Molecular Cloning and Characterization of Three Genes Encoding Dihydroflavonol-4-Reductase from Ginkgo biloba in Anthocyanin Biosynthetic Pathway

PLoS ONE

Volumn 8, Issue 8, 2013, Pages

  Hua, Cheng ^a,b,c   Linling, Li ^a,b   Shuiyuan, Cheng ^a,b,c   Fuliang, Cao ^c   Feng, Xu ^a   Honghui, Yuan ^a,b   Conghua, Wu ^a,b  

^a Economic Forest Germplasm Improvement and Comprehensive Utilization of Resources of Hubei Key Lab   (China)

^b HUANGGANG NORMAL UNIVERSITY   (China)

^c NANJING FORESTRY UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ABSCISIC ACID; AMINOLEVULINIC ACID; ANTHOCYANIN; COMPLEMENTARY DNA; DIHYDROFLAVONOL 4 REDUCTASE; DIHYDROFLAVONOL 4 REDUCTASE 1; DIHYDROFLAVONOL 4 REDUCTASE 2; DIHYDROKAEMPHEROL 7 GLUCOSIDE; KAEMPFEROL; LEUCOPELARGONIDIN; OXIDOREDUCTASE; PELARGONIDIN; PHOSPHONIC ACID ESTER; PYCNOGENOL; SALICYLIC ACID; TAXIFOLIN; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CONTROLLED STUDY; DNA SEQUENCE; ENVIRONMENTAL STRESS; GENETIC TRANSCRIPTION; GINKGO BILOBA; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENETIC TREE; PLANT GENE; PLANT LEAF; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING; STAMEN; TISSUE DISTRIBUTION; ULTRAVIOLET B RADIATION;

ALCOHOL OXIDOREDUCTASES; AMINO ACID SEQUENCE; ANTHOCYANINS; BASE SEQUENCE; BIOCATALYSIS; BIOSYNTHETIC PATHWAYS; BLOTTING, WESTERN; CLONING, MOLECULAR; FLAVONOIDS; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, PLANT; GINKGO BILOBA; ISOENZYMES; MOLECULAR SEQUENCE DATA; MULTIGENE FAMILY; PHYLOGENY; PLANT GROWTH REGULATORS; PLANT PROTEINS; QUERCETIN; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY, AMINO ACID; ULTRAVIOLET RAYS;

EID: 84883180182     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0072017     Document Type: Article

References (61)

