berger J, Jimenez DF, Barone CM, Ding Y (2006) Early inflammatory response in rat brain after peripheral thermal injury. Neurosci Lett 407(1):11-15
47. Koppelkamm A, Vennemann B, Lutz-Bonengel S, Fracasso T, Vennemann M (2011) RNA integrity in post-mortem samples: influencing parameters and implications on RT-qPCR assays. Int J Legal Med 125(4):573-580
48. Zhao D, Zhu BL, Ishikawa T, Quan L, Li DR, Maeda H (2006) Real-time RT-PCR quantitative assays and postmortem degradation profiles of erythropoietin, vascular endothelial growth factor and hypoxia-inducible factor 1 alpha mRNA transcripts in forensic autopsy materials. Leg Med (Tokyo) 8(2):132-136