Alteration of a single amino acid changes the substrate specificity of dihydroflavonol 4-reductase

Plant Journal

Volumn 25, Issue 3, 2001, Pages 325-333

  Johnson, Eric T ^a   Ryu, Sunhyo ^b   Yi, Hankuil ^a   Shin, Byongchul ^a   Cheong, Hyeonsook ^b   Choi, Giltsu ^a  

^a Kumho Life and Environmental Science Laboratory   (South Korea)

^b Chosun University   (South Korea)

Author keywords

Anthocyanin; Dihydroflavonol 4 reductase; Pelargonidin; Site directed mutagenesis; Substrate specificity

Indexed keywords

AMINO ACID SUBSTITUTION; ANTHOCYANIN; CHIMERISM; DIHYDROFLAVONOL 4 REDUCTASE; DIHYDROKAEMPFEROL; ENZYME SPECIFICITY; FLOWER COLOR; GERBERA; PETUNIA; REDUCTION;

ALCOHOL OXIDOREDUCTASES; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANGIOSPERMS; BASE SEQUENCE; DNA PRIMERS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

GERBERA; PETUNIA;

EID: 0035115023     PISSN: 09607412     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-313X.2001.00962.x     Document Type: Article

References (39)

1. Expansion of the mammalian 3 beta-hydroxysteroid dehydrogenase/plant dihydroflavonol reductase superfamily to include a bacterial cholesterol dehydrogenase, a bacterial UDP-galactose-4-epimerase, and open reading frames in vaccinia virus and fish lymphocystis disease virus
2. Flavonoid synthesis in Petunia hybrida: Partial characterization of dihydroflavonol-4-reductase genes
3. The refined crystal structure of Drosophila lebanonensis alcohol dehydrogenase at 1.9 A resolution
4. Structure of 3 alpha-hydroxysteroid/dihydrodiol dehydrogenase complexed with NADP+
5. Isolation and characterization of a flavonoid 3′-hydroxylase cDNA clone corresponding to the Ht1 locus of Petunia hybrida
6. Interactions among enzymes of the Arabidopsis flavonoids biosynthetic pathway
7. JPred: A consensus secondary structure prediction server
8. Flavonoids and isoflavonoids - A gold mine for metabolic engineering
9. The fascinating complexities of steroid-binding enzymes
10. Transgene inactivation in Petunia hybrida is influenced by the properties of the foreign gene
11. Structure of chalcone synthase and the molecular basis of plant polyketide biosynthesis
12. Distinct substrate specificity of dihydroflavonol 4-reductase from flowers of Petunia hybrida
13. Genetic control of the conversion of dihydroflavonols into flavonols and anthocyanins in flowers of Petunia hybrida
14. A novel aromatic alcohol dehydrogenase in higher plants: Molecular cloning and expression
15. Cloning of cDNA coding for dihydroflavonol-4-reductase (DFR) and characterization of dfr expression in the corollas of Gerbera hybrida var. Regina (Compositae)
16. Enzymatic reduction of (+) -dihydroflavonols to flavan-3,4-cis-diols with flower extracts from Matthiola incana and its role in anthocyanin biosynthesis
17. Cloning and expression of cytochrome P450 genes controlling flower colour
18. Genetics and biochemistry of anthocyanin biosynthesis
19. Blue roses - A pigment of our imagination?
20. Genetic control of dihydroflavonol 4-reductase gene expression in Petunia hybrida
21. Crystal structure of cis-biphenyl-2,3-dihydrodiol-2,3-dehydrogenase from a PCB degrader at 2.0 A resolution
22. Cymbidium hybrida dihydroflavonol 4-reductase does not efficiently reduce dihydrokaempferol to produce orange pelargonidin-type anthocyanins
23. Short-chain dehydrogenases/reductases (SDR)
24. Cinnamoyl CoA reductase, the first committed enzyme of the lignin branch biosynthetic pathway: Cloning, expression and phylogenetic relationships
25. A new petunia flower colour generated by transformation of a mutant with a maize gene
26. Novel coloured flowers
27. How genes paint flowers and seeds
28. Structure and function of 3 alpha-hydroxysteroid dehydrogenase
29. Flavonoids biosynthesis: New functions for an old pathway
30. Analysis of Arabidopsis mutants deficient in flavonoid biosynthesis
31. Enzymatic conversion of dihydroflavonols to flavan-3,4-diols using flower extracts of Dianthus caryophyllus L. (carnation)
32. Crystal structures of the binary and ternary complexes of 7 alpha-hydroxysteroid dehydrogenase from Escherichia coli
33. Molecular cloning and characterization of Rosa hybrida dihydroflavonol 4-reductase gene
34. Metabolic engineering to modify flower color
35. Crystal structures of the oxidized and reduced forms of UDP-galactose 4-epimerase isolated from Escherichia coli
36. High-resolution X-ray structure of UDP-galactose 4-epimerase complexed with UDP-phenol
37. Structural analysis of UDP-sugar binding to UDP-Galactose 4-epimerase from Escherichia coli
38. Clustal W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-speific gap penalties and weight matrix choice
39. Macromolecular organization of the primary and secondary pathways of aromatic amino acid biosynthesis