1. Manach C, Scalbert A, Morand C, Rémésy C, Jiménez L, (2004) Polyphenols: food sources and bioavailability. The American journal of clinical nutrition 79: 727-747.
2. Williams CA, Grayer RJ, (2004) Anthocyanins and other flavonoids. Natural product reports 21: 539-573.
3. Martens S, Knott J, Seitz CA, Janvari L, Yu SN, et al. (2003) Impact of biochemical pre-studies on specific metabolic engineering strategies of flavonoid biosynthesis in plant tissues. Biochemical engineering journal 14: 227-235.
4. Winkel-Shirley B, (2001) It takes a garden. How work on diverse plant species has contributed to an understanding of flavonoid metabolism. Plant physiology 127: 1399-1404.
5. Peer WA, Murphy AS, (2007) Flavonoids and auxin transport: modulators or regulators? Trends in plant science 12: 556-563.
6. Cheng SY, Xu F, Wang Y, (2009) Advances in the study of flavonoids in Ginkgo biloba leaves. J Med Plants Res 3: 1248-1252.
7. Singh K, Kumar S, Yadav SK, Ahuja PS, (2009) Characterization of dihydroflavonol 4-reductase cDNA in tea [Camellia sinensis (L.) O. Kuntze]. Plant Biotechnology Reports 3: 95-101.
8. Cheng H, Li L, Cheng S, Cao F, Wang Y, et al. (2011) Molecular cloning and function assay of a chalcone isomerase gene (GbCHI) from Ginkgo biloba. Plant cell reports 30: 1-14.
9. Winkel-Shirley B, (2002) Biosynthesis of flavonoids and effects of stress. Current Opinion in Plant Biology 5: 218-223.
10. Huang Y, Gou J, Jia Z, Yang L, Sun Y, et al. (2012) Molecular cloning and characterization of two genes encoding dihydroflavonol-4-reductase from Populus trichocarpa. PloS one 7: e30364.
11. Xie DY, Jackson LA, Cooper JD, Ferreira D, Paiva NL, (2004) Molecular and biochemical analysis of two cDNA clones encoding dihydroflavonol-4-reductase from Medicago truncatula. Plant physiology 134: 979-994.
12. Xie D-Y, Sharma SB, Paiva NL, Ferreira D, Dixon RA, (2003) Role of anthocyanidin reductase, encoded by BANYULS in plant flavonoid biosynthesis. Science 299: 396-399.
13. Li H, Qiu J, Chen F, Lv X, Fu C, et al. (2012) Molecular characterization and expression analysis of dihydroflavonol 4-reductase (DFR) gene in Saussurea medusa. Molecular Biology Reports: 1-9.
14. Yoshida K, Iwasaka R, Shimada N, Ayabe S, Aoki T, et al. (2010) Transcriptional control of the dihydroflavonol 4-reductase multigene family in Lotus japonicus. Journal of plant research 123: 801-805.
15. Peters DJ, Constabel CP, (2002) Molecular analysis of herbivore-induced condensed tannin synthesis: cloning and expression of dihydroflavonol reductase from trembling aspen (Populus tremuloides). The Plant Journal 32: 701-712.
16. Smith J, Luo Y, (2004) Studies on molecular mechanisms of Ginkgo biloba extract. Applied microbiology and biotechnology 64: 465-472.
17. van Beek TA, (2002) Chemical analysis of Ginkgo biloba leaves and extracts. Journal of Chromatography A 967: 21-55.
18. Stafford HA, Lester HH, (1985) Flavanol biosynthesis: the conversion of (+)-dihydromyricetin to its flavan-3, 4-diol (leucodelphinidin) and to (+)-gallocatechin by reductases extracted from tissue cultures of Ginkgo biloba and Pseudotsuga menziesii. Plant physiology 78: 791-794.
19. Xu F, Cheng H, Cai R, Li LL, Chang J, et al. (2008) Molecular cloning and function analysis of an anthocyanidin synthase gene from Ginkgo biloba, and its expression in abiotic stress responses. Molecules and Cells 26: 536-547.
20. Murray JR, Hackett WP, (1991) Dihydroflavonol reductase activity in relation to differential anthocyanin accumulation in juvenile and mature phase Hedera helix L. Plant Physiology. 97: 343.
21. Pang Y, Peel GJ, Wright E, Wang Z, Dixon RA, (2007) Early steps in proanthocyanidin biosynthesis in the model legume Medicago truncatula. Plant physiology 145: 601-615.
22. Tamura K, Dudley J, Nei M, Kumar S, (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24: 1596-1599.
23. Xu F, Li L, Zhang W, Cheng H, Sun N, et al. (2012) Isolation, characterization, and function analysis of a flavonol synthase gene from Ginkgo biloba. Molecular Biology Reports 39: 2285-2296.
24. Cheng S, Wang Y, Li J, Fei Y, Zhu G, (2004) Study on the relationship between the endogenous hormones and flavonoids in Ginkgo biloba Leaf. Scientia Silvae Sinicae 40: 45-49.
25. Wang LJ, Jiang WB, Huang BJ, (2004) Promotion of 5-aminolevulinic acid on photosynthesis of melon (Cucumis melo) seedlings under low light and chilling stress conditions. Physiologia plantarum 121: 258-264.
26. Xu F, Cheng H, Cai R, Li LL, Chang J, et al. (2008) Molecular cloning and function analysis of an anthocyanidin synthase gene from Ginkgo biloba, and its expression in abiotic stress responses. Molecules and Cells 26: 536-547.
27. Xu F, Cai R, Cheng S, Du H, Wang Y, (2008) Molecular cloning, characterization and expression of phenylalanine ammonia-lyase gene from Ginkgo biloba. African Journal of Biotechnology 7: 721-729.
28. Cheng S, Wang Y, Liu W, Du H, Chen K, (2005) Effects of plant growth regulators on phenylalanine ammonia-lyase (PAL) activities in leaves of Ginkgo biloba in vitro. Journal of Plant Resources and Environment 14: 20-22.
29. Cheng S, Wang Y, Fei Y, Zhu G, (2004) Studies on the effects of different treatments on flavonoids contents in Ginkgo biloba leaves and their regulating mechanism. Journal of Fruit Science 21: 116-119.
30. Yan W, Shuiyuan C, (2002) Studies on the effects of regulating measures on the flavonoids contents in Ginkgo biloba leaves. Hubei Agricultural Sciences 25: 103-105.
31. Hayat S, Ali B, Ahmad A (2007) Salicylic acid: biosynthesis, metabolism and physiological role in plants. 1-14 p.
32. Meyer P, Heidmann I, Forkmann G, Saedler H, (1987) A new petunia flower colour generated by transformation of a mutant with a maize gene. Nature 330: 677-678.
33. Johnson ET, Yi H, Shin B, Oh BJ, Cheong H, et al. (1999) Cymbidium hybrida dihydroflavonol 4-reductase does not efficiently reduce dihydrokaempferol to produce orange pelargonidin-type anthocyanins. The Plant Journal 19: 81-85.
34. Martens S, Teeri T, Forkmann G, (2002) Heterologous expression of dihydroflavonol 4-reductases from various plants. FEBS letters 531: 453-458.
35. Johnson ET, Ryu S, Yi H, Shin B, Cheong H, et al. (2001) Alteration of a single amino acid changes the substrate specificity of dihydroflavonol 4-reductase. The Plant Journal 25: 325-333.
36. Shimada N, Sasaki R, Sato S, Kaneko T, Tabata S, et al. (2005) A comprehensive analysis of six dihydroflavonol 4-reductases encoded by a gene cluster of the Lotus japonicus genome. Journal of experimental botany 56: 2573-2585.
37. Liu J, Feng Q, Zhang J, (2006) Dihydroflavonol 4-reductase gene (DFR) and flower color modulation. Plant Physiology Communications 41: 715-719.
38. Liew CF, Loh CS, Goh CJ, Lim SH, (1998) The isolation, molecular characterization and expression of dihydroflavonol 4-reductase cDNA in the orchid (Bromheadia finlaysoniana). Plant Science 135: 161-169.
39. Ostergaard L, Lauvergeat V, Naested H, Mattsson O, Mundy J, (2001) Two differentially regulated Arabidopsis genes define a new branch of the DFR superfamily. Plant Science 160: 463-472.
40. Liu G, Lei X, Zhu Q, Zhong G, Guo T, et al. (2009) Cloning and sequence analysis of a DFR gene from Fagopyrum dibotrys(D.Don) Hara. Scientia Agricultura Sinica 42: 55-63.
41. Huits HSM, Gerats AGM, Kreike MM, Mol JNM, Koes RE, (1994) Genetic control of dihydroflavonol 4-reductase gene expression in Petunia hybrida. The Plant Journal 6: 295-310.
42. Nakatsuka A, Izumi Y, Yamagishi M, (2003) Spatial and temporal expression of chalcone synthase and dihydroflavonol 4-reductase genes in the Asiatic hybrid lily. Plant Science 165: 759-767.
43. Tang LK, Chu H, Yip WK, Yeung EC, Lo C, (2009) An anther-specific dihydroflavonol 4-reductase-like gene (DRL1) is essential for male fertility in Arabidopsis. New Phytologist 181: 576-587.
44. Gaffney T, Friedrich L, Vernooij B, Negrotto D, Nye G, et al. (1993) Requirement of salicylic acid for the induction of systemic acquired resistance. Science 261: 754-756.
45. Fofana B, McNally DJ, Labbé C, Boulanger R, Benhamou N, et al. (2002) Milsana-induced resistance in powdery mildew-infected cucumber plants correlates with the induction of chalcone synthase and chalcone isomerase. Physiological and molecular plant pathology 61: 121-132.
46. Meer I, Stuitje A, Mol J, Verma D (1993) Regulation of general phenylpropanoid and flavonoid gene expression. 125-155 p.
47. Skriver K, Mundy J, (1990) Gene expression in response to abscisic acid and osmotic stress. The Plant Cell 2: 503.
48. Wei Y-Z, Hu F-C, Hu G-B, Li X-J, Huang X-M, et al. (2011) Differential expression of anthocyanin biosynthetic genes in relation to anthocyanin accumulation in the pericarp of Litchi Chinensis Sonn. PloS one 6: e19455.
49. El-Kereamy A, Chervin C, Roustan JP, Cheynier V, Souquet JM, et al. (2003) Exogenous ethylene stimulates the long-term expression of genes related to anthocyanin biosynthesis in grape berries. Physiologia plantarum 119: 175-182.
50. Hotta Y, Tanaka T, Takaoka H, Takeuchi Y, Konnai M, (1997) Promotive effects of 5-aminolevulinic acid on the yield of several crops. Plant Growth Regulation 22: 109-114.
51. Von Wettstein D, Gough S, Kannangara CG, (1995) Chlorophyll biosynthesis. The Plant Cell 7: 1039.
52. Nishihara E, Kondo K, Parvez MM, Takahashi K, Watanabe K, et al. (2003) Role of 5-aminolevulinic acid (ALA) on active oxygen-scavenging system in NaCl-treated spinach (Spinacia oleracea). Journal of plant physiology 160: 1085-1091.
53. Cheng S, Wang Y, Li J, Tao Z, (2001) Study on the relationship between the flavonoids and pigments in Ginkgo biloba leaf. Scientia Silvae Sinicae 37: 31-34.
54. Awad MA, Wagenmakers PS, de Jager A, (2001) Effects of light on flavonoid and chlorogenic acid levels in the skin of [] Jonagold'apples. Scientia Horticulturae 88: 289-298.
55. Feng X (2008) Cloning and expression of GbPAL and GbANS genes and effect of ALA on the content of flavonoids in Ginkgo biloba. Taian Shandong Province: Shandong Agricultural University.
56. Jaakola L, Hohtola A, (2010) Effect of latitude on flavonoid biosynthesis in plants. Plant, Cell & Environment 33: 1239-1247.
57. Cancan Z (2010) Effects of environmental factors on the growth and principal medicinal compositions of Ginkgo biloba seedlings. Nanjing Jiangsu province: Nanjing Forestry University.
58. Guo J, Wang M-H, (2010) Ultraviolet A-specific induction of anthocyanin biosynthesis and PAL expression in tomato (Solanum lycopersicum L.). Plant Growth Regulation 62: 1-8.
59. Bashandy T, Taconnat L, Renou JP, Meyer Y, Reichheld JP, (2009) Accumulation of flavonoids in an ntra ntrb mutant leads to tolerance to UV-C. Molecular Plant 2: 249-258.
60. Wang Q, Li X, Wang L, Sun F, Shen X, (2008) Dynamic changes of anthocyanin and the relevant biosynthesis enzymes in Padus virginiana 'Schubert' leaves. Scientia Silvae Sinicae 44: 45-49.
61. Kim S, Yoo KS, Pike LM, (2005) Development of a PCR-based marker utilizing a deletion mutation in the dihydroflavonol 4-reductase (DFR) gene responsible for the lack of anthocyanin production in yellow onions (Allium cepa). Theoretical and Applied Genetics 110: 588-595